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Antonie van Leeuwenhoek

, Volume 103, Issue 4, pp 763–769 | Cite as

Isolation and characterization of a novel violacein-like pigment producing psychrotrophic bacterial species Janthinobacterium svalbardensis sp. nov

  • Jerneja Ambrožič Avguštin
  • Darja Žgur Bertok
  • Rok Kostanjšek
  • Gorazd Avguštin
Original Paper

Abstract

A bacterial strain designated JA-1, related to Janthinobacterium lividum, was isolated from glacier ice samples from the island Spitsbergen in the Arctic. The strain was tested for phenotypic traits and the most prominent appeared to be the dark red brown to black pigmentation different from the violet pigment of Janthinobacterium, Chromobacterium and Iodobacter. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridization tests showed that strain JA-1 belongs to the genus Janthinobacterium but represents a novel lineage distinct from the two known species of this genus, J. lividum and Janthinobacterium agaricidamnosum. The DNA G + C content of strain JA-1 was determined to be 62.3 mol %. The isolate is a psychrotrophic Gram negative bacterium, rod-shaped with rounded ends, containing intracellular inclusions and one polar flagellum. On the basis of the presented results strain JA-1 is proposed as the type strain of a novel species of the genus Janthinobacterium, for which the name Janthinobacterium svalbardensis sp. nov. is proposed (JA-1T = DSM 25734, ZIM B637).

Keywords

Psychrotrophic bacterium Janthinobacterium svalbardensis sp. nov. Violacein-like pigment 

Notes

Acknowledgments

We would like to express our gratitude to Prof. Dr. Nina Gunde Cimerman for the arctic glacier ice samples.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Jerneja Ambrožič Avguštin
    • 1
  • Darja Žgur Bertok
    • 1
  • Rok Kostanjšek
    • 2
  • Gorazd Avguštin
    • 3
  1. 1.Department of Biology, Chair for Molecular Genetics and Biology of MicroorganismsUniversity of Ljubljana, Biotechnical FacultyLjubljanaSlovenia
  2. 2.Department of Biology, Chair of ZoologyUniversity of Ljubljana, Biotechnical FacultyLjubljanaSlovenia
  3. 3.Animal Science Department, Chair for Microbiology and Microbial BiotechnologyUniversity of Ljubljana, Biotechnical FacultyDomžaleSlovenia

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