Antonie van Leeuwenhoek

, Volume 106, Issue 6, pp 1147–1155 | Cite as

Bacillus cihuensis sp. nov., isolated from rhizosphere soil of a plant in the Cihu area of Taiwan

  • Bo Liu
  • Guo-Hong Liu
  • Cetin Sengonca
  • Peter Schumann
  • Ming-Kuang Wang
  • Jian-Yang Tang
  • Mei-Chun Chen
Original Paper

Abstract

A Gram-positive, moderately halotolerant, rod-shaped, spore forming bacterium, designated strain FJAT-14515T was isolated from a soil sample in Cihu area, Taoyuan County, Taiwan. The strain grew at 10–35 °C (optimum at 30 °C), pH 5.7–9.0 (optimum at pH 7.0) and at salinities of 0–5 % (w/v) NaCl (optimum at 1 % w/v). The diagnostic diamino acid of the peptidoglycan of the isolated strain was meso-diaminopimelic acid and major respiratory isoprenoid quinone was MK-7. Major cellular fatty acids were anteiso-C15:0 (40.6 %), iso-C15:0 (20.7 %) and the DNA G+C content of strain FJAT-14515T was 37.1 mol %. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-14515T belongs to the genus Bacillus, and was most closely related to the reference strains of Bacillus muralis DSM 16288T (97.6 %) and Bacillus simplex DSM 1321T (97.5 %). Levels of DNA–DNA relatedness between strain FJAT-14515T and the reference strains of B. muralis DSM 16288T and B. simplex DSM 1321T were 27.9 % ± 3.32 and 44.1 % ± 0.57, respectively. Therefore, on the basis of phenotypic, chemotaxonomic and genotypic properties, strain FJAT-14515T represents a novel species of the genus Bacillus, for which the name Bacillus cihuensis sp. nov. is proposed. The type strain is FJAT-14515T (=DSM 25969T = CGMCC 1.12697T).

Keywords

Bacillus cihuensis DNA–DNA relatedness Phylogenetic analysis Polyphasic taxonomy 

Notes

Acknowledgments

We thank Professor J. P. Euzéby for his suggestion on the spelling of the specific epithet. We thank also the Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, PR China, and the international cooperation project of Chinese Ministry of Science and Technology (2012DFA31120), Natural Science Foundation of China (NSFC) (31370059), 948 project of Chinese Ministry of Agriculture (2011-G25), 973 program earlier research project (2011CB111607) and project of agriculture science and technology achievement transformation (2010GB2C400220) for the supporting, respectively.

Supplementary material

10482_2014_284_MOESM1_ESM.docx (1 mb)
Supplementary material 1 (DOC 1061 kb)

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Bo Liu
    • 1
  • Guo-Hong Liu
    • 1
  • Cetin Sengonca
    • 2
  • Peter Schumann
    • 3
  • Ming-Kuang Wang
    • 4
  • Jian-Yang Tang
    • 1
  • Mei-Chun Chen
    • 1
  1. 1.Agricultural Bio-resource InstituteFujian Academy of Agricultural SciencesFuzhouChina
  2. 2.Institute of Crop Sciences and Resource Conservation (INRES)University of BonnBonnGermany
  3. 3.Leibniz Institute DSMZ-German Collection of Microorganisms and Cell CulturesBraunschweigGermany
  4. 4.Department of Agricultural ChemistryNational Taiwan UniversityTaipeiTaiwan

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