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Antonie van Leeuwenhoek

, Volume 103, Issue 4, pp 789–796 | Cite as

Exiguobacterium himgiriensis sp. nov. a novel member of the genus Exiguobacterium, isolated from the Indian Himalayas

  • Nitin Kumar Singh
  • Revti Raichand
  • Ishwinder Kaur
  • Chandandeep Kaur
  • Siddhika Pareek
  • Shanmugam MayilrajEmail author
Original Paper

Abstract

The taxonomic position of an orange coloured bacterium, strain K22–26T isolated from a soil sample was studied using a polyphasic approach. The organism had phenotypic and chemotaxonomic properties consistent with its allocation into the genus Exiguobacterium. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain K22–26T belongs to the genus Exiguobacterium and was related to Exiguobacterium aurantiacum DSM 6208T (99.0 %) Exiguobacterium mexicanum DSM 16483T (98.6 %), Exiguobacterium aquaticum (98.6 %), Exiguobacterium aestuarii DSM 16306T (98.1 %), Exiguobacterium profundum DSM 17289T (98.1 %) and Exiguobacterium marinum DSM 16483T (97.9 %), whereas sequence similarity values with respect to other Exiguobacterium species with validly published names were between 92.5–94.0 %. The major polar lipids detected were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major menaquinone was determined to be MK-7 (83 %) whereas MK-8 (11 %) and MK-6 (6 %) occur in smaller amounts. The peptidoglycan of the strain was found to contain l-lysine as the diagnostic diamino acid. The major fatty acids detected were iso C13:0 (11.2 %), anteiso C13:0 (15.4 %), iso C15:0 (13.2 %) and iso C17:0 (16.1 %). However, analysis of the DNA–DNA relatedness confirmed that strain K22–26T belongs to a novel species. The G + C content of the strain K22–26T was determined to be 50.1 mol %. The novel strain was distinguished from closely related type species of the genus Exiguobacterium using DNA–DNA relatedness and phenotypic data. Based on these differences, the strain K22–26T should be classified as a novel species of the genus Exiguobacterium, for which the name Exiguobacterium himgiriensis sp. nov. strain K22–26T (= MTCC 7628T = JCM 14260T) is proposed.

Keywords

16S rRNA gene sequence DNA–DNA hybridization Fatty acid 

Notes

Acknowledgments

We thank Mr. Malkit Singh for his excellent technical assistance. This work was supported by Council of Scientific and Industrial Research (CSIR-network project NWP-006) Government of India. This is IMTECH communication number 094/2011.

Supplementary material

10482_2012_9861_MOESM1_ESM.doc (1.4 mb)
Supplementary material 1 (DOC 1480 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Nitin Kumar Singh
    • 2
  • Revti Raichand
    • 2
  • Ishwinder Kaur
    • 2
  • Chandandeep Kaur
    • 2
  • Siddhika Pareek
    • 2
  • Shanmugam Mayilraj
    • 1
    Email author
  1. 1.Microbial Type Culture Collection & Gene Bank (MTCC), Institute of Microbial Technology (IMTECH)ChandigarhIndia
  2. 2.A Constituent Laboratory of Council of Scientific and Industrial Research (CSIR)ChandigarhIndia

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