Antonie van Leeuwenhoek

, Volume 107, Issue 3, pp 723–729 | Cite as

Colwellia arctica sp. nov., isolated from Arctic marine sediment

Original Paper

Abstract

Strain 435T, a catalase- and oxidase-positive, beige-pigmented, facultatively anaerobic and Gram-stain-negative marine bacterium, was isolated from marine sediment collected in the Arctic (8°21′629′′E 72°8′827′′N). The cells of the type strain are short- to curve-rods and able to grow at 4–25 °C, pH 6.0–9.0 and in the presence of 0.5–6.0 % (w/v) NaCl. It can reduce nitrate to nitrite. The predominant isoprenoid quinone was identified as Q-8 and the polar lipids are comprised of phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content is 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 435T belongs to the genus Colwellia. Strain 435T was found to exhibit 92.1–95.6 % 16S rRNA gene sequence similarities with other species of the genus Colwellia. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain 435T can be considered to represent a novel species, Colwellia arctica sp. nov., of the genus Colwellia. The type strain is 435T (=CICC 10860T = ATCC BAA-2609T).

Keywords

Colwellia arctica sp. nov. 16S rRNA gene Polyphasic taxonomy Marine sediment 

Notes

Acknowledgments

This work was financially supported by the Chinese Polar Environment Comprehensive Investigation and Assessment Program (CHINARE2012-03-05, CHINARE2013-03-05), Public Science and Technology Research Funds Projects of Ocean (201005032-2) and the National Natural Science Foundation of China (31370057).

Supplementary material

10482_2014_366_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)
10482_2014_366_MOESM2_ESM.docx (53 kb)
Supplementary material 2 (DOCX 53 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.College of Marine ScienceShandong University at WeihaiWeihaiChina
  2. 2.Key Laboratory of Marine Bioactive Substances, First Institute of OceanographySOAQingdaoChina
  3. 3.State Key Laboratory of Microbial TechnologyShandong UniversityJinanChina

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