Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 559–565 | Cite as

Rhizobium qilianshanense sp. nov., a novel species isolated from root nodule of Oxytropis ochrocephala Bunge in China

  • Lin Xu
  • Yong Zhang
  • Zheng Shan Deng
  • Liang Zhao
  • Xiu Li Wei
  • Ge Hong Wei
Original Paper


During a study of the diversity and phylogeny of rhizobia isolated from root nodules of Oxytropis ochrocephala grown in the northwest of China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. These strains have identical 16S rRNA gene sequences, which showed a mean similarity of 94.4 % with the most closely related species, Rhizobium oryzae. Analysis of recA and glnA sequences showed that these strains have less than 88.1 and 88.7 % similarity with the defined species of Rhizobium, respectively. The genetic diversity revealed by ERIC-PCR fingerprinting indicated that the isolates correspond to different strains. Strain CCNWQLS01T contains Q-10 as the predominant ubiquinone. The major fatty acids were identified as feature 8 (C18: 1ω7c and/or C18: 1ω6c; 67.2 %). Therefore, a novel species Rhizobium qilianshanense sp. nov. is proposed, and CCNWQLS01T (= ACCC 05747T = JCM 18337T) is designated as the type strain.


Rhizobium Novel species Oxytropis ochrocephala China 



This work was supported by projects from National Science Foundation of China (31125007, 30970003), and the 863 Project of China (2012AA100602). We would like to express our gratitude to Professor Ruibo Jiang and Xiaoxia Zhang (Agricultural Cultural Collection of China, Chinese Academy of Agricultural Sciences, Beijing, China) for providing the type strains.

Supplementary material

10482_2012_9840_MOESM1_ESM.ppt (80 kb)
Fig. S1 Comparison of partial recA sequences. Phylogenetic tree was constructed by the neighbor-joining method from Jukes-Cantor distance matrices of the sequences. Bootstrap percentages above 50 % are indicated for phylogenies constructed by neighbor-joining method (PPT 79 kb)
10482_2012_9840_MOESM2_ESM.ppt (61 kb)
Fig. S2 Comparison of partial glnA sequences. Phylogenetic tree was constructed by the neighbor-joining method from Jukes-Cantor distance matrices of the sequences. Bootstrap percentages above 50 % are indicated for phylogenies constructed by neighbor-joining method (PPT 61 kb)
10482_2012_9840_MOESM3_ESM.ppt (63 kb)
Fig. S3 Comparison of partial nodA sequences. Phylogenetic tree was constructed by the neighbor-joining method from Jukes-Cantor distance matrices of the sequences. Bootstrap percentages above 50 % are indicated for phylogenies constructed by neighbor-joining method (PPT 63 kb)
10482_2012_9840_MOESM4_ESM.ppt (104 kb)
Fig. S4 ERIC-PCR profiles showing the relationship among Rhizobium qilianshanense sp. nov. Lanes: 1, CCNWQLS21; 2, CCNWQLS01T; 3, CCNWQLS97; 4, CCNWQLS111. Lane M, molecular mass marker (PPT 103 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Lin Xu
    • 1
    • 2
  • Yong Zhang
    • 2
  • Zheng Shan Deng
    • 1
  • Liang Zhao
    • 1
  • Xiu Li Wei
    • 1
  • Ge Hong Wei
    • 1
  1. 1.State Key Laboratory of Soil Erosion and Dryland Farming On the Loess Plateau, College of Life SciencesNorthwest A&F UniversityYanglingChina
  2. 2.Department of Life Science and EngineeringHexi UniversityZhangyeChina

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