Antonie van Leeuwenhoek

, Volume 107, Issue 5, pp 1189–1196 | Cite as

Tamlana nanhaiensis sp. nov., isolated from surface seawater collected from the South China Sea

  • Xiupian Liu
  • Qiliang Lai
  • Yaping Du
  • Guangyu Li
  • Fengqin Sun
  • Zongze Shao
Original Paper
  • 162 Downloads

Abstract

A polyphasic taxonomic study was performed on a strain, designated FHC16T, which was isolated from surface seawater collected from the South China Sea. Cells of strain FHC16T are Gram stain-negative, oxidase- and catalase-positive and non-motile rods. Growth was observed at 15–37 °C (optimum, 25–30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 0–5  % (w/v) NaCl (optimum, 3 %). 16S rRNA gene sequence analysis showed that strain FHC16T is most closely related to Tamlana sedimentorum JCM 19808T (98.2 % sequence similarity). The ANI value between strain FHC16T and T. sedimentorum JCM 19808T was found to be 81.82–81.81 %. The DNA–DNA hybridization estimated value between strain FHC16T and T. sedimentorum JCM 19808T was determined to be 25.8 ± 2.41 %. The principal fatty acids (>5 % of the total) were found to be iso-C15:0, iso G-C15:1, iso-C17:0 3-OH, iso-C15:0 3-OH and summed feature 3 (comprising C16:1ω7c/C16:1ω6c). The strain was found to have MK-6 as the major respiratory menaquinone, which is consistent with the other three recognized Tamlana species, T. sedimentorum, Tamlana crocina and Tamlana agarivorans. The polar lipids were found to comprise phosphatidylethanolamine, one unidentified aminophospholipid, two unidentified aminolipids and seven unidentified lipids. The G+C content of the chromosomal DNA was determined to be 34.2 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain FHC16T is considered to represent a novel species of the genus Tamlana, for which the name Tamlana nanhaiensis sp. nov. is proposed. The type strain is FHC16T (\( = \)LMG 27420T = CGMCC 1.12469T = MCCC 1A06648T).

Keywords

Tamlana nanhaiensis sp. nov. Surface seawater sample Taxonomy The South China Sea 

Abbreviation

MCCC

Marine Culture Collection of China

Notes

Acknowledgments

This work was financially supported by the fund of National Infrastructure of Microbial Resources (No. NIMR2014-9).

Supplementary material

10482_2015_410_MOESM1_ESM.docx (523 kb)
Supplementary material 1 (DOCX 524 kb)
10482_2015_410_MOESM2_ESM.docx (24 kb)
Supplementary material 2 (DOCX 25 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Xiupian Liu
    • 1
  • Qiliang Lai
    • 1
  • Yaping Du
    • 1
  • Guangyu Li
    • 1
  • Fengqin Sun
    • 1
  • Zongze Shao
    • 1
  1. 1.State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOAKey Laboratory of Marine Genetic Resources of Fujian ProvinceXiamenChina

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