Antonie van Leeuwenhoek

, Volume 106, Issue 6, pp 1167–1184 | Cite as

Ophiostomatoid fungi including two new fungal species associated with pine root-feeding beetles in northern Spain

  • Pedro RomónEmail author
  • Z. Wilhelm De Beer
  • Mercedes Fernández
  • Julio Diez
  • Brenda D. Wingfield
  • Michael J. Wingfield
Original Paper


Many bark beetles live in a symbiosis with ophiostomatoid fungi but very little is known regarding these fungi in Spain. In this study, we considered the fungi associated with nine bark beetle species and one weevil infesting two native tree species (Pinus sylvestris and Pinus nigra) and one non-native (Pinus radiata) in Cantabria (Northern Spain). This included examination of 239 bark beetles or their galleries. Isolations yielded a total of 110 cultures that included 11 fungal species (five species of Leptographium sensu lato including Leptographium absconditum sp. nov., five species of Ophiostoma sensu lato including Ophiostoma cantabriense sp. nov, and one species of Graphilbum). The most commonly encountered fungal associates of the bark beetles were Grosmannia olivacea, Leptographium procerum, and Ophiostoma canum. The aggressiveness of the collected fungal species was evaluated using inoculations on two-year-old P. radiata seedlings. Leptographium wingfieldii, Leptographium guttulatum, and Ophiostoma ips were the only species capable of causing significant lesions.


Ophiostoma Leptographium Root-feeding beetles Two new fungal species 



We acknowledge the University of Pretoria, the National Research Foundation of South Africa (NRF), the Department of Education, Universities and Research of Basque Government, members of Tree Protection Co-operative Programme (TPCP), and the NRF/DST Centre of Excellence in Tree Health Biotechnology (CTHB) for financial support. We also acknowledge the technical and logistical support of Ms. Milagros Vallejo of the Cantabria Government and Ms. Izette Greyling, Mr. Darryl Herron and Mr. Johan van der Linde of the Forestry and Agricultural Biotechnology Institute (FABI).


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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Pedro Romón
    • 1
    Email author
  • Z. Wilhelm De Beer
    • 2
  • Mercedes Fernández
    • 3
  • Julio Diez
    • 4
  • Brenda D. Wingfield
    • 1
  • Michael J. Wingfield
    • 2
  1. 1.Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.Department of Microbiology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  3. 3.Department of Agroforestry SciencesUniversity of ValladolidPalenciaSpain
  4. 4.Department of Plant Production and Forest ResourcesUniversity of ValladolidPalenciaSpain

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