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Antonie van Leeuwenhoek

, Volume 104, Issue 3, pp 301–307 | Cite as

Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria

  • Atika Meklat
  • Noureddine Bouras
  • Abdelghani Zitouni
  • Florence Mathieu
  • Ahmed Lebrihi
  • Peter Schumann
  • Cathrin Spröer
  • Hans-Peter Klenk
  • Nasserdine Sabaou
Original Paper

Abstract

A novel halophilic actinomycete strain, H23T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23T had 16S rRNA gene sequence similarities ranging from 97.8 % (Actinopolyspora xinjiangensis TRM 40136T) to 94.8 % (Actinopolyspora mortivallis DSM 44261T). The strain grew optimally at pH 6.0–7.0, 28–32 °C and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23T had MK-10 (H4) and MK-9 (H4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C17:0 (37.4 %), iso-C17:0 (14.8 %), iso-C15:0 (14.2 %), and iso-C16:0 (13.9 %). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23T (=DSM 45501T = CCUG 63368T = MTCC 11562T).

Keywords

Actinopolysporarighensis sp. nov. halophilic actinomycete Algerian Sahara Polyphasic taxonomy 

Notes

Acknowledgments

The authors are grateful to Mohand Amokrane Meklat for providing soil sample from Oued Righ, Gabriele Pötter (DSMZ) for growing A. righensis cultures and for assistance with chemotaxonomical analyses.

Supplementary material

10482_2013_9948_MOESM1_ESM.pptx (167 kb)
Supplementary material 1 (PPTX 167 kb)
10482_2013_9948_MOESM2_ESM.doc (57 kb)
Supplementary material 2 (DOC 57 kb)

