Chryseobacterium polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Chryseobacterium
- 278 Downloads
A Gram-stain negative, rod-shaped and non-endospore forming bacterium, designated strain YG4-6T, was isolated from Polytrichastrum formosum collected from Gawalong glacier in Tibet, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain YG4-6T were identified as iso-C15:0 (29.3 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c as defined by MIDI, 23.5 %) and iso-C17:0 3-OH (16.5 %). The major polar lipids were found to consist of five unidentified aminolipids and three unidentified lipids. Strain YG4-6T was found to contain MK-6 as the dominant menaquinone and the G+C content of its genomic DNA was determined to be 37.3 mol%. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YG4-6T is affiliated to Chryseobacterium species, and its closest related species were Chryseobacterium aahli T68T (97.9 % sequence similarity), Chryseobacterium zeae JM-1085T (97.8 % sequence similarity), Chryseobacterium yeoncheonense DCY67T (97.6 % sequence similarity) and Chryseobacterium soldanellicola NBRC 100864T (97.2 % sequence similarity). However, the DNA–DNA relatedness values between these strains and strain YG4-6T were found to be clearly below 70 %. Based on the phylogenetic inference and phenotypic data, strain YG4-6T is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium polytrichastri sp. nov. is proposed. The type strain is YG4-6T (=CGMCC 1.12491T = DSM 26899T). An emended description of the genus Chryseobacterium is also proposed.
KeywordsChryseobacterium polytrichastri Moss Taxonomy
This work was funded by the Scientific Research Program of National Natural Science Foundation of China (No. 31100004 and No. 31470136).
- Breznak JA, Costilow RN (2007) Physicochemical factors in growth. In: Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 309–329Google Scholar
- Dong XZ, Cai MY (2001) Determination of biochemical properties. Manual for systematic identification of general bacteria. Science Press, Beijing, pp 370–398 (in Chinese)Google Scholar
- Kämpfer P, Vaneechoutte M, Lodders N, Baere TD, Avesani V, Janssens M et al (2009) Description of Chryseobacterium anthropi sp. nov. to accommodate clinical isolates biochemically similar to Kaistella koreensis and Chryseobacterium haifense, proposal to reclassify Kaistella koreensis asChryseobacterium koreense comb. nov. and emended description of the genus Chryseobacterium. Int J Syst Evol Microbiol 59:2421–2428PubMedCrossRefGoogle Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- Marmur J, Schildkraut CL, Doty P (1961) The reversible denaturation of DNA and its use in studies of nucleic acid homologies and the biological relatedness of microorganisms. J Chim Phys 58:945–955Google Scholar
- Montero-Calasanz M, Göker M, Rohde M, Spröer C, Schumann P, Busse HJ et al (2013) Chryseobacterium hispalense sp. nov., a plant growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery and emendation of the species Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol 63:4386–4395CrossRefGoogle Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. DE, MIDI Inc., NewarkGoogle Scholar
- Smibert RM, Kreg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654Google Scholar
- Tindall BJ, Sikorski J, Smibert RM, Kreig NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, 3rd edn. ASM Press, Washington, DC, pp 330–393Google Scholar
- Wu YF, Wu QL, Liu SJ (2013) Chryseobacterium taihuense sp nov., isolated from a eutrophic lake, and emended descriptions of the genus Chryseobacterium, Chryseobacterium taiwanense, Chryseobacterium jejuense and Chryseobacterium indoltheticum. Int J Syst Evol Microbiol 63:913–919PubMedCrossRefGoogle Scholar