Antonie van Leeuwenhoek

, Volume 106, Issue 4, pp 733–742 | Cite as

Maribacter caenipelagi sp. nov., a member of the Flavobacteriaceae isolated from a tidal flat sediment of the Yellow Sea in Korea

Original Paper


A Gram-strain negative, rod-shaped and gliding bacterial strain, designated HD-44T, was isolated from a tidal flat sediment in the Korean peninsula. Strain HD-44T was found to grow optimally at pH 7.0–8.0, at 25° C and in the presence of 2–3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain HD-44T falls within the clade comprising the Maribacter species, clustering with the type strains of Maribacter aquivivus, Maribacter ulvicola, Maribacter stanieri, Maribacter dokdonensis, Maribacter orientalis, Maribacter forsetii, Maribacter arcticus and Maribacter sedimenticola, with which it exhibits 97.1–98.3 % sequence similarity values. Sequence similarities to the type strains of the other recognized Maribacter species are 95.6–96.8 %. Strain HD-44T was found to contain MK-6 as the predominant menaquinone and iso-C17:0 3-OH, iso-C15:1 G, iso-C15:0 and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) as the major fatty acids. The major polar lipids were identified as phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HD-44T was determined to be 37.6 mol% and its mean DNA–DNA relatedness values with M. aquivivus KCTC 12968T, M. ulvicola KCTC 12969T, M. stanieri KCTC 22023T, M. dokdonensis DSW-8T, M. orientalis KCTC 12967T, M. forsetii KT02ds18-6T, M. arcticus KCTC 22053T and M. sedimenticola KCTC 12966T were 27.7–8.0 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain HD-44T is distinguishable from recognized Maribacter species. On the basis of the data presented, strain HD-44T is proposed to represent a novel species of the genus Maribacter, for which the name Maribacter caenipelagi sp. nov. is proposed. The type strain is HD-44T (= KCTC 32549T = CECT 8455T).


Maribacter caenipelagi sp. nov. Bacteroidetes Flavobacteriaceae Tidal flat sediment 



This work was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK 21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

Supplementary material

10482_2014_243_MOESM1_ESM.doc (27 kb)
Supplementary material 1 (DOC 27 kb)


  1. Barbeyron T, Carpentier F, L’Haridon S, Schüler M, Michel G, Amann R (2008) Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 58:790–797PubMedCrossRefGoogle Scholar
  2. Barrow GI, Feltham RKA (1993) Cowan and Steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  3. Baumann P, Baumann L (1981) The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin, pp 1302–1331Google Scholar
  4. Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868PubMedGoogle Scholar
  5. Bruns A, Rohde M, Berthe-Corti L (2001) Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006PubMedCrossRefGoogle Scholar
  6. Cho KH, Hong SG, Cho HH, Lee YK, Chun J, Lee HK (2008) Maribacter arcticus sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 58:1300–1303PubMedCrossRefGoogle Scholar
  7. Cohen-Bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68CrossRefGoogle Scholar
  8. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721PubMedCrossRefGoogle Scholar
  10. Komagata K, Suzuki KI (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207CrossRefGoogle Scholar
  11. Lányí B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67CrossRefGoogle Scholar
  12. Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184PubMedCentralPubMedGoogle Scholar
  13. Lo N, Jin HM, Jeon CO (2013) Maribacter aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Maribacter. Int J Syst Evol Microbiol 63:3409–3414PubMedCrossRefGoogle Scholar
  14. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117CrossRefGoogle Scholar
  15. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  16. Nedashkovskaya OI, Kim SB, Han SK, Lysenko AM, Rohde M, Rhee MS, Frolova GM, Falsen E, Mikhailov VV, Bae KS (2004) Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov. Int J Syst Evol Microbiol 54:1017–1023PubMedCrossRefGoogle Scholar
  17. Nedashkovskaya OI, Vancanneyt M, De Vos P, Kim SB, Lee MS, Mikhailov VV (2007) Maribacter polysiphoniae sp. nov., isolated from a red alga. Int J Syst Evol Microbiol 57:2840–2843PubMedCrossRefGoogle Scholar
  18. Nedashkovskaya OI, Kim SB, Mikhailov VV (2010) Maribacter stanieri sp. nov., a marine bacterium of the family Flavobacteriaceae. Int J Syst Evol Microbiol 60:214–218PubMedCrossRefGoogle Scholar
  19. Reichenbach H (1992) The order Cytophagales. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes, vol 4, 2nd edn. Springer, New York, pp 3631–3675CrossRefGoogle Scholar
  20. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. Microbial ID, Inc., NewarkGoogle Scholar
  21. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849CrossRefGoogle Scholar
  22. Staley JT (1968) Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942PubMedCentralPubMedGoogle Scholar
  23. Tamaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  24. Thompson JD, Higgins DG, Gibson TJ (1994) Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCentralPubMedCrossRefGoogle Scholar
  25. Wayne LG, Brenner DJ, Colwell RR et al (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  26. Weerawongwiwat V, Kang H, Jung MY, Kim W (2013) Maribacter chungangensis sp. nov., isolated from a green seaweed, and emended descriptions of the genus Maribacter and Maribacter arcticus. Int J Syst Evol Microbiol 63:2553–2558PubMedCrossRefGoogle Scholar
  27. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY, Lee ST, Goodfellow M, Park YH (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505CrossRefGoogle Scholar
  28. Yoon JH, Lee ST, Park YH (1998) Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194PubMedCrossRefGoogle Scholar
  29. Yoon JH, Kang KH, Park YH (2003) Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454PubMedCrossRefGoogle Scholar
  30. Yoon JH, Kang SJ, Lee SY, Lee CH, Oh TK (2005) Maribacter dokdonensis sp. nov., isolated from sea water off a Korean island, Dokdo. Int J Syst Evol Microbiol 55:2051–2055PubMedCrossRefGoogle Scholar
  31. Zhang GI, Hwang CY, Kang SH, Cho BC (2009) Maribacter antarcticus sp. nov., a psychrophilic bacterium isolated from a culture of the Antarctic green alga Pyramimonas gelidicola. Int J Syst Evol Microbiol 59:1455–1459PubMedCrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Yong-Taek Jung
    • 1
    • 2
  • Jung-Sook Lee
    • 3
  • Jung-Hoon Yoon
    • 1
  1. 1.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonSouth Korea
  2. 2.University of Science and Technology (UST)DaejeonSouth Korea
  3. 3.Korea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonSouth Korea

Personalised recommendations