Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China
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A novel Gram-negative, rod shaped, motile, non-spore-forming, aerobic, brominated flame retardant hexabromocyclododecane-assimilating bacterium, designated strain GYP-17T, was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China. Colonies on 1/10 strength of marine Glycerol Enriched Medium plates were circular, dark-brown, 1–2 mm in diameter, and with regular margins. Growth occurred at 10–45 °C, 1–10 % (w/v) NaCl and pH of 7–9. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and one unidentified polar lipid. The major fatty acids were iso-C17:1ω9c/10-methyl-C16:0 (summed feature 9, 20.75 %), iso-C15:0 (17.70 %) and C16:0 (6.40 %). The major respiratory quinone was Q-8. The DNA G + C content of the type strain was 53.6 mol%. Phylogenetic analysis revealed that strain GYP-17T was a member of the genus Aliidiomarina and closely related to Aliidiomarina haloalkalitolerans with a 16S rDNA sequence similarity of 96.36 %. Results from the polyphasic taxonomy study support the conclusion that strain GYP-17T represents a novel Aliidiomarina species, for which the name Aliidiomarina sanyensis sp. nov. is proposed. The type strain of A. sanyensis is GYP-17T (=KCTC 32218T =LMG 27471T).
KeywordsAliidiomarina sanyensis 16S rDNA based phylogeny Chemotaxonomy
This research was supported by the National Basic Research Program of China (No. 2010CB833801), the National Natural Science Foundation of China (No. 41206136), Funds for marine renewable energy (GHME2011SW04), Public science and technology research funds projects of ocean (201005031-5), Guangdong Province and Chinese Academy of Science cooperation Foundation (2012B091100276), Provincial Collaborative Foundation Project of Guangdong (9351007002000001).
- Collins MD (1994) Isoprenoid quinones. In: Goodfellow M, O’Donnell AG (eds) Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 345–401Google Scholar
- Dong XZ, Cai MY (2001) Determinative manual for routine bacteriology. Scientific Press, BeijingGoogle Scholar
- Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376Google Scholar
- Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791Google Scholar
- Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DCGoogle Scholar
- Gutierrez T, Green DH, Nichols PD, Whitman WB, Semple KT, Aitken MD (2013) Polycyclovorans algicola gen. nov., sp. nov., an aromatic-hydrocarbon-degrading marine bacterium found associated with laboratory cultures of marine phytoplankton. Appl Environ Microbiol 79(1):205–214PubMedCrossRefGoogle Scholar
- Ivanova EP, Romanenko LA, Chun J, Matte MH, Matte GR, Mikhailov VV, Svetashev VI, Huq A, Maugel T, Colwell RR (2000) Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including description of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907PubMedCrossRefGoogle Scholar
- Ivanova EP, Flavier S, Christen R (2004) Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54:1773–1788PubMedCrossRefGoogle Scholar
- Kamekura M (1993) Lipids of extreme halophiles. In: Vreeland RH, Hochstein LI (eds) The biology of halophilic bacteria. CRC Press, Boca Raton, pp 135–161Google Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- Taborda M, Antunes A, Tiago I, Verssimo A (2009) Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina. Syst Appl Microbiol 32:371–378PubMedCrossRefGoogle Scholar