Isolation and characterization of a novel violacein-like pigment producing psychrotrophic bacterial species Janthinobacterium svalbardensis sp. nov
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A bacterial strain designated JA-1, related to Janthinobacterium lividum, was isolated from glacier ice samples from the island Spitsbergen in the Arctic. The strain was tested for phenotypic traits and the most prominent appeared to be the dark red brown to black pigmentation different from the violet pigment of Janthinobacterium, Chromobacterium and Iodobacter. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridization tests showed that strain JA-1 belongs to the genus Janthinobacterium but represents a novel lineage distinct from the two known species of this genus, J. lividum and Janthinobacterium agaricidamnosum. The DNA G + C content of strain JA-1 was determined to be 62.3 mol %. The isolate is a psychrotrophic Gram negative bacterium, rod-shaped with rounded ends, containing intracellular inclusions and one polar flagellum. On the basis of the presented results strain JA-1 is proposed as the type strain of a novel species of the genus Janthinobacterium, for which the name Janthinobacterium svalbardensis sp. nov. is proposed (JA-1T = DSM 25734, ZIM B637).
KeywordsPsychrotrophic bacterium Janthinobacterium svalbardensis sp. nov. Violacein-like pigment
We would like to express our gratitude to Prof. Dr. Nina Gunde Cimerman for the arctic glacier ice samples.
- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidmen JG, Smith JA, Struhl K (eds) (1992) Current protocols in molecular biology, vol 1. John Wiley and Sons, New YorkGoogle Scholar
- Bozzola JJ, Russell LD (1999) Electron Microscopy, 2nd edn. Jones and Bartlett Publishers, SudburyGoogle Scholar
- CLSI (2007) Performance standards for antimicrobial disk susceptibility testing: seventeenth international supplement M100–S17. CLSI, WayneGoogle Scholar
- Eisenberg J (1891) Bacteriologische Diagnostik Hilfstabellen zum Gebrauche beim Praktischen Arbeiten 3 Aufl. Leopold Voss, HamburgGoogle Scholar
- Logan NA, Moss MO (1992) Identification of Chromobacterium, Janthinobacterium and Iodobacter species. In: JonesBoard Skinner (ed) Identification methods in Applied and Environmental Microbiology. The Society for Applied Bacteriology Technical Series Blackwell Scientific Publications, OxfordGoogle Scholar
- Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar BA, Ali T, Steppi S, Jobb G, Förster W, Brettske I, Gerber S, Ginhart AW, Gross O, Grumann S, Hermann S, Jost R, König A, Liss T, Lüßmann R, May M, Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, Bode A, Schleifer K-H (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371PubMedCrossRefGoogle Scholar
- Moss MO, Ryall C (1981) The genus Chromobacterium. In: Starr, Stolp, Trüper, Balows and Schlegel (eds) The Prokaryotes, A Handbook on Habitats, Isolation, and Identification of Bacteria. Springer Verlag, BerlinGoogle Scholar
- Sneath PHA (1984) Genus Janthinobacterium. In: Krieg NR, Holt JG (eds) Bergey’s manual of systematic bacteriology. The Williams & Wilkins Co., Baltimore, pp 376–377Google Scholar
- Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the Ad Hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37(4):463–464Google Scholar
- Weisburg WG, Barms SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173(697–7):03Google Scholar