Verrucosispora fiedleri sp. nov., an actinomycete isolated from a fjord sediment which synthesizes proximicins
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A novel filamentous actinobacterial organism, designated strain MG-37T, was isolated from a Norwegian fjord sediment and examined using a polyphasic taxonomic approach. The organism was determined to have chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora and formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree. It was most closely related to Verrucosispora maris DSM 45365T (99.5 % 16S rRNA gene similarity) and Verrucosispora gifhornensis DSM 44337T (99.4 % 16S rRNA gene similarity) but was distinguished from these strains based on low levels of DNA:DNA relatedness (~56 and ~50 %, respectively). It was readily delineated from all of the type strains of Verrucosispora species based on a combination of phenotypic properties. Isolate MG-37T (=NCIMB 14794T = NRRL-B-24892T) should therefore be classified as the type strain of a novel species of Verrucosispora for which the name Verrucosispora fiedleri is proposed.
KeywordsVerrucosispora fiedleri Polyphasic taxonomy Marine sediment Actinomycetes
ATB and MG were supported by the UK Natural Environmental Research Council (Grants NER/TS/S/2000/0614 NS NER/TS/S/2000/00616), JEMS by the Biotechnology and Biological Sciences Research Council (Grant CPA 1819) and Lixin Zhang by The Royal Society (Grant JP 090 697). WP is grateful to the DPST programme and the Royal Thai Government for financial support and ATB thanks the Leverhulme Trust for the award of an Emeritus Fellowship and JW for support by the Chinese Postdoctoral Science Foundation and the National Natural Science Foundation of China (Grant 31 000004). We are indebted to Dr Byung-Yong Kim for carrying out the fatty acid analysis and to Professor Gjert Knutsen (University of Bergen) and the crew of the F/F Hans Brattström for the collection of the fjord sample and to Professors Kui Hong (Wuhan University) and Ying Huang (Institute of Microbiology, Beijing) for the gifts of Verrucosispora type strains.
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