Antonie van Leeuwenhoek

, Volume 107, Issue 2, pp 403–410 | Cite as

Chryseobacterium polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Chryseobacterium

Original Paper

Abstract

A Gram-stain negative, rod-shaped and non-endospore forming bacterium, designated strain YG4-6T, was isolated from Polytrichastrum formosum collected from Gawalong glacier in Tibet, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain YG4-6T were identified as iso-C15:0 (29.3 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c as defined by MIDI, 23.5 %) and iso-C17:0 3-OH (16.5 %). The major polar lipids were found to consist of five unidentified aminolipids and three unidentified lipids. Strain YG4-6T was found to contain MK-6 as the dominant menaquinone and the G+C content of its genomic DNA was determined to be 37.3 mol%. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YG4-6T is affiliated to Chryseobacterium species, and its closest related species were Chryseobacterium aahli T68T (97.9 % sequence similarity), Chryseobacterium zeae JM-1085T (97.8 % sequence similarity), Chryseobacterium yeoncheonense DCY67T (97.6 % sequence similarity) and Chryseobacterium soldanellicola NBRC 100864T (97.2 % sequence similarity). However, the DNA–DNA relatedness values between these strains and strain YG4-6T were found to be clearly below 70 %. Based on the phylogenetic inference and phenotypic data, strain YG4-6T is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium polytrichastri sp. nov. is proposed. The type strain is YG4-6T (=CGMCC 1.12491T = DSM 26899T). An emended description of the genus Chryseobacterium is also proposed.

Keywords

Chryseobacterium polytrichastri Moss Taxonomy 

Notes

Acknowledgment

This work was funded by the Scientific Research Program of National Natural Science Foundation of China (No. 31100004 and No. 31470136).

Supplementary material

10482_2014_338_MOESM1_ESM.docx (1.7 mb)
The phylogenetic trees made by neighbour-joining and maximum parsimony methods, transmission electron micrographs and the TLC of the total polar lipids of strain YG4-6T are available as supplementary figures with the online version of this paper. (DOCX 1697 kb)

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  1. 1.College of Life ScienceCapital Normal UniversityBeijingChina

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