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Antonie van Leeuwenhoek

, Volume 103, Issue 4, pp 867–876 | Cite as

Lactobacillus ginsenosidimutans sp. nov., isolated from kimchi with the ability to transform ginsenosides

  • Hae-Min Jung
  • Qing-Mei Liu
  • Jin-Kwang Kim
  • Sung-Taik Lee
  • Sun-Chang Kim
  • Wan-Taek Im
Original Paper

Abstract

Biotransformation of ginsenosides was examined using lactic acid bacteria isolated from several kinds of kimchi. A Gram-positive, facultatively anaerobic, non-motile, non-spore-forming, and rod-shaped lactic acid bacterial strain, designated EMML 3041T, was determined to have ginsenoside-converting activity and its taxonomic position was investigated using a polyphasic approach. Strain EMML 3041T displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to F2 via gypenoside XVII, ginsenoside Rb2 to compound Y via compound O, ginsenoside Rc to compound Mc via compound Mc1, and ginsenoside Rd to ginsenoside F2. On the basis of the 16S rRNA gene sequence similarity, strain EMML 3041T was shown to belong to the genus Lactobacillus and is closely related to Lactobacillus versmoldensis KU-3T (98.3 % sequence similarity). Polyphasic taxonomy study confirmed that the strain EMML 3041T represents a novel species, for which the name Lactobacillus ginsenosidimutans sp. nov. is proposed, with EMML 3041T (=KACC 14527T = JCM 16719T) as the type strain.

Keywords

16S rRNA gene Polyphasic taxonomy Lactobacillus ginsenosidimutans Ginsenoside Biotransformation 

Notes

Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 program (No. PJ008193) of Rural Development Administration and by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967), and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Republic of Korea.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Hae-Min Jung
    • 1
  • Qing-Mei Liu
    • 2
  • Jin-Kwang Kim
    • 2
  • Sung-Taik Lee
    • 1
  • Sun-Chang Kim
    • 1
    • 2
  • Wan-Taek Im
    • 2
  1. 1.Department of Biological SciencesKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea
  2. 2.KI for Biocentury, Korea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea

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