Antonie van Leeuwenhoek

, Volume 107, Issue 4, pp 991–1000 | Cite as

Kushneria pakistanensis sp. nov., a novel moderately halophilic bacterium isolated from rhizosphere of a plant (Saccharum spontaneum) growing in salt mines of the Karak area in Pakistan

  • Asia Bangash
  • Iftikhar Ahmed
  • Saira Abbas
  • Takuji Kudo
  • Armghan Shahzad
  • Toru Fujiwara
  • Moriya Ohkuma
Original Paper

Abstract

The taxonomic position of a Gram-stain negative, moderately halophilic bacterium, designated NCCP-934T, was investigated using polyphasic taxonomic approach. The strain NCCP-934T was isolated from rhizosphere of a plant (Saccharum spontaneum, family Poaceae) growing in salt mines area in the Karak district of Khyber Pakhtunkhwa Province, Pakistan. Cells of strain NCCP-934T are rod shaped and motile. The bacterium is strictly aerobic, can grow at a temperature range of 10–40 °C (optimum at 30–33 °C) and in a pH range of 6.0–10.5 (optimum pH 7.0–9.0). The strain can tolerate 1–30 % (w/v) NaCl (optimal growth occurs in the presence of approximately 3–9 % NaCl). The phylogenetic analysis based on the 16S rRNA gene sequence, showed that strain NCCP-934T belongs to the genus Kushneria with the highest sequence similarity to K. marisflavi SW32T (98.9 %), K. indalinina CG2.1T (98.7 %), K. avicenniae MW2aT (98.4 %) and less than 97 % similarity with other related species (94.7 % with the type species of the genus, K. aurantia A10T). DNA–DNA relatedness between strain NCCP-934T and the type strains of the closely related species was lower than 18 %. The chemotaxonomic data (major respiratory quinone, Q9; predominant fatty acids, C18:1 ω7c and C16:0 followed by C12:0 3-OH and Summed features 3 (C16:1 ω7c/iso-C15:0 2-OH); major polar lipids, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanol, phosphatidylserine, phosphatidylinositol and three polar lipid of unknown structure) supported the affiliation of strain NCCP-934T within the genus Kushneria. The DNA G+C content of strain NCCP-934T was 59.2 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain NCCP-934T can be distinguished from the closely related taxa and thus represents a novel species in the genus Kushneria, for which the name Kushneria pakistanensis sp. nov. is proposed, with the type strain NCCP-934T (=LMG 28525T = KCTC 42082T = JCM 18802T).

Keywords

Halophilic bacterium Karak salt mines Kushneria pakistanensis 

Notes

Acknowledgments

This work was supported partly by financial assistance from PSDP funded Project Research for Agricultural Development Project (RADP) under a sub-project (Grant No. CS-55/RADP/PARC) entitled “Establishment of Microbial Bio-Resource Laboratories: National Culture Collection of Pakistan (NCCP)” from Pakistan Agricultural Research Council (PARC), Islamabad, Pakistan and partially from Japan Society for Promotion of Science (JSPS) under fellowship program to I.A.

Supplementary material

10482_2015_391_MOESM1_ESM.docx (202 kb)
Supplementary material 1 (DOCX 201 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Asia Bangash
    • 1
    • 2
  • Iftikhar Ahmed
    • 1
    • 3
    • 4
  • Saira Abbas
    • 1
    • 3
    • 4
  • Takuji Kudo
    • 3
  • Armghan Shahzad
    • 1
  • Toru Fujiwara
    • 4
  • Moriya Ohkuma
    • 3
  1. 1.National Culture Collection of Pakistan, National Institute for Genomics and Advanced Biotechnology (NIGAB)National Agricultural Research Centre (NARC)IslamabadPakistan
  2. 2.Department of BiochemistryQuaid-e-Azam UniversityIslamabadPakistan
  3. 3.Japan Collection of Microorganisms, RIKEN BioResource CenterTsukubaJapan
  4. 4.Laboratory of Plant Nutrition and Fertilizers, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan

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