Antonie van Leeuwenhoek

, Volume 107, Issue 2, pp 403–410 | Cite as

Chryseobacterium polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Chryseobacterium

Original Paper


A Gram-stain negative, rod-shaped and non-endospore forming bacterium, designated strain YG4-6T, was isolated from Polytrichastrum formosum collected from Gawalong glacier in Tibet, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain YG4-6T were identified as iso-C15:0 (29.3 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c as defined by MIDI, 23.5 %) and iso-C17:0 3-OH (16.5 %). The major polar lipids were found to consist of five unidentified aminolipids and three unidentified lipids. Strain YG4-6T was found to contain MK-6 as the dominant menaquinone and the G+C content of its genomic DNA was determined to be 37.3 mol%. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YG4-6T is affiliated to Chryseobacterium species, and its closest related species were Chryseobacterium aahli T68T (97.9 % sequence similarity), Chryseobacterium zeae JM-1085T (97.8 % sequence similarity), Chryseobacterium yeoncheonense DCY67T (97.6 % sequence similarity) and Chryseobacterium soldanellicola NBRC 100864T (97.2 % sequence similarity). However, the DNA–DNA relatedness values between these strains and strain YG4-6T were found to be clearly below 70 %. Based on the phylogenetic inference and phenotypic data, strain YG4-6T is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium polytrichastri sp. nov. is proposed. The type strain is YG4-6T (=CGMCC 1.12491T = DSM 26899T). An emended description of the genus Chryseobacterium is also proposed.


Chryseobacterium polytrichastri Moss Taxonomy 



This work was funded by the Scientific Research Program of National Natural Science Foundation of China (No. 31100004 and No. 31470136).

Supplementary material

10482_2014_338_MOESM1_ESM.docx (1.7 mb)
The phylogenetic trees made by neighbour-joining and maximum parsimony methods, transmission electron micrographs and the TLC of the total polar lipids of strain YG4-6T are available as supplementary figures with the online version of this paper. (DOCX 1697 kb)


