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Antonie van Leeuwenhoek

, Volume 105, Issue 5, pp 971–978 | Cite as

Hymenobacter qilianensis sp. nov., isolated from a subsurface sandstone sediment in the permafrost region of Qilian Mountains, China and emended description of the genus Hymenobacter

  • Lu Han
  • Shu-Jiao Wu
  • Chun-Yan Qin
  • You-Hai Zhu
  • Zhen-Quan Lu
  • Bing Xie
  • Jie LvEmail author
Original Paper

Abstract

A red–pink, Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain DK6-37 was isolated from the permafrost region of Qilian Mountains in northwest of China. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus Hymenobacter, with low sequence similarities (<97 %) to recognized Hymenobacter species. Optimum growth was observed at 28 °C, pH 7.0 and 0 % NaCl. The strain was found to contain MK-7 as the predominant menaquinone. The polar lipids were identified as phosphatidylethanolanmine, two unknown aminophospholipids, one unknown aminolipid and three unknown polar lipids. The major fatty acids were identified as summed feature 3 (C16:1 ω7c/C16:1 ω6c as defined by MIDI), summed feature 4 (anteiso-C17:1 B/iso-C17:1 I), C16:1 ω5c, iso-C17:0 3-OH, iso-C15:0 and C18:0. The DNA G + C content was determined to be 67.4 mol %. On the basis of the polyphasic evidence presented, it is proposed that strain DK6-37 represents a novel species of the genus Hymenobacter, for which the name Hymenobacter qilianensis sp. nov. is proposed. The type strain is DK6-37T (= CGMCC 1.12720T = JCM 19763T).

Keywords

Hymenobacter qilianensis sp. nov. Polyphasic taxonomy Subsurface sandstone sediment Qilian mountains permafrost 

Notes

Acknowledgments

This study was supported by the National Special Research Fund, China (Gas Hydrate Resource Exploration and Production Testing Project.Grant No. GZHL20110308, GZHL20110323).

Supplementary material

10482_2014_155_MOESM1_ESM.tif (15.4 mb)
Polar lipids profile of strain DK6-37T after separation by TLC. PE, phosphatidylethanolanmine; APL1, APL2, unknown aminophospholipids; AL, unknown aminolipid; L1-3, unknown polar lipid. Supplementary material 1 (TIFF 15766 kb)
10482_2014_155_MOESM2_ESM.tif (37.7 mb)
Neighbour-joining phylogenetic tree based on nearly complete 16S rRNA gene sequences showing the relationships between strain DK6-37T and strains of the genus Hymenobacter. Percentages at nodes are levels of bootstrap support (> 70 %) based on neighbour-joining analyses of 1000 resampled datasets. The sequence of Adhaeribacter aerophilus 6424S-25T was used as the outgroup. GenBank accession numbers are given in parentheses. Bar, 0.02 substitutions per nucleotide position. Supplementary material 2 (TIFF 38655 kb)
10482_2014_155_MOESM3_ESM.tif (26 mb)
Maximum-parsimony phylogenetictree based on nearly complete 16S rRNA gene sequences showing the relationships between strain DK6-37T and strains of the genus Hymenobacter. Percentages at nodes are levels of bootstrap support (>70 %) based on maximum-parsimony analyses of 1000 resampled datasets. The sequence of Adhaeribacter aerophilus 6424S-25T was used as the outgroup. GenBank accession numbers are given in parentheses. Supplementary material 3 (TIFF 26607 kb)

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Lu Han
    • 1
    • 2
  • Shu-Jiao Wu
    • 1
  • Chun-Yan Qin
    • 1
  • You-Hai Zhu
    • 3
  • Zhen-Quan Lu
    • 3
  • Bing Xie
    • 2
  • Jie Lv
    • 1
    Email author
  1. 1.College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingPeople’s Republic of China
  2. 2.College of Resources and Environmental ScienceEast China Normal UniversityShanghaiPeople’s Republic of China
  3. 3.Oil and Gas SurveyChina Geological SurveyBeijingPeople’s Republic of China

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