Antonie van Leeuwenhoek

, Volume 107, Issue 2, pp 539–545 | Cite as

Deinococcusradioresistens sp. nov., a UV and gamma radiation-resistant bacterium isolated from mountain soil

  • Sathiyaraj Srinivasan
  • Jae-Jin Lee
  • Sang-Yong Lim
  • Min-Ho Joe
  • Seong-Hun Im
  • Myung Kyum Kim
Original Paper


Two Gram-negative, non-motile, short rod-shaped bacterial strains, designated as 8AT and 28A, were isolated from Mount Deogyusan, Jeonbuk Province, South Korea. The isolates were analyzed by a polyphasic approach, revealing variations in their phenotypic characters but high DNA–DNA hybridisation values reciprocally, confirming that they belong to the same species. Both the isolates also showed a high resistance to UV compared with Deinococcus radiodurans, and a gamma-radiation resistance similar to other members of the genus Deinococcus. Phylogenetic analysis with the 16S rRNA gene sequences of closely related species indicated their similarities were below 97 %. Chemotaxonomic data showed the most abundant fatty acids to be C16:1ω7c and C16:0. The strains can be distinguished from closely related species by the production of esterase (C4) and α-galactosidase, and by their ability to assimilate l-alanine, l-histidine and N-acetyl-d-glucosamine. Based on the phenotypic, phylogenetic, and chemotaxonomic data, the isolates represent a novel species of the genus Deinococcus, for which the name Deinococcusradioresistens sp. nov. is proposed. The type strain is 8AT (KEMB 9004-109T = JCM 19777T), and a second strain is 28A (KEMB 9004-113 = JCM 19778).


Deinococcaceae Deinococcus radioresistens Radiation-resistant Taxonomy 



This work was supported by a special research grant from Seoul Women’s University (2015).

Supplementary material

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Supplementary material 1 (PPT 693 kb)
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Supplementary material 3 (PPT 147 kb)


  1. Brooks BW, Murray RGE (1981) Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int J Syst Bacteriol 31:353–360CrossRefGoogle Scholar
  2. Cappuccino JG, Sherman N (2002) Microbiology: a laboratory manual, 6th edn. Pearson Education, Inc., San FranciscoGoogle Scholar
  3. Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P, Murray RNG, Costitow RN, Nester EW, Wood WA, Krieg NR, Phillips GH (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington, pp 21–33Google Scholar
  4. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 39:224–229Google Scholar
  5. Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  6. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specified tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  7. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  8. Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by high performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469CrossRefGoogle Scholar
  9. Im S, Song D, Joe M, Kim D, Park DH, Lim S (2013) Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents. Bioprocess Biosyst Eng 36:781–789PubMedCrossRefGoogle Scholar
  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721PubMedCrossRefGoogle Scholar
  11. Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  12. Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207CrossRefGoogle Scholar
  13. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  14. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  15. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol 4:406–425Google Scholar
  16. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc., NewarkGoogle Scholar
  17. Srinivasan S, Kim MK, Lim S, Joe M, Lee M (2012a) Deinococcus daejeonensis sp. nov., isolated from sludge in a sewage disposal plant. Int J Syst Evol Microbiol 62:1265–1270PubMedCrossRefGoogle Scholar
  18. Srinivasan S, Lee JJ, Lim S, Joe M, Kim MK (2012b) Deinococcus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 62:2844–2850PubMedCrossRefGoogle Scholar
  19. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849CrossRefGoogle Scholar
  20. Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRefGoogle Scholar
  22. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882CrossRefGoogle Scholar
  23. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  24. Weisburg WG, Barns SM, Pellerier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703PubMedCentralPubMedGoogle Scholar
  25. Yoo SH, Weon HY, Kim SJ, Kim YS, Kim BY, Kwon SW (2010) Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples. Int J Syst Evol Microbiol 60:1191–1195PubMedCrossRefGoogle Scholar
  26. Zhang YQ, Sun CH, Li WJ, Yu LY, Zhou JQ, Xu LH, Jiang CL (2007) Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. Int J Syst Evol Microbiol 57:370–375PubMedCrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Sathiyaraj Srinivasan
    • 1
  • Jae-Jin Lee
    • 1
  • Sang-Yong Lim
    • 2
  • Min-Ho Joe
    • 2
  • Seong-Hun Im
    • 2
  • Myung Kyum Kim
    • 1
  1. 1.Department of Bio & Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulKorea
  2. 2.Radiation Research Division for BiotechnologyKorea Atomic Energy Research InstituteJeongeupKorea

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