Aestuariihabitans beolgyonensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment
- 254 Downloads
A Gram-negative, non-motile and rod-shaped bacterial strain, designated BB-MW15T, was isolated from a tidal flat of the southern coast of the Korean peninsula, and its taxonomic position was investigated by using a polyphasic taxonomic approach. Strain BB-MW15T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2–3 % (w/v) NaCl. Strain BB-MW15T exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Marivita byunsanensis (96.8 %), Thalassobius maritimus (96.6 %), Sulfitobacter marinus (96.4 %) and Marinovum algicola (96.3 %). Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-MW15T forms an independent lineage within the evolutionary radiation encompassed by the Roseobacter clade of the class Alphaproteobacteria. Strain BB-MW15T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids detected in strain BB-MW15T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain BB-MW15T is 62.7 mol%. Based on phylogenetic, chemotaxonomic and other phenotypic characteristics, strain BB-MW15T represents a new genus and a new species within Alphaproteobacteria, for which the name Aestuariihabitans beolgyonensis gen. nov., sp. nov. is proposed. The type strain of A. beolgyonensis is BB-MW15T (= KCTC 32324T = CCUG 63829T).
KeywordsAestuariihabitans beolgyonensis Novel genus Novel species Tidal flat
This study was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK21 program from the Ministry of Science, ICT and Future Planning (MSIP) of the Republic of Korea.
- Euzéby JP (1997) List of Bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590-592 (List of Prokaryotic names with standing in nomenclature. http://www.bacterio.cict.fr/. Accessed 18 Jan 2013)
- Garrity GM, Bell JA, Lilburn T (2005) Order III. Rhodobacterales ord. nov. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol. 2, part C, 2nd edn. Springer, New York, p 161Google Scholar
- Martens T, Heidorn T, Pukall R, Simon M, Tindall BJ, Brinkhoff T (2006) Reclassification of Roseobacter gallaeciensis Ruiz- Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56:1293–1304PubMedCrossRefGoogle Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. Microbial ID, Inc, NewarkGoogle Scholar
- Uchino Y, Hirata A, Yokota A, Sugiyama J (1998) Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44:201–210PubMedCrossRefGoogle Scholar
- Yoon JH, Kang SJ, Lee MH, Oh TK (2007a) Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter. Int J Syst Evol Microbiol 57:1788–1792PubMedCrossRefGoogle Scholar
- Yoon JH, Kang SJ, Lee SY, Jung YT, Lee JS, Oh TK (2012b) Marivita hallyeonensis sp. nov., isolated from seawater, reclassification of Gaetbulicola byunsanensis as Marivita byunsanensis comb. nov. and emended description of the genus Marivita Hwang et al. 2009. Int J Syst Evol Microbiol 62:839–843PubMedCrossRefGoogle Scholar