Antonie van Leeuwenhoek

, Volume 103, Issue 5, pp 1023–1028 | Cite as

The teleomorph state of Candida deformans Langeron & Guerra and description of Yarrowia yakushimensis comb. nov.

  • Marizeth Groenewald
  • Maudy Th Smith
Original Paper


Yarrowia lipolytica is the only known species in the teleomorph (i.e. sexual) genus Yarrowia and has its anamorph (i.e. asexual state) classified in the genus Candida Berkhout as Candida lipolytica. This species can be found readily in nature, has significant industrial value and is important to the food and medical fields. Candida deformans was first considered a variety of C. lipolytica and was later listed as a synonym of this species. More recent studies based on sequence variation in the nuclear rRNA gene sequences suggested C. deformans to be a separate species with no known teleomorph linked to it. In this study we show that C. deformans strains, obtained from South Africa, can mate with strains present in the CBS yeast collection and produce a Yarrowia teleomorph, described here as Yarrowia deformans. Strains of Candida yakushimensis nom. inval., a species also belonging to the Yarrowia phylogenetic clade, were also studied and described as a novel species of Yarrowia.


Yarrowia deformans One fungus one name Forma asexualis 



Dr. T. Coutinho and late Prof. J.P. van der Walt are thanked for providing the South African strains.


  1. Benno Y, (2004) Microbe division (RIKEN BRC JCM). In: Annual report of the Riken Biological Research Center, RIKEN (in Japanese with English translation), Wako, pp 905–914Google Scholar
  2. Bigey F, Tuery K, Bougard D, Nicaud JM, Moulin G (2003) Identification of a triacylglycerol lipase gene family in Candida deformans: molecular cloning and functional expression. Yeast 20:233–248PubMedCrossRefGoogle Scholar
  3. de Hoog GS, Gerrits van den Ende AHG (1998) Molecular diagnostics of clinical strains of filamentous basidiomycetes. Mycoses 41:183–189PubMedCrossRefGoogle Scholar
  4. Groenewald M, Daniel H-M, Robert V, Poot GA, Smith MTh (2008) Polyphasic re-examination of Debaryomyces hansenii strains and re-instatement of D. hansenii, D. fabryi and D. subglobosus. Persoonia 21:17–27PubMedCrossRefGoogle Scholar
  5. Hawksworth DL, Crous PW, Redhead SA, Reynolds DR, Samson RA, Seifert KA, Taylor JW, Wingfield MJ et al (2011) The Amsterdam declaration on fungal nomenclature. IMA Fungus 2:105–112PubMedCrossRefGoogle Scholar
  6. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform (describes the FFT-NS-1, FFT-NS-2 and FFT-NS-i strategies). Nucleic Acids Res 30:3059–3066PubMedCrossRefGoogle Scholar
  7. Knutsen AK, Robert V, Poot GA, Epping W, Figge M, Holst-Jensen A, Skaar I, Smith MTh (2007) Polyphasic reexamination of Yarrowia lipolytica strains and the description of three new Candida species: C. oslonensis, C. alimentaria and C. hollandica. Int J Syst Evol Microbiol 57:2426–2435PubMedCrossRefGoogle Scholar
  8. Kurtzman CP (1998) Yarrowia van der Walt & von Arx. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. North-Holland, Amsterdam, pp 420–421CrossRefGoogle Scholar
  9. Kwon-Chung KJ, Varma A (2006) Do major species concepts support one, two or more species within Cryptococcus neoformans? FEMS Yeast Res 6:574–587PubMedCrossRefGoogle Scholar
  10. Kwon-Chung KJ, Bennett JE, Rhodes JC (1982) Taxonomic studies on Filobasidiella species and their anamorphs. Antonie Van Leeuwenhoek 48:25–38PubMedCrossRefGoogle Scholar
  11. Lachance M-A (2012) In defense of yeast sexual life cycles: the forma asexualis: an informal proposal. Yeast Newsl 61:24–25Google Scholar
  12. Lachance M-A, Rosa CA, Starmer WT, Schlag-Edler B, Barker JSF, Bowles JM (1998) Metschnikowia continentalis var. borealis, Metschnikowia continentalis var. continentalis, and Metschnikowia hibisci, new heterothallic haploid yeasts from ephemeral flowers and associated insects. Can J Microbiol 44:279–288Google Scholar
  13. Lachance M-A, Boekhout T, Scorzetti G, Fell JF, Kurtzman CP (2011) Candida Berkhout. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 987–1278CrossRefGoogle Scholar
  14. Langeron M, Guerra P (1938) Nouvelles recherches de zymologie médicale 2me Partie. Etudes monographiques sur les Mycotoruloidées. Le genre Candida Berkhout 1923. Ann Parasitol Hum Comp 16:481–525Google Scholar
  15. Limtong S, Youngmanitchai W, Kawasaki H, Seki T (2008) Candida phangngensis sp.nov., an anamorphic yeast species in the Yarrowia clade, isolated from water in mangrove forests in Phang-Nga Province, Thailand. Int J Syst Evol Microbiol 58:515–519PubMedCrossRefGoogle Scholar
  16. Meyer SA, Ahearn DG, Yarrow D (1984) Candida Berkhout. In: Kreger-van Rij NJW (ed) The Yeasts, A Taxonomic Study, 3rd edn. Elsevier, Amsterdam, pp 585–844Google Scholar
  17. Naumov GI, Naumova ES, Kondratieva VI, Bulat SA, Mironenko NV, Mendonca-Hagler LC, Hagler AN (1997) Genetic and molecular delineation of three sibling species in the Hansenula polymorpha complex. Syst Appl Microbiol 20:50–56CrossRefGoogle Scholar
  18. Péter G (2012) In support of the forma asexualis. Yeast Newsl 61:25–26Google Scholar
  19. Smith MTh, Robert V, Poot GA, Epping W, de Cock AWAM (2005) Taxonomy and phylogeny of the ascomycetous yeast genus Zygoascus, with proposal of Zygoascus meyerae sp. nov. and related anamorphic varieties. Int J Syst Evol Microbiol 55:1353–1363PubMedCrossRefGoogle Scholar
  20. Swofford DL (2003) PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, SunderlandGoogle Scholar
  21. Van Uden N, Buckley HR (1970) Candida Berkhout. In: Lodder J (ed) The yeasts, a taxonomic study, 2nd edn. North-Holland, Amsterdam, pp 893–1087Google Scholar
  22. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246PubMedGoogle Scholar
  23. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenics. In: Innis N, Gelfand D, Sninsky J, White T (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar
  24. Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts: a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.CBS-KNAW Fungal Biodiversity CentreUtrechtThe Netherlands

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