Antonie van Leeuwenhoek

, Volume 104, Issue 3, pp 301–307 | Cite as

Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria

  • Atika Meklat
  • Noureddine Bouras
  • Abdelghani Zitouni
  • Florence Mathieu
  • Ahmed Lebrihi
  • Peter Schumann
  • Cathrin Spröer
  • Hans-Peter Klenk
  • Nasserdine Sabaou
Original Paper


A novel halophilic actinomycete strain, H23T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23T had 16S rRNA gene sequence similarities ranging from 97.8 % (Actinopolyspora xinjiangensis TRM 40136T) to 94.8 % (Actinopolyspora mortivallis DSM 44261T). The strain grew optimally at pH 6.0–7.0, 28–32 °C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23T had MK-10 (H4) and MK-9 (H4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C17:0 (37.4 %), iso-C17:0 (14.8 %), iso-C15:0 (14.2 %), and iso-C16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23T (=DSM 45501T = CCUG 63368T = MTCC 11562T).


Actinopolysporarighensis sp. nov. halophilic actinomycete Algerian Sahara Polyphasic taxonomy 



The authors are grateful to Mohand Amokrane Meklat for providing soil sample from Oued Righ, Gabriele Pötter (DSMZ) for growing A. righensis cultures and for assistance with chemotaxonomical analyses.

Supplementary material

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Supplementary material 1 (PPTX 167 kb)
10482_2013_9948_MOESM2_ESM.doc (57 kb)
Supplementary material 2 (DOC 57 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Atika Meklat
    • 1
    • 2
  • Noureddine Bouras
    • 1
  • Abdelghani Zitouni
    • 1
  • Florence Mathieu
    • 3
  • Ahmed Lebrihi
    • 3
    • 4
  • Peter Schumann
    • 5
  • Cathrin Spröer
    • 5
  • Hans-Peter Klenk
    • 5
  • Nasserdine Sabaou
    • 1
  1. 1.Laboratoire de Biologie des Systèmes Microbiens (LBSM)Ecole Normale Supérieure de KoubaAlgerAlgeria
  2. 2.Département de Biologie, Faculté des Sciences Agronomiques, Vétérinaires et BiologiquesUniversité Saâd DahlebBlidaAlgeria
  3. 3.Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS)INPT-ENSAT, Université de ToulouseCastanet-TolosanFrance
  4. 4.Université Moulay IsmailMeknesMorocco
  5. 5.Leibniz Institute DSMZ—German Collection of Microorganisms and Cell CulturesBraunschweigGermany

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