Rhodococcus jostii: a home for Rhodococcus strain RHA1
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The taxonomic position of Rhodococcus strain RHA1, an effective degrader of polychlorinated biphenyls with a large linear chromosome, was established using a polyphasic approach. The morphological and chemotaxonomic properties of the strain were typical of members of the genus Rhodococcus. The strain shared a high level of 16S rRNA sequence similarity (99.9 %) with the type strain of Rhodococcus jostii, a member of the Rhodococcus erythropolis subclade. The two strains shared a DNA:DNA relatedness value well above the cut-off point recommended for the circumscription of genomic species and had a broad range of phenotypic properties in common. The combination of genomic and phenotypic data show strain RHA1 to be a bona fide member of the species Rhodococcus jostii.
KeywordsRhodococcus strain RHA1 Polyphasic taxonomy Rhodococcus jostii
The authors are indebted to Lindsay Eltis and Bill Mohn of the University of British Columbia; Vancouver, for their helpful input into this study.
- Collins MD (1994) Isoprenoid quinones. In: Goodfellow M, O’Donnell AG (eds) Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 265–309Google Scholar
- Jones AL, Goodfellow M (2012) Genus Rhodococcus (Zopf 1891) emend Goodfellow, Alderson and Chun. In: Goodfellow M, Kämpfer P, Busse H-J, Trujillo ME, Suzuki K-I, Ludwig W, Whitman WB (eds) Bergey’s manual of systematic bacteriology, vol 5, 2nd edn., The Actinobacteria part ASpringer, New York, pp 437–464Google Scholar
- Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism, vol 3. Academic Press, New York, pp 21–132Google Scholar
- Kimbara K, Hashimoto T, Fukuda M, Koana T, Takagi M, Oshi M, Yano K (1989) Cloning and sequencing of two tandem genes involved in degradation of 2.3 dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS 102. J Bacteriol 171:2740–2747PubMedGoogle Scholar
- Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London pp, pp 173–199Google Scholar
- Minnikin DE, Hutchinson JG, Caldicott AB, Goodfellow M (1980) Thin-layer chromatography of methanolysates of mycolic-acid containing bacteria. J Chromatogr A188:221–233Google Scholar
- Schaal KP (1985) Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Wiley, Chichester, pp 359–381Google Scholar
- Whitman WB (2011) Intent of the nomenclatural code and recommendations about naming new species based on genomic sequences. The Bulletin of BISMiS 2:135–139Google Scholar