Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 647–653 | Cite as

Paenibacillus brassicae sp. nov., isolated from cabbage rhizosphere in Beijing, China

  • Miao Gao
  • Hui Yang
  • Ji Zhao
  • Jun Liu
  • Yan-hua Sun
  • Yu-jiong Wang
  • Jian-guang Sun
Original Paper


A novel Gram-positive, rod-shaped, motile, spore-forming, nitrogen-fixing bacterium, designated strain 112T, was isolated from cabbage rhizosphere in Beijing, China. The strain was found to grow at 10–40 °C and pH 4–11, with an optimum of 30 °C and pH 7.0, respectively. Phylogenetic analysis based on a fragment of the full-length 16S rRNA gene sequence revealed that strain 112T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarities were found between strain 112T, Paenibacillus sabinae DSM 17841T (97.82 %) and Paenibacillus forsythiae DSM 17842T (97.22 %). However, the DNA–DNA hybridization values between strain 112T and the type strains of these two species were 10.36 and 6.28 %, respectively. The predominant menaquinone was found to be menaquinone 7 (MK-7). The major fatty acids were determined to be anteiso-C15:0 and C16:0. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminophospholipids. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The DNA G+C content was determined to be 55.4 mol%. On the basis of its phenotypic characteristics, 16S rRNA gene sequence analysis and the value of DNA–DNA hybridization, strain 112T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus brassicae sp. nov. is proposed. The type strain is 112T (= ACCC 01125T = DSM 24983T).


Paenibacillus brassicae sp. nov. Nitrogen-fixation Cabbage rhizosphere 



This research was supported by the National Natural Science Foundation of China (No.30970083), and Special Fund for Agro-scientific Research in the Public Interest (201203045).

Supplementary material

10482_2012_9849_MOESM1_ESM.docx (80 kb)
Supplementary material 1 (DOCX 80 kb)


  1. Ash C, Priest FG, Collins MD (1993) Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 64:253–260PubMedCrossRefGoogle Scholar
  2. Atlas RM (1993) Handbook of microbiological media. In: Parks LC, CRC, Boca RatonGoogle Scholar
  3. Beneduzi A, Costa PB, Parma M, Melo IS, Bodanese-Zanettini MH, Passaglia LM (2010) Paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Triticum aestivum. Int J Syst Evol Microbiol 60:128–133PubMedCrossRefGoogle Scholar
  4. Berge O, Guinebretiere MH, Achouak W, Normand P, Heulin T (2002) Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52:607–616PubMedGoogle Scholar
  5. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261Google Scholar
  6. Collins M, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Microbiol 48:459–470CrossRefGoogle Scholar
  7. Elo S, Suominen I, Kampfer P, Juhanoja J, Salkinoja-Salonen M, Haahtela K (2001) Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51:535–545PubMedGoogle Scholar
  8. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  9. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  10. Forbes L (1981) Rapid flagella stain. J Clin Microbiol 13:807–809PubMedGoogle Scholar
  11. Gregersen T (1978) Rapid method for distinction of Gram-negative from Gram-positive bacteria. Appl Microbiol Biot 5:123–127CrossRefGoogle Scholar
  12. Harrigan W, McCance M (1976) Laboratory methods in foods and dairy microbiology. Academic, London, pp 66–81Google Scholar
  13. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322CrossRefGoogle Scholar
  14. Hong YY, Ma YC, Zhou YG, Gao F, Liu HC, Chen SF (2009) Paenibacillus sonchi sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Sonchus oleraceus. Int J Syst Evol Microbiol 59:2656–2661PubMedCrossRefGoogle Scholar
  15. Jiang RB, Ning GZ (2001) List of agricultural cultures of China. China Agricultural Scientech, BeijingGoogle Scholar
  16. Jin HJ, Lv J, Chen SF (2010) Paenibacillus sophorae sp. nov., a novel nitrogen-fixing species isolated from the rhizosphere of Sophora japonica. Int J Syst Evol Microbiol 61:767–771PubMedCrossRefGoogle Scholar
  17. Ma YC, Xia ZQ, Liu XM, Chen SF (2007a) Paenibacillus sabinae sp. nov., a nitrogen-fixing species isolated from the rhizosphere soils of shrubs. Int J Syst Evol Microbiol 57:6–11PubMedCrossRefGoogle Scholar
  18. Ma YC, Zhang J, Chen SF (2007b) Paenibacillus zanthoxyli sp. nov., a novel nitrogen-fixing species isolated from the rhizosphere of Zanthoxylum simulans. Int J Syst Evol Microbiol 57:873–877PubMedCrossRefGoogle Scholar
  19. Minnikin D, O’Donnell A, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett J (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  20. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  21. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc., NewarkGoogle Scholar
  22. Smibert RM, Krieg NR (1994) Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR, Manual of methods for general and microbiology, American Society for Microbiology, Washington, pp 607–654Google Scholar
  23. Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  24. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar
  25. Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A (1982) Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Appl Microbiol 128:1959–1968Google Scholar
  26. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464Google Scholar
  27. Weaver PF, Wall JD, Gest H (1975) Characterization of Rhodopseudomonas capsulata. Arch Microbiol 105:207–216PubMedCrossRefGoogle Scholar
  28. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703PubMedGoogle Scholar
  29. Yoon J, Kim H, Kim S, Kim H, Kim W, Lee S, Goodfellow M, Park Y (1996) Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Evol Microbiol 46:502–505Google Scholar
  30. Ziemke F, Hofle M, Lalucat J, Rossello-Mora R (1998) Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Miao Gao
    • 1
  • Hui Yang
    • 2
  • Ji Zhao
    • 3
  • Jun Liu
    • 3
  • Yan-hua Sun
    • 3
  • Yu-jiong Wang
    • 2
  • Jian-guang Sun
    • 1
  1. 1.Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western ChinaSchool of Life Science, Ningxia UniversityYinchuanPeople’s Republic of China
  3. 3.School of Life Science, Neimenggu UniversityHuhehaotePeople’s Republic of China

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