Nocardia casuarinae sp. nov., an actinobacterial endophyte isolated from root nodules of Casuarina glauca
- 395 Downloads
An actinobacterium strain BMG51109a was isolated from surface sterilized root nodules of Casuarina glauca collected in Tunisia. The 16S rRNA gene sequence of strain BMG51109a showed most similarity (96.53–96.55 %) to the type strains of Nocardia transvalensis, N. aobensis and N. elegans. Chemotaxonomic analysis supported the assignment of the strain to Nocardia genus. The major menaquinone was MK-8(H4c) while the polar lipid profile contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, glycophospholipid, phosphatidylinositol, one uncharacterized phospholipid and three glycolipids. Whole-cell sugar analysis revealed the presence of meso-diaminopimelic acid, arabinose and galactose as diagnostic sugars, complemented by glucose, mannose and ribose. The major cellular fatty acids were tuberculostearic, oleic, palmitoleic and stearic acids. Physiological and biochemical tests showed that strain BMG51109a could be clearly distinguished from its closest phylogenetic neighbours. On the basis of these results, strain BMG51109aT (= DSM 45978T = CECT 8469T) is proposed as the type strain of the novel species Nocardia casuarinae sp. nov.
KeywordsNocardia casuarinae Casuarina Root nodule Sp. nov
The authors would like to gratefully acknowledge Prof. Manfred Rohde (Helmholz Centre for Infection Biology, Braunschweig, Germany) for providing the Scanning EM Photo in Fig. 2b. This research was supported by The Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, Tunisie.
- Boiron P, Provost F, Dupont B (1993) Laboratory methods for the diagnosis of nocardiosis. Institut Pasteur, Paris 180Google Scholar
- Chapin KC, Murray PR (1999) Stains. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds) Manual of clinical microbiology. American Society for Microbiology, Washington, DC, p 1678Google Scholar
- Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM (2009) The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37((Database issue)):D141–145. doi: 10.1093/nar/gkn879
- Goodfellow M (1998) Nocardia and related genera. Topley and Wilson’s microbiology and microbial infections, 9th edn. Edward Arnold, London, pp 463–489Google Scholar
- Goodfellow M, Maldonado LA (2010) Genus 1 Nocardia Trevisan 1889AL. In: Goodfellow M, Kämpfer P, Busse H-J, Trujillo ME, Suzuki K-I, Ludwig W, Whitman WB (eds) Bergey’s manual of systematic bacteriology: the actinobacteria Part A, vol 5, 2nd edn. Springer, New York, pp 376–419Google Scholar
- Hosoda J, Konomi T, Tani N, Aoki H, Imanaka H (1977) Isolation of new nocardicins from Nocardia uniformis subsp tsuyamanensis Agri. Biol Chem 41:2013–2020Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6Google Scholar