  1. Behrendt U, Ulrich A, Schumann P (2008) Chryseobacterium gregarium sp. nov., isolated from decaying plant material. Int J Syst Evol Microbiol 58:1069–1074PubMedCrossRefGoogle Scholar
  2. Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070PubMedCrossRefGoogle Scholar
  3. Bernardet JF, Vancanneyt M, Matte-Tailliez O, Grisez L, Tailliez P, Bizet C et al (2005) Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals. Syst Appl Microbiol 28:640–660PubMedCrossRefGoogle Scholar
  4. Breznak JA, Costilow RN (2007) Physicochemical factors in growth. In: Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 309–329Google Scholar
  5. De Ley J (1970) Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:738–754PubMedCentralPubMedGoogle Scholar
  6. Dong XZ, Cai MY (2001) Determination of biochemical properties. Manual for systematic identification of general bacteria. Science Press, Beijing, pp 370–398 (in Chinese)Google Scholar
  7. Gillis M, Deley J, Decleene M (1970) Determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153PubMedCrossRefGoogle Scholar
  8. Ilardi P, Fernández J, Avendaňo-Herrera R (2009) Chryseobacterium piscicola sp. nov., isolated from diseased salmonid fish. Int J Syst Evol Microbiol 59:3001–3005PubMedCrossRefGoogle Scholar
  9. Im WT, Yang JF, Kim SY, Yi TH (2011) Chryseobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a rhus vernicifera-cultivated field. Int J Syst Evol Microbiol 61:1430–1435PubMedCrossRefGoogle Scholar
  10. Kämpfer P, Vaneechoutte M, Lodders N, Baere TD, Avesani V, Janssens M et al (2009) Description of Chryseobacterium anthropi sp. nov. to accommodate clinical isolates biochemically similar to Kaistella koreensis and Chryseobacterium haifense, proposal to reclassify Kaistella koreensis asChryseobacterium koreense comb. nov. and emended description of the genus Chryseobacterium. Int J Syst Evol Microbiol 59:2421–2428PubMedCrossRefGoogle Scholar
  11. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H et al (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721PubMedCrossRefGoogle Scholar
  12. Kämpfer P, Arun AB, Young CC, Chen WM, Sridhar KR, Rekha PD (2010) Chryseobacterium arthrosphaerae sp. nov., isolated from the faeces of the pill millipede Arthrosphaera magna Attems. Int J Syst Evol Microbiol 60:1765–1769CrossRefGoogle Scholar
  13. Kämpfer P, McInroy JA, Glaeser SP (2014) Chryseobacterium zeae sp. nov., Chryseobacterium arachidis sp. nov., and Chryseobacterium geocarposphaerae sp. nov., isolated from the rhizosphere environment. Antonie Van Leeuwenhoek 105:491–500CrossRefGoogle Scholar
  14. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
  15. Li YH, Liu QF, Liu Y, Zhu JN, Zhang Q (2011) Endophytic bacterial diversity in roots of Typha angustifolia L. in the constructed Beijing Cuihu Wetland (China). Res Microbiol 162:124–131PubMedCrossRefGoogle Scholar
  16. Loch TP, Faisal M (2014) Chryseobacterium aahli sp. nov., isolated from lake trout (Salvelinus namaycush) and brown trout (Salmo trutta), and emended descriptions of Chryseobacterium ginsenosidimutans and Chryseobacterium gregarium. Int J Syst Evol Microbiol 64:1573–1579PubMedCrossRefGoogle Scholar
  17. Marmur J, Doty P (1962) Determination of base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118PubMedCrossRefGoogle Scholar
  18. Marmur J, Schildkraut CL, Doty P (1961) The reversible denaturation of DNA and its use in studies of nucleic acid homologies and the biological relatedness of microorganisms. J Chim Phys 58:945–955Google Scholar
  19. Montero-Calasanz M, Göker M, Rohde M, Spröer C, Schumann P, Busse HJ et al (2013) Chryseobacterium hispalense sp. nov., a plant growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery and emendation of the species Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol 63:4386–4395CrossRefGoogle Scholar
  20. Park MS, Jung SR, Lee KH, Lee MS, Do JO, Kim SB et al (2006) Chryseobacterium soldanellicola sp. nov. and Chryseobacterium taeanense sp. nov., isolated from roots of sand-dune plants. Int J Syst Evol Microbiol 56:433–438PubMedCrossRefGoogle Scholar
  21. Park YJ, Son HM, Lee EH, Kim JH, Mavlonov GT, Choi KJ et al (2013) Chryseobacterium gwangjuense sp nov., isolated from soil. Int J Syst Evol Microbiol 63:4580–4585PubMedCrossRefGoogle Scholar
  22. Reasoner DJ, Geldreich EE (1985) A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7PubMedCentralPubMedGoogle Scholar
  23. Ruijssenaars HJ, Hartsmans S (2001) Plate screening methods for the detection of polysaccharase-producing microorganisms. Appl Microbiol Biotechnol 55:143–149PubMedCrossRefGoogle Scholar
  24. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  25. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. DE, MIDI Inc., NewarkGoogle Scholar
  26. Shen FT, Kämpfer P, Young CC, Lai WA, Arun AB (2005) Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55:1301–1304PubMedCrossRefGoogle Scholar
  27. Smibert RM, Kreg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654Google Scholar
  28. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P, Maiden MCJ et al (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047PubMedCrossRefGoogle Scholar
  29. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRefGoogle Scholar
  30. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCentralPubMedCrossRefGoogle Scholar
  31. Tindall BJ (1990a) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130CrossRefGoogle Scholar
  32. Tindall BJ (1990b) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Letts 66:199–202CrossRefGoogle Scholar
  33. Tindall BJ, Sikorski J, Smibert RM, Kreig NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, 3rd edn. ASM Press, Washington, DC, pp 330–393Google Scholar
  34. Van-An H, Kim YJ, Ngoc Lan N, Yang DC (2013) Chryseobacterium yeoncheonense sp nov., with ginsenoside converting activity isolated from soil of a ginseng field. Arch Microbiol 195:463–471CrossRefGoogle Scholar
  35. Vandamme P, Bernardet JF, Segers P, Kersters K, Holmes B (1994) New perspectives in the classification of the Flavobacteria—description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom rev. Int J Syst Bacteriol 44:827–831CrossRefGoogle Scholar
  36. Vaneechoutte M, Kämpfer P, De Baere T, Avesani V, Janssens M, Wauters G (2007) Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c. Int J Syst Evol Microbiol 57:2623–2628PubMedCrossRefGoogle Scholar
  37. Wu YF, Wu QL, Liu SJ (2013) Chryseobacterium taihuense sp nov., isolated from a eutrophic lake, and emended descriptions of the genus Chryseobacterium, Chryseobacterium taiwanense, Chryseobacterium jejuense and Chryseobacterium indoltheticum. Int J Syst Evol Microbiol 63:913–919PubMedCrossRefGoogle Scholar
  38. Yoon JH, Kang SJ, Oh TK (2007) Chryseobacterium daeguense sp nov., isolated from wastewater of a textile dye works. Int J Syst Evol Microbiol 57:1355–1359PubMedCrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  1. 1.College of Life ScienceCapital Normal UniversityBeijingChina

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