Aquibacillus salifodinae sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China
- 200 Downloads
A Gram-positive, rod-shaped, strictly aerobic bacterium, strain WSY08-1T, was isolated from a salt mine in Wensu county, Xinjiang province, China. Spherical to ellipsoidal endospores were observed to be formed in terminal swollen sporangia. Strain WSY08-1T was found to be able to grow at 20–45 °C (optimum 37 °C), 0–10 % (w/v) NaCl (optimum 4 %, w/v) and pH 6.0–9.0 (optimum 7.0). Catalase and oxidase activities were observed to be positive. The cell-wall peptidoglycan of strain WSY08-1T was found to contain meso-diaminopimelic acid. Menaquinone-7 (MK-7) was identified as the predominant isoprenoid quinone. The polar lipids were found to consist of phosphatidylglycerol, diphosphatidylglycerol, an unknown glycolipid, two unknown phospholipids and an unknown lipid. The major cellular fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. The DNA G+C content was determined to be 36.9 mol%. Analysis of the 16S rRNA gene sequence showed that strain WSY08-1T is closely related to Aquibacillus halophilus B6BT, Aquibacillus koreensis BH30097T and Aquibacillus albus YIM 93642T (97.6, 96.9 and 96.5 % similarity, respectively). The level of DNA–DNA relatedness between strains WSY08-1T and A. halophilus B6BT was 31.4 %. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain WSY08-1T is considered to represent a novel species in the genus Aquibacillus, for which the name Aquibacillus salifodinae sp. nov. is proposed. The type strain is WSY08-1T (=JCM 19761T = CGMCC 1.12849T).
KeywordsAquibacillus salifodinae sp. nov. Polyphasic taxonomy 16S rRNA gene Salt mine
This work was supported by grants from the National Natural Science Foundation of China (No. 31170001).
- Amoozegar MA, Bagheri M, Didari M, Mehrshad M, Schumann P, Spröer C, Sánchez-Porro C, Ventosa A (2014) Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov. Int J Syst Evol Microbiol 64:3616–3623PubMedCrossRefGoogle Scholar
- Dong XZ, Cai MY (2001) Determination of biochemical properties. In: Dong XZ, Cai MY (eds) Manual for the systematic identification of General Bacteria. Science Press, Beijing, pp 370–398 in ChineseGoogle Scholar
- Lee JS, Lim JM, Lee KC, Lee JC, Park YH, Kim CJ (2006) Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56:251–257PubMedCrossRefGoogle Scholar
- Logan JM, Berge O, Bishop AH, Busse HJ, De Vos P, Fritze D, Heyndrickx M, Kämpfer P, Rabinovitch L, Salkinoja-Salonen MS, Seldin L, Ventosa A (2009) Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121PubMedCrossRefGoogle Scholar
- Skerman VBD (1967) A guide to the identification of the genera of bacteria, 2nd edn. Williams & Wilkins, BaltimoreGoogle Scholar
- Xu XW, Wu YH, Zhou Z, Wang CS, Zhou YG, Zhang HB, Wang Y, Wu M (2007) Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov., and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. Int J Syst Evol Microbiol 57:1619–1624PubMedCrossRefGoogle Scholar
- Zhu XF (2011) Modern experimental technique of microbiology. Zhejiang University Press, Hangzhou English translationGoogle Scholar