Antonie van Leeuwenhoek

, Volume 107, Issue 2, pp 443–451 | Cite as

Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

  • Byung-Ho Bang
  • Moon-Soo Rhee
  • Dong-Ho Chang
  • Doo-Sang Park
  • Byoung-Chan Kim
Original Paper

Abstract

A novel, Gram-stain positive, facultative anaerobic, non-motile and straight to curve rod shaped bacterium, strain LV19T was isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus, which was collected from Yeong-dong, Chuncheongbuk-do, South Korea. The colonies of the new isolate were convex, circular, cream white in color and 1–2 mm in diameter after 3 days incubation on Tryptic Soy Agar at 37 °C. Based on the 16S rRNA gene sequence similarity, the new isolate was most closely related to Erysipelothrix inopinata MF-EP02T, E. rhusiopathiae ATCC 19414 T and E. tonsillarum T-305T (94.8, 93.8 and 93.7 % similarity, respectively). Strain LV19T grew optimally at 37 °C, at pH 8.0 and in the presence of 0.5 % (w/v) NaCl. Oxidase activity and catalase activity were negative. The major cellular fatty acids (>10 %) were C18:2 cis-9,12 (28.9 %), C18:1 cis-9 (22.3 %), C16:0 (22.2 %) and C18:0 (18.5 %). The cell-wall hydrolysates contained ribose as a major sugar. Major polar lipids were phosphatidylglycerol and three unidentified glycolipids. No quinone was detected. The G+C content of the genomic DNA was 36.3 mol%. The levels of DNA–DNA relatedness between strain LV19T and all the reference strains were less than 20 %. On the basis of polyphasic evidence from this study, the isolate is considered to represent a novel species of the genus Erysipelothrix, for which the name Erysipelothrix larvae sp. nov. is proposed; the type strain is LV19T (=KCTC 33523T = DSM 28480T).

Keywords

Erysipelothrix larvae Trypoxylus dichotomus Larval gut microorganism Polyphasic taxonomy 

Abbreviations

PG

Phosphatidylglycerol

GL

Unidentified glycolipid

PL

Unidentified phospholipid,

AL

Unidentified aminolipid,

APGL

Unidentified aminophosphoglycolipid

TSA

Tryptic Soy Agar

rpm

Revolution per minute

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Byung-Ho Bang
    • 2
  • Moon-Soo Rhee
    • 1
  • Dong-Ho Chang
    • 1
  • Doo-Sang Park
    • 1
  • Byoung-Chan Kim
    • 1
  1. 1.Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC)Korea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonRepublic of Korea
  2. 2.Department of Food and Nutrition ScienceEulji UniversitySeongnam-siRepublic of Korea

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