Antonie van Leeuwenhoek

, Volume 105, Issue 2, pp 325–333 | Cite as

Sphingobacterium pakistanensis sp. nov., a novel plant growth promoting rhizobacteria isolated from rhizosphere of Vigna mungo

  • Iftikhar AhmedEmail author
  • Muhammad Ehsan
  • Yeseul Sin
  • Jayoung Paek
  • Nauman Khalid
  • Rifat Hayat
  • Young H. ChangEmail author
Original Paper


The taxonomic status of a bacterium, strain NCCP-246T, isolated from rhizosphere of Vigna mungo, was determined using a polyphasic taxonomic approach. The strain NCCP-246T can grow at 16–37 °C (optimum 32 °C), at pH ranges of 6–8 (optimum growth occurs at pH 7) and in 0–4 % (w/v) NaCl. Phylogenetic analysis based upon on 16S rRNA gene sequence comparison revealed that strain NCCP-246T belonged to genus Sphingobacterium. Strain NCCP-246T showed highest similarity to the type strain of Sphingobacterium canadense CR11T (97.67 %) and less than 97 % with other species of the genus. The DNA–DNA relatedness value of strain NCCP-246T with S. canadense CR11T and Sphingobacterium thalpophilum JCM 21153T was 55 and 44.4 %, respectively. The chemotaxonomic data revealed the major menaquinone as MK-7 and dominant cellular fatty acids were summed feature 3 [C16:1 ω7c/C16:1 ω6c] (37.07 %), iso-C15:0 (28.03 %), C16:0 (11.85 %), C17:0 cyclo (8.84 %) and C14:0 (2.42 %). The G+C content of the strain was 39.2 mol%. On the basis of DNA–DNA hybridization, phylogenetic analyses, physiological and, biochemical data, strain NCCP-246T can be differentiated from the validly named members of genus Sphingobacterium and thus represents as a new species, for which the name, Sphingobacterium pakistanensis sp. nov. is proposed with the type strain NCCP-246T (= JCM18974 T = KCTC 23914T).


Sphingobacterium nifH gene Vigna mungo Antibiotic resistance P-solubilization 



We are thankful to Professor Dr. George Lazarovits from Southern Crop Protection and Food Research Centre, Canada and Dr. Moriya Ohkuma from Japan Collection of Microorganisms (JCM), Riken BioResource Centre, Tsukuba, Japan for providing the reference strains (Sphingobacterium canadense CR11T and S. thalpophilum JCM 21153T, respectively) for these experiments. This work was partially supported by PSDP Project “Research for Agricultural Development Program (RADP)” funded by Pakistan Agricultural Research Council (PARC). The partial financial support of KRIBB to the author “Muhammad Ehsan” in the training course at Korean Collection for Type Cultures, Biological Resources Centre, KRIBB under the umbrella of ACM forum is also gratefully acknowledged.

Supplementary material

10482_2013_77_MOESM1_ESM.docx (613 kb)
Supplementary material 1 (DOCX 613 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Iftikhar Ahmed
    • 1
    Email author
  • Muhammad Ehsan
    • 1
  • Yeseul Sin
    • 2
  • Jayoung Paek
    • 2
  • Nauman Khalid
    • 4
  • Rifat Hayat
    • 3
  • Young H. Chang
    • 2
    Email author
  1. 1.National Culture Collection of Pakistan (NCCP), National Institute for Genomics and Advanced Biotechnology (NIGAB)National Agricultural Research Center (NARC)IslamabadPakistan
  2. 2.Korean Collection for Type CulturesBiological Resources Centre, KRIBBTaejonRepublic of Korea
  3. 3.Department of Soil Science & SWCPMAS Arid Agriculture UniversityRawalpindiPakistan
  4. 4.Department of Global Agricultural SciencesThe University of TokyoTokyoJapan

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