References

  1. Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. J Appl Microbiol 12:421–423Google Scholar
  2. Chun J, Bae KS, Moon EY, Jung SO, Lee HK, Kim SJ (2000) Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50:1909–1913PubMedGoogle Scholar
  3. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  4. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  5. Fitch WM (1977) On the problem of discovering the most parsimonious tree. Am Nat 111:223–257CrossRefGoogle Scholar
  6. Gochnauer MB, Leppard GG, Komaratat P, Kates M, Novitsky T, Kushner DJ (1975) Isolation and characterization of Actinopolyspora halophila, gen. et sp. nov., an extremely halophilic actinomycete. Can J Microbiol 2:150–1511Google Scholar
  7. Gochnauer MB, Johnson KG, Kushner DJ (1989) Genus Actinopolyspora. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2398–2401Google Scholar
  8. Goodfellow M (1971) Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69:33–90PubMedCrossRefGoogle Scholar
  9. Gordon RE, Barnett DA (1977) Resistance to rifampicin and lysozyme of strains of some species of Mycobacterium and Nocardia as a taxonomic tool. Int J Syst Bacteriol 27:176–178CrossRefGoogle Scholar
  10. Gordon RE, Barnett DA, Handerhan JE, Pang CHN (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63CrossRefGoogle Scholar
  11. Guan TW, Liu Y, Zhao K, Xia ZF, Zhang XP, Zhang LL (2010) Actinopolyspora xinjiangensis sp. nov., a novel extremely halophilic actinomycete isolated from a salt lake in Xinjiang, China. Antonie Van Leeuwenhoek 98:447–453PubMedCrossRefGoogle Scholar
  12. Hozzein WN, Goodfellow M (2011) Actinopolyspora egyptensis sp. nov., a new halophilic actinomycete. Afr J Microbiol Res 5:100–105Google Scholar
  13. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism, vol 3. Academic Press, New York, pp 21–132Google Scholar
  14. Kelly, KL, Judd DB (1976) Color. Universal language and dictionary of names (National Bureau of Standards special publication 440). US Department of Commerce, WashingtonGoogle Scholar
  15. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence data-base with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721PubMedCrossRefGoogle Scholar
  16. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  17. Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367CrossRefGoogle Scholar
  18. Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 173–179Google Scholar
  19. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2. Bioinformatics 23:2947–2948PubMedCrossRefGoogle Scholar
  20. Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 34:435–444CrossRefGoogle Scholar
  21. Lechevalier MP, Bièvre C, Lechevalier HA (1977) Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260CrossRefGoogle Scholar
  22. Liu D, Coloe S, Baird R, Pedersen J (2000) Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol 38:471PubMedGoogle Scholar
  23. Marchal N, Bourdon JL, Richard CL (1987) Les milieux de culture pour l’isolement et l’identification biochimique des bactéries. Doin Press, ParisGoogle Scholar
  24. Meier-Kolthoff JP, Göker M, Spröer C, Klenk HP (2013) When should a DDH experiment be mandatory in microbial taxonomy? Arch Microbiol 195:413–418PubMedCrossRefGoogle Scholar
  25. Meklat A, Sabaou N, Zitouni A, Mathieu F, Lebrihi A (2011) Halophilic actinomycetes in Saharan soils of Algeria: isolation, taxonomy and antagonistic properties. Appl Environ Microbiol 77:6710–6714PubMedCrossRefGoogle Scholar
  26. Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Spröer C, Klenk HP, Sabaou N (2012) Actinopolyspora algeriensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil. Extremophiles 16:771–776PubMedCrossRefGoogle Scholar
  27. Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Spröer C, Klenk HP, Sabaou N (2013) Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria. Antonie Van Leeuwenhoek 103:771–776PubMedCrossRefGoogle Scholar
  28. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117CrossRefGoogle Scholar
  29. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188:221–233CrossRefGoogle Scholar
  30. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  31. Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092PubMedCrossRefGoogle Scholar
  32. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  33. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, technical note 101. Microbial ID, NewarkGoogle Scholar
  34. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340CrossRefGoogle Scholar
  35. Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155Google Scholar
  36. Tang SK, Wang Y, Klenk HP, Shi R, Lou K, Zhang YJ, Chen C, Ruan JS, Li WJ (2011) Actinopolyspora alba sp. nov. and Actinopolyspora erythraea sp. nov., isolated from a salt field, and reclassification of Actinopolyspora iraqiensis Ruan et al. 1994 as a heterotypic synonym of Saccharomonospora halophila. Int J Syst Evol Microbiol 61:1693–1698PubMedCrossRefGoogle Scholar
  37. Waksman SA (1961) Classification, identification, and descriptions of genera and species. In: The actinomycetes, vol 2. Williams & Wilkins, Baltimore, pp 331–332Google Scholar
  38. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) International committee on systematic bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematic. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  39. Yoshida M, Matsubara K, Kudo T, Horikoshi K (1991) Actinopolyspora mortivallis sp. nov., a moderately halophilic actinomycete. Int J Syst Bacteriol 41:15–20CrossRefGoogle Scholar
  40. Zhi XY, Li WJ, Stackebrandt E (2009) An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Atika Meklat
    • 1
    • 2
  • Noureddine Bouras
    • 1
  • Abdelghani Zitouni
    • 1
  • Florence Mathieu
    • 3
  • Ahmed Lebrihi
    • 3
    • 4
  • Peter Schumann
    • 5
  • Cathrin Spröer
    • 5
  • Hans-Peter Klenk
    • 5
  • Nasserdine Sabaou
    • 1
  1. 1.Laboratoire de Biologie des Systèmes Microbiens (LBSM)Ecole Normale Supérieure de KoubaAlgerAlgeria
  2. 2.Département de Biologie, Faculté des Sciences Agronomiques, Vétérinaires et BiologiquesUniversité Saâd DahlebBlidaAlgeria
  3. 3.Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS)INPT-ENSAT, Université de ToulouseCastanet-TolosanFrance
  4. 4.Université Moulay IsmailMeknesMorocco
  5. 5.Leibniz Institute DSMZ—German Collection of Microorganisms and Cell CulturesBraunschweigGermany

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