Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 503–512 | Cite as

Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood

  • Sang Hyeon Jeong
  • Jong Hoon Lee
  • Ji Young Jung
  • Se Hee Lee
  • Moon Su Park
  • Che Ok Jeon
Original Paper

Abstract

Two moderately halophilic, facultatively aerobic, motile bacteria with flagella, designated strains 10-C-3T and 30-C-3, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of the strains were observed to be ovoid-rods showing catalase- and oxidase-positive reactions and production of creamy-pink pigments. Growth of strain 10-C-3T was observed at 15–35 °C (optimum, 25–30 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5), and in the presence of 3–15 % (w/v) salts (optimum: 5–10 %). The two strains were found to contain C18:1ω7c, C16:0, summed feature 3 (as defined by the MIDI system, comprising C16:1ω7c and/or C16:1ω6c), and C12:0 3-OH as the major cellular fatty acids. The G+C contents of the genomic DNA of strains 10-C-3T and 30-C-3 were determined to be 63.2 and 63.1 mol%, respectively and the respiratory quinone detected was ubiquinone 9 (Q-9) only. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 10-C-3Tand 30-C-3 formed a distinct phyletic lineage within the genus Halomonas and are most closely related to Halomonas fontilapidosi 5CRT with 95.2 % of 16S rRNA sequence similarity. Strains 10-C-3Tand 30-C-3 shared 99.2 % of 16S rRNA gene sequence similarity and their DNA–DNA relatedness value was 96.6 ± 0.9 %. On the basis of phenotypic, chemotaxonomic and molecular features, strains 10-C-3Tand 30-C-3 represent a novel species of the genus Halomonas, for which the name Halomonas cibimaris sp. nov. is proposed. The type strain is 10-C-3T (= KACC 14932T = JCM 16914T).

Keywords

Halomonas cibimaris Novel species Halophilic bacteria Fermented seafood Jeotgal 

Notes

Acknowledgments

These efforts were supported by the Technology Development Program for Agriculture and Forestry (TDPAF) of the Ministry for Agriculture, Forestry and Fisheries and the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Republic of Korea.

Supplementary material

10482_2012_9832_MOESM1_ESM.docx (658 kb)
Supplementary material 1 (DOCX 657 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Sang Hyeon Jeong
    • 1
  • Jong Hoon Lee
    • 2
  • Ji Young Jung
    • 1
  • Se Hee Lee
    • 1
  • Moon Su Park
    • 1
  • Che Ok Jeon
    • 1
  1. 1.Department of Life Science and Research Center for Biomolecules and BiosystemsChung-Ang UniversitySeoulRepublic of Korea
  2. 2.Department of Food Science and BiotechnologyKyonggi UniversitySuwonRepublic of Korea

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