Antonie van Leeuwenhoek

, Volume 107, Issue 4, pp 971–977 | Cite as

Geosporobacter ferrireducens sp. nov., an anaerobic iron-reducing bacterium isolated from an oil-contaminated site

  • Heeji Hong
  • So-Jeong Kim
  • Ui-Gi Min
  • Yong-Jae Lee
  • Song-Gun Kim
  • Man-Young Jung
  • Yong-Seok Seo
  • Sung-Keun Rhee
Original Paper

Abstract

In this study, an alkaliphilic and heterotrophic iron-reducing bacterial strain, IRF9T, was isolated from an oil-contaminated soil in the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IRF9T belongs to the genus Geosporobacter in the family Clostridiaceae and is most closely related to Geosporobacter subterraneus VNs68T (96.9 % sequence similarity). Cells of strain IRF9T were observed to be straight or curved rod-shaped, motile and Gram-negative. Optimal growth of strain IRF9T was observed at pH 9.0–9.5 and 40 °C. The strain was found to grow within pH and temperature ranges of 6.5–10.0 and 25–45 °C, respectively. NaCl was not required for growth. Fe(III), but not sulfate, thiosulfate or elemental sulfur can be used by strain IRF9T as an electron acceptor. A limited number of carbohydrates and amino acids, including d-glucose, d-fructose, d-mannitol, d-ribose and l-arginine, support growth of strain IRF9T. The main fatty acids (>10 %) of strain IRF9T were identified as C14:0 (18.4 %), C16:1 cis9 (13.6 %), C16:0 (12.4 %) and C16:0 dimethyl acetal (17.7 %). Major respiratory quinone was identified as menaquinone MK-5 (V-H2). The main polar lipids were found to be phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content of strain IRF9T was determined to be 37.2 mol%, which is lower than that of G. subterraneus VNs68T (42.2 mol%). Based on phenotypic, chemotaxonomic, and phylogenetic studies, we conclude that strain IRF9T (=JCM 19987T = KCTC 15395T) represents a new species of the genus Geosporobacter, for which we propose the name Geosporobacter ferrireducens sp. nov.

Keywords

Iron-reducing bacterium Oil-contaminated soil Geosporobacter ferrireducens 

Notes

Acknowledgments

This work was supported by Mid-career Researcher Program (NRF-2013R1A2A2A05006754) through a National Research Foundation (NRF) grant, funded by the Ministry of Education, Science, and Technology (MEST), the Energy Efficiency & Resources Core Technology Program (20132020000170) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted from the Ministry of Trade, Industry & Energy, and a Grant (NIBR No. 2012-02-057) from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea.

Supplementary material

10482_2015_389_MOESM1_ESM.pdf (112 kb)
Fig. S1. Two-dimensional TLC showing the polar lipid profiles of strain IRF9T and G. subterraneus JCM 14037T stained with molybdatophosphoric acid (Merck). Solvents used were as follows: first direction, chloroform/methanol/water (65:25:4 by vol.); second direction, chloroform/methanol/acetic acid/water (80:12:15:4 by vol.). (a), strain IRF9T; (b), G. subterraneus JCM 14037T. PE, phosphatidylethanolamine; DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; AL, unidentified aminolipid; PL, unidentified phospholipid; L, unidentified polar lipid; L1-2, unidentified polar lipids.Supplementary material 1 (PDF 112 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Heeji Hong
    • 1
  • So-Jeong Kim
    • 1
  • Ui-Gi Min
    • 1
  • Yong-Jae Lee
    • 2
  • Song-Gun Kim
    • 2
    • 3
  • Man-Young Jung
    • 1
  • Yong-Seok Seo
    • 4
  • Sung-Keun Rhee
    • 1
  1. 1.Department of MicrobiologyChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.Microbial Resources Center/KCTCKorea Research Institute of Bioscience and BiotechnologyTaejonRepublic of Korea
  3. 3.University of Science and TechnologyTaejonRepublic of Korea
  4. 4.Department of Earth and Environmental SciencesChungbuk National UniversityCheongjuRepublic of Korea

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