Antonie van Leeuwenhoek

, Volume 107, Issue 5, pp 1331–1336 | Cite as

Sphingobacterium yamdrokense sp. nov., isolated from Lake Yamdrok

  • Na Xiao
  • Yongqin Liu
  • Zhengquan Gu
  • Xiaobo Liu
  • Nianzhi Jiao
  • Hongcan Liu
  • Yuguang Zhou
  • Liang Shen
Original Paper

Abstract

A strictly aerobic and facultatively psychrophilic bacterium, strain 3-0-1T, was isolated from Lake Yamdrok on the Tibetan Plateau, China. Cells were Gram-negative and short-rod-shaped. Strain 3-0-1T formed circular, opaque, yellow colonies, and grew at 0–5 % (w/v) NaCl, pH 5.0–11.0 and 5–35 °C. Phylogenetic analysis based on 16S rRNA gene sequences of various species with validly published names showed that strain 3-0-1T belongs to the genus Sphingobacterium, family Sphingobacteriaceae, sharing the highest similarity with Sphingobacterim nematocida M-SX103T (96.62 %) and less than 95 % similarity with all the other species in the genus Sphingobactrium. The predominant isoprenoid quinone of strain 3-0-1T was identified as menaquinone 7 (MK-7), the major polar lipid was identified as phosphatidylethanolamine and the DNA G+C content was determined to be 42.5 mol%. The predominant cellular fatty acids were identified as summed feature 3 (C16:1ω7c and/or C16:1ω6c) (>30 %), iso-C15:0 (>20 %) and iso-C17:0 3-OH (>10 %). Based on the variability of phylogenetic and phenotypic characteristics, strain 3-0-1T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium yamdrokense sp. nov. is proposed. The type strain is 3-0-1T (=JCM 19397T = CGMCC 1.12560T).

Keywords

Sphingobacterium yamdrokense sp. nov. Polyphasic taxomony High altitude lake 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (41425004, 41171050, 41201059) and the National Basic Research Program of China (2012CB956100).

Supplementary material

10482_2015_428_MOESM1_ESM.pptx (237 kb)
Supplementary material 1 (PPTX 236 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Na Xiao
    • 1
    • 2
  • Yongqin Liu
    • 2
    • 3
  • Zhengquan Gu
    • 1
    • 2
  • Xiaobo Liu
    • 1
    • 2
  • Nianzhi Jiao
    • 4
  • Hongcan Liu
    • 5
  • Yuguang Zhou
    • 5
  • Liang Shen
    • 1
    • 2
  1. 1.Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.CAS Center for Excellence in Tibetan Plateau Earth SciencesBeijingChina
  4. 4.State Key Laboratory of Marine Environmental ScienceXiamen UniversityXiamenPeople’s Republic of China
  5. 5.Institute of Microbiology, China General Microbiological Culture Collection CenterChinese Academy of SciencesBeijingPeople’s Republic of China

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