Antonie van Leeuwenhoek

, Volume 106, Issue 2, pp 357–363 | Cite as

Bacillus huizhouensis sp. nov., isolated from a paddy field soil

  • Jibing Li
  • Guiqin Yang
  • Min Wu
  • Yong Zhao
  • Shungui Zhou
Original Paper


A Gram-stain positive, facultative aerobic bacterium, designated as strain GSS03T, was isolated from a paddy field soil. The cells were observed to be endospore forming, rod-shaped and motile with flagella. The organism was found to grow optimally at 35 °C at pH 7.0 and in the presence of 1 % NaCl. The strain was classified as a novel taxon within the genus Bacillus on the basis of phenotypic and phylogenetic analyses. The closest phylogenetic relatives were identified as Bacillus psychrosaccharolyticus DSM 6T (97.61 %), Bacillus muralis DSM 16288T (97.55 %), Bacillus asahii JCM 12112T (97.48 %), Bacillus simplex DSM 1321T (97.48 %) and “Bacillus frigoritolerans” DSM 8801T (97.38 %). The menaquinone was identified as MK-7, the major cellular fatty acid was identified as anteiso-C15:0 and the major cellular polar lipids as phosphatidylethanolamine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and three unknown polar lipids. The DNA G+C content was determined to be 40.2 mol%. The DNA–DNA relatedness with the closest relatives was below 48 %. Therefore, on the basis of all the results, strain GSS03T is considered to represent a novel species within the genus Bacillus, for which the name Bacillus huizhouensis sp. nov. is proposed. The type strain is GSS03T (=KCTC 33172T =CCTCC AB 2013237T).


Bacillus huizhouensis sp. nov. Gram-staining positive Menaquinone Polar lipids 



This work was supported by the Team Project of Guangdong Natural Science Foundation (S2011030002882 and S2012030006114).

Supplementary material

10482_2014_208_MOESM1_ESM.doc (525 kb)
Supplementary material 1 (DOC 525 kb)


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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Jibing Li
    • 1
    • 2
  • Guiqin Yang
    • 2
  • Min Wu
    • 2
  • Yong Zhao
    • 1
  • Shungui Zhou
    • 2
  1. 1.College of Food Science and TechnologyShanghai Ocean UniversityShanghaiPeoples’s Republic of China
  2. 2.Guangdong Institute of Eco-Environmental and Soil SciencesGuangzhouPeoples’s Republic of China

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