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Antonie van Leeuwenhoek

, Volume 104, Issue 2, pp 217–224 | Cite as

Aestuariihabitans beolgyonensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment

  • Jung-Hoon Yoon
  • Sooyeon Park
  • Yong-Taek Jung
Original Paper

Abstract

A Gram-negative, non-motile and rod-shaped bacterial strain, designated BB-MW15T, was isolated from a tidal flat of the southern coast of the Korean peninsula, and its taxonomic position was investigated by using a polyphasic taxonomic approach. Strain BB-MW15T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2–3 % (w/v) NaCl. Strain BB-MW15T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Marivita byunsanensis (96.8 %), Thalassobius maritimus (96.6 %), Sulfitobacter marinus (96.4 %) and Marinovum algicola (96.3 %). Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-MW15T forms an independent lineage within the evolutionary radiation encompassed by the Roseobacter clade of the class Alphaproteobacteria. Strain BB-MW15T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids detected in strain BB-MW15T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain BB-MW15T is 62.7 mol%. Based on phylogenetic, chemotaxonomic and other phenotypic characteristics, strain BB-MW15T represents a new genus and a new species within Alphaproteobacteria, for which the name Aestuariihabitans beolgyonensis gen. nov., sp. nov. is proposed. The type strain of A. beolgyonensis is BB-MW15T (= KCTC 32324T = CCUG 63829T).

Keywords

Aestuariihabitans beolgyonensis Novel genus Novel species Tidal flat 

Notes

Acknowledgments

This study was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK21 program from the Ministry of Science, ICT and Future Planning (MSIP) of the Republic of Korea.

Supplementary material

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Supplementary material 1 (PPT 145 kb)
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Supplementary material 2 (PPT 146 kb)
10482_2013_9940_MOESM3_ESM.ppt (162 kb)
Supplementary material 3 (PPT 162 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonSouth Korea

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