Antonie van Leeuwenhoek

, Volume 105, Issue 3, pp 541–550 | Cite as

Catching speciation in the act: Metschnikowia bowlesiae sp. nov., a yeast species found in nitidulid beetles of Hawaii and Belize

Original Paper
First Online:
Received:
Accepted:

Abstract

We describe the species Metschnikowia bowlesiae sp. nov. based on the recovery of six isolates from Hawaii and Belize. The species belongs to the Metschnikowia arizonensis subclade of the large-spored Metschnikowia clade. The isolates are haploid and heterothallic. Both Hawaiian strains had the mating type h + and the Belizean strains were h −. Paraphyletic species structures observed in some ribosomal DNA sequence analyses suggest that M. bowlesiae sp. nov. might represent an intermediate stage in a succession of peripatric speciation events from Metschnikowia dekortorum to Metschnikowia similis and might even hybridize with these species. The type of M. bowlesiae sp. nov. is strain UWOPS 04-243x5 (CBS 12940T, NRRL Y-63671) and the allotype is strain UWOPS 12-619.1 (CBS 12939A, NRRL Y-63670).

Keywords

Yeast Metschnikowia Peripatric speciation Paraphyletic species 
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Notes

Acknowledgments

This work was funded by the Natural Science and Engineering Council of Canada. We acknowledge the Granting of research or collecting permits by the Department of Land and Natural Resources of Hawaii, USA, and the Ministry of Natural Resources and the Environment of Belize. Field assistance from Jane Bowles and Curtis Ewing is gratefully acknowledged. The work in Belize was facilitated by Brock Fenton and Mark Howells. We thank Sheila Macfie for her critical reading of the revised manuscript.

Supplementary material

10482_2013_106_MOESM1_ESM.pdf (184 kb)
Online Resource 1 Phylogram of representatives of each ITS-D1/D2 phylotype (Fig. 2) of M. bowlesiae sp. nov., M. dekortorum, and M. similis with M. colocasiae as outgroup. The neighbour-joining analysis was performed on 1946 aligned positions in the ribosomal intergenic spacer (IGS). Gapped positions were deleted on a pair-wise basis. The scale bar shows the number of substitutions, which was the distance measure used in the analysis. Bootstraps from 1,000 replicates are shown. Supplementary material 1 (PDF 184 kb)
10482_2013_106_MOESM2_ESM.pdf (102 kb)
Online Resource 2 Phylogram showing the placement of M. bowlesiae sp. nov. in the M. arizonensis subclade. A maximum likelihood analysis was performed on 5439 non-gapped, aligned positions in the ribosomal gene cluster. The distances were fit to the general time reversible model. Bootstraps for 100 iterations are shown. A discrete Γ distribution was used to accommodate rate differences among sites (5 categories, Γ parameter = 0.05). The scale shows the patristic distance. Supplementary material 2 (PDF 102 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of BiologyUniversity of Western OntarioLondonCanada

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