Antonie van Leeuwenhoek

, Volume 107, Issue 2, pp 539–545 | Cite as

Deinococcusradioresistens sp. nov., a UV and gamma radiation-resistant bacterium isolated from mountain soil

  • Sathiyaraj Srinivasan
  • Jae-Jin Lee
  • Sang-Yong Lim
  • Min-Ho Joe
  • Seong-Hun Im
  • Myung Kyum Kim
Original Paper

Abstract

Two Gram-negative, non-motile, short rod-shaped bacterial strains, designated as 8AT and 28A, were isolated from Mount Deogyusan, Jeonbuk Province, South Korea. The isolates were analyzed by a polyphasic approach, revealing variations in their phenotypic characters but high DNA–DNA hybridisation values reciprocally, confirming that they belong to the same species. Both the isolates also showed a high resistance to UV compared with Deinococcus radiodurans, and a gamma-radiation resistance similar to other members of the genus Deinococcus. Phylogenetic analysis with the 16S rRNA gene sequences of closely related species indicated their similarities were below 97 %. Chemotaxonomic data showed the most abundant fatty acids to be C16:1ω7c and C16:0. The strains can be distinguished from closely related species by the production of esterase (C4) and α-galactosidase, and by their ability to assimilate l-alanine, l-histidine and N-acetyl-d-glucosamine. Based on the phenotypic, phylogenetic, and chemotaxonomic data, the isolates represent a novel species of the genus Deinococcus, for which the name Deinococcusradioresistens sp. nov. is proposed. The type strain is 8AT (KEMB 9004-109T = JCM 19777T), and a second strain is 28A (KEMB 9004-113 = JCM 19778).

Keywords

Deinococcaceae Deinococcus radioresistens Radiation-resistant Taxonomy 

Notes

Acknowledgments

This work was supported by a special research grant from Seoul Women’s University (2015).

Supplementary material

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Supplementary material 1 (PPT 693 kb)
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Supplementary material 2 (PPT 148 kb)
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Supplementary material 3 (PPT 147 kb)

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Sathiyaraj Srinivasan
    • 1
  • Jae-Jin Lee
    • 1
  • Sang-Yong Lim
    • 2
  • Min-Ho Joe
    • 2
  • Seong-Hun Im
    • 2
  • Myung Kyum Kim
    • 1
  1. 1.Department of Bio & Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulKorea
  2. 2.Radiation Research Division for BiotechnologyKorea Atomic Energy Research InstituteJeongeupKorea

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