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Antonie van Leeuwenhoek

, Volume 107, Issue 5, pp 1271–1280 | Cite as

Rhodococcus agglutinans sp. nov., an actinobacterium isolated from a soil sample

  • Qian-Qian Guo
  • Hong Ming
  • Xiao-Ling Meng
  • Yan-Yan Duan
  • Rui GaoEmail author
  • Jian-Xin Zhang
  • Jian-Rong Huang
  • Wen-Jun Li
  • Guo-Xing NieEmail author
Original Paper

Abstract

A Gram-positive, aerobic, non-motile and non-spore forming strain, designated CFH S0262T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. A polyphasic approach was used to study the taxonomic position of this new isolate. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain CFH S0262T belongs to the genus Rhodococcus and clustered with Rhodococcus soli DSD51WT, Rhodococcus hoagii NBRC 103062T, Rhodococcus defluvii CallT and Rhodococcus kunmingensis YIM 45607T (98.7, 98.5, 97.9 and 97.6 % similarities, respectively). Strain CFH S0262T could grow in the presence of NaCl (0–4 %, optimum 0–3 %), at pH 6.0–8.0 (optimum, pH 7.0) and at 10–40 °C (optimum, 28 °C). The predominant menaquinones of strain CFH S0262T were identified as MK-8 (H2) and MK-8 (H4). The major fatty acids (≥10 %) were found to be C16:0 and C18:1ω9c. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, a glycolipid and two unidentified phospholipids. The DNA G+C content was determined to be 71.4 mol%. Based on a comparative analysis of phenotypic and genotypic characteristics, in combination with low values of DNA–DNA hybridization between strain CFH S0262T and its closest neighbours, it is proposed that strain CFH S0262T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus agglutinans sp. nov., is proposed, with the type strain CFH S0262T (=CCTCC AB2014297T=KCTC 39118T).

Keywords

Rhodococcus agglutinans sp. nov. Rhodococcus 16S rRNA gene Polyphasic taxonomy Halong Bay 

Notes

Acknowledgments

The authors are grateful to Prof. Hans-Peter Klenk (DSMZ, Germany) and Dr. Tomohiko Tamura (NBRC, Japan) for their kind providing the reference type strains and Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help with the Latin etymology for the new species. This research was supported by Natural Science Foundation of China (No. 31372545), Project of China tobacco Yunnan industrial Co. Ltd. (No. 2014YL01), Program for Innovative Research Team (in Science and Technology) in University of Henan Province (14IRTSTHN013), Plan for Scientific Innovation Talent of Henan Province (154100510010), Scientific Research Fund of Xinxiang Medical University (2013QN126), and Research Project of Education Department of Henan Province of China (2011A180025).

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Supplementary material

10482_2015_421_MOESM1_ESM.doc (3 mb)
Supplementary material 1 (DOC 3116 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Qian-Qian Guo
    • 1
  • Hong Ming
    • 2
  • Xiao-Ling Meng
    • 1
  • Yan-Yan Duan
    • 1
  • Rui Gao
    • 3
    Email author
  • Jian-Xin Zhang
    • 1
  • Jian-Rong Huang
    • 1
  • Wen-Jun Li
    • 4
  • Guo-Xing Nie
    • 1
    Email author
  1. 1.College of FisheriesHenan Normal UniversityXinxiangPeople’s Republic of China
  2. 2.College of Life Sciences and TechnologyXinxiang Medical UniversityXinxiangPeople’s Republic of China
  3. 3.China Tobacco Yunnan Industrial Co., LtdKunmingPeople’s Republic of China
  4. 4.State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and EvolutionSun Yat-Sen UniversityGuangzhouPeople’s Republic of China

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