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Antonie van Leeuwenhoek

, Volume 103, Issue 3, pp 449–456 | Cite as

Geodermatophilus siccatus sp. nov., isolated from arid sand of the Saharan desert in Chad

  • Maria del Carmen Montero-Calasanz
  • Markus Göker
  • Manfred Rohde
  • Peter Schumann
  • Gabriele Pötter
  • Cathrin Spröer
  • Anna A. GorbushinaEmail author
  • Hans-Peter KlenkEmail author
Original Paper

Abstract

A novel Gram-positive, aerobic, actinobacterial strain, CF6/1T, was isolated in 2007 during environmental screening of arid desert soil in the Sahara near to Ourba, Chad. The isolate was found to grow best in a temperature range of 20–37 °C and at pH 6.0–8.5 and showed no NaCl tolerance, forming black-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics determined for the isolate match those previously described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was determined to be 74.9 mol %. The peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid. The main phospholipids were determined to be phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, diphosphatidylglycerol and traces of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose as the diagnostic sugar. The major cellular fatty acids were found to be the branched-chain saturated acids iso-C16:0 and iso-C15:0, as well as C17:1ω8c. The 16S rRNA gene sequence shows 97.5–97.9 % sequence identity with the four validly named or at least effectively published members of the genus: Geodermatophilus obscurus (97.5 %), Geodermatophilus arenarius (97.7 %), Geodermatophilus ruber (97.9 %) and Geodermatophilus nigrescens (97.9 %). Based on the results from this polyphasic taxonomic analysis and DNA–DNA hybridizations with all type strains of the genus, we propose that strain CF6/1T represents a novel species, Geodermatophilus siccatus, with the type strain CF6/1T = DSