Antonie van Leeuwenhoek

, Volume 105, Issue 3, pp 501–510 | Cite as

Bacillus bingmayongensis sp. nov., isolated from the pit soil of Emperor Qin’s Terra-cotta warriors in China

  • Bo Liu
  • Guo-Hong Liu
  • Gui-Ping Hu
  • Sengonca Cetin
  • Nai-Quan Lin
  • Jian-Yang Tang
  • Wei-Qi Tang
  • Ying-Zhi Lin
Original Paper


A Bacillus-like isolate, strain FJAT-13831T, isolated from the No. 1 pit soil of Emperor Qin’s Terra-cotta Warriors in Xi’an City, China, was studied to determine its taxonomic status. Dominant fatty acids of this organism included iso-C15:0, iso-C17:0, C16:0, iso-C13:0, anteiso-C15:0, and iso-C17:1ω5c. Comparative 16S rRNA gene sequence analysis confirmed the affiliation of this isolate to the genus Bacillus and indicated that it was closely related to Bacillus pseudomycoides DSM 12442T (99.72 % similarity). A phylogenetic analysis of the gyrB gene sequence similarities exhibited independent clustering of the isolate FJAT-13831T and showed 93.8 % (<95 %) sequence similarity with its closest phylogenetic neighbour B. pseudomycoides DSM 12442T. Separate standing of the strain FJAT-13831T was supported by a whole genome-based phylogenetic analysis with an average nucleotide identity value of 91.47 (<95 %) between isolate FJAT-13831T and B. pseudomycoides DSM 12442T and was consistent with the results of DNA–DNA hybridization (69.1 % relatedness). These findings support the conclusion that the isolate FJAT-13831T represents a novel species, for which the name Bacillus bingmayongensis sp. nov. is proposed. The type strain is FJAT-13831T (= CGMCC 1.12043T = DSM 25427T).


Bacillus bingmayongensis Emperor Qin’s Terra-cotta Warriors Polyphasic taxonomy Average nucleotide identity 



We are grateful to Dr. Jean P. Euzeby (Society for Systematic and Veterinary Bacteriology, France) for his advice on nomenclatural queries and Dr. Peter Schumann (DSMZ, Germany) for his contribution to the analysis of the cell wall composition of the strain FJAT-13831T. This work was supported by the National fund projects (31370059), by the National Key Project of Fundamental Scientific Research (“973” Program, No. 2011CB111607), by the project of Agriculture Science & Technology Achievement Transformation (No. 2010GB2C400220), by the program of International Science & Technology Cooperation (No. 2012DFA31120), by Agro-scientific Research in the Public Interest (No. 201303094), and by the national “948” project (No. 2011-G25) from the Chinese Ministry of Agriculture.

Supplementary material

10482_2013_102_MOESM1_ESM.docx (549 kb)
Supplementary material 1 (DOCX 548 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Bo Liu
    • 1
  • Guo-Hong Liu
    • 1
  • Gui-Ping Hu
    • 1
    • 2
  • Sengonca Cetin
    • 3
  • Nai-Quan Lin
    • 2
  • Jian-Yang Tang
    • 1
  • Wei-Qi Tang
    • 2
  • Ying-Zhi Lin
    • 1
  1. 1.Agricultural Bio-Resource InstituteFujian Academy of Agricultural SciencesFuzhouPeople’s Republic of China
  2. 2.Fujian Agricultural and Forest UniversityFuzhouPeople’s Republic of China
  3. 3.Institut fuer Nutzpflanzenwissenschaften und Ressourcenschutz (INRES), Phytomedizin-Entomologie und PflanzenschutzUniversitaet BonnBonnGermany

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