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Antonie van Leeuwenhoek

, Volume 106, Issue 2, pp 211–217 | Cite as

Anhydrobiosis in yeast: is it possible to reach anhydrobiosis for yeast grown in conditions with severe oxygen limitation?

  • Linda Rozenfelde
  • Alexander Rapoport
Original Paper

Abstract

The yeast Saccharomyces cerevisiae was shown to be extremely sensitive to dehydration–rehydration treatments when stationary phase cells were subjected to conditions of severe oxygen limitation, unlike the same cells grown in aerobic conditions. The viability of dehydrated anaerobically grown yeast cells never exceeded 2 %. It was not possible to increase this viability using gradual rehydration of dry cells in water vapour, which usually strongly reduces damage to intracellular membranes. Specific pre-dehydration treatments significantly increased the resistance of anaerobic yeast to drying. Thus, incubation of cells with trehalose (100 mM), increased the viability of dehydrated cells after slow rehydration in water vapour to 30 %. Similarly, pre-incubation of cells in 1 M xylitol or glycerol enabled up to 50–60 % of cells to successfully enter a viable state of anhydrobiosis after subsequent rehydration. We presume that trehalose and sugar alcohols function mainly according to a water replacement hypothesis, as well as initiating various protective intracellular reactions.

Keywords

Anaerobic conditions Anhydrobiosis Cell resistance Dehydration Rehydration Saccharomyces cerevisiae 

Abbreviations

Tm

Phase transition temperature

APL

Area per lipid

NADH

Reduced form of nicotinamide adenine dinucleotide

Notes

Acknowledgments

This research was financially supported by the European Regional Development Fund Project (No. 2010/0288/2DP/2.1.1.1.0/10/APIA/VIAA/038) and the Latvian Science Council Project No. 372/2012.

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  1. 1.Laboratory of Cell Biology, Institute of Microbiology and BiotechnologyUniversity of LatviaRigaLatvia

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