Pedobacter lotistagni sp. nov. isolated from lotus pond water
- 168 Downloads
A light-yellow coloured, Gram-stain negative, rod-shaped, aerobic, non-motile bacterium, designated THG-DN6.8T, was isolated from a lotus pond near Donghaksa temple in Daejeon, South Korea. The strain was found to grow well on nutrient agar, optimally at pH 6.0–7.5, at temperature 25–28 °C and in the presence of 0.5 % NaCl. Based on 16S rRNA gene sequence analysis, strain THG-DN6.8T was found to share the highest sequence similarity with Pedobacter koreensis KCTC 12536T, followed by Pedobacter glacialis CCTCC AB 2012941T, Pedobacter kyungheensis KACC 16221T, Pedobacter caeni LMG 22862T, Pedobacter insulae KCTC 12820T and Pedobacter boryungensis KCTC 23344 T. The DNA G+C content was determined to be 36.8 mol%. In DNA–DNA hybridization tests, the DNA relatedness between strain THG-DN6.8T and its closest phylogenetic neighbour P. koreensis was found to be below 10 %. The predominant isoprenoid quinone was identified as menaquinone MK-7 and the major polar lipid as phosphatidylethanolamine. The major fatty acids of strain THG-DN6.8T were identified as iso-C15:0, C16:0, C18:0 and C16:1ω6c and/or C16:1ω7c (summed feature 3). On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain THG-DN6.8T is considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter lotistagni sp. nov. is proposed. The type strain is THG-DN6.8T (= KCTC 42229T = JCM 30354T).
KeywordsPedobacter lotistagni Gram-staining-negative Menaquinone MK-7 16S rRNA
This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).
- Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high–performance liquid chromatography. Int J Syst Bacteriol 39:159–167Google Scholar
- Moore DD, Dowhan D (1995) Preparation and Analysis of DNA. In: Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current Protocols in Molecular Biology, Wiley, New York, pp 2–11Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, NewarkGoogle Scholar
- Skerman VBD (1967) A guide to the identification of the genera of bacteria, 2nd edn. Williams and Wilkins, BaltimoreGoogle Scholar
- Steyn PL, Segers P, Vancanneyt M, Sandra P, Kersters K, Joubert JJ (1998) Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48:165–177CrossRefPubMedGoogle Scholar
- Zhou Z, Jiang F, Wang S, Peng F, Dai J, Li W, Fang C (2012) Pedobacter arcticus sp. nov., a facultative psychrophile isolated from Arctic soil, and emended descriptions of the genus Pedobacter, Pedobacter heparinus, Pedobacter daechungensis, Pedobacter terricola, Pedobacter glucosidilyticus and Pedobacter lentus. Int J Syst Evol Microbiol 62:1963–1969CrossRefPubMedGoogle Scholar