Kushneria pakistanensis sp. nov., a novel moderately halophilic bacterium isolated from rhizosphere of a plant (Saccharum spontaneum) growing in salt mines of the Karak area in Pakistan
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The taxonomic position of a Gram-stain negative, moderately halophilic bacterium, designated NCCP-934T, was investigated using polyphasic taxonomic approach. The strain NCCP-934T was isolated from rhizosphere of a plant (Saccharum spontaneum, family Poaceae) growing in salt mines area in the Karak district of Khyber Pakhtunkhwa Province, Pakistan. Cells of strain NCCP-934T are rod shaped and motile. The bacterium is strictly aerobic, can grow at a temperature range of 10–40 °C (optimum at 30–33 °C) and in a pH range of 6.0–10.5 (optimum pH 7.0–9.0). The strain can tolerate 1–30 % (w/v) NaCl (optimal growth occurs in the presence of approximately 3–9 % NaCl). The phylogenetic analysis based on the 16S rRNA gene sequence, showed that strain NCCP-934T belongs to the genus Kushneria with the highest sequence similarity to K. marisflavi SW32T (98.9 %), K. indalinina CG2.1T (98.7 %), K. avicenniae MW2aT (98.4 %) and less than 97 % similarity with other related species (94.7 % with the type species of the genus, K. aurantia A10T). DNA–DNA relatedness between strain NCCP-934T and the type strains of the closely related species was lower than 18 %. The chemotaxonomic data (major respiratory quinone, Q9; predominant fatty acids, C18:1 ω7c and C16:0 followed by C12:0 3-OH and Summed features 3 (C16:1 ω7c/iso-C15:0 2-OH); major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanol, phosphatidylserine, phosphatidylinositol and three polar lipid of unknown structure) supported the affiliation of strain NCCP-934T within the genus Kushneria. The DNA G+C content of strain NCCP-934T was 59.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain NCCP-934T can be distinguished from the closely related taxa and thus represents a novel species in the genus Kushneria, for which the name Kushneria pakistanensis sp. nov. is proposed, with the type strain NCCP-934T (=LMG 28525T = KCTC 42082T = JCM 18802T).
KeywordsHalophilic bacterium Karak salt mines Kushneria pakistanensis
This work was supported partly by financial assistance from PSDP funded Project Research for Agricultural Development Project (RADP) under a sub-project (Grant No. CS-55/RADP/PARC) entitled “Establishment of Microbial Bio-Resource Laboratories: National Culture Collection of Pakistan (NCCP)” from Pakistan Agricultural Research Council (PARC), Islamabad, Pakistan and partially from Japan Society for Promotion of Science (JSPS) under fellowship program to I.A.
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