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Antonie van Leeuwenhoek

, Volume 105, Issue 1, pp 81–87 | Cite as

Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment

  • Yong-Taek Jung
  • Sooyeon Park
  • Jung-Hoon YoonEmail author
Original Paper

Abstract

A Gram-negative, aerobic, non-motile, dark brown-coloured and rod-shaped bacterial strain, designated G-MB1T, was isolated from a tidal flat sediment of the South Sea, South Korea. Strain G-MB1T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain G-MB1T fell within the clade comprising Thalassomonas species, clustering with the type strains of Thalassomonas agarivorans, Thalassomonas loyana, Thalassomonas ganghwensis and Thalassomonas agariperforans, with which it exhibited 16S rRNA gene sequence similarity values of 96.0–96.9 %. The 16S rRNA gene sequence similarity values between strain G-MB1T and the type strains of the other Thalassomonas species were 94.6–95.1 %. Strain G-MB1T was found to contain Q-8 as the predominant ubiquinone and C16:0, C17:1 ω8c, C16:1 ω9c, C12:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as the major fatty acids. The major polar lipids of strain G-MB1T were phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. The DNA G+C content of strain G-MB1T was determined to be 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain G-MB1T is separated from other Thalassomonas species. On the basis of the data presented, strain G-MB1T is considered to represent a novel species of the genus Thalassomonas, for which the name Thalassomonas fusca sp. nov. is proposed. The type strain is G-MB1T (=KCTC 32499T = NBRC 109830T).

Keywords

Thalassomonasfusca Novel species Tidal flat Polyphasic taxonomy Dark brown colour 

Notes

Acknowledgments

This work was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

Supplementary material

10482_2013_55_MOESM1_ESM.ppt (187 kb)
Supplementary material 1 (PPT 187 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.University of Science and Technology (UST)TaejeonSouth Korea
  2. 2.Department of Food Science and BiotechnologySungkyunkwan UniversitySuwonSouth Korea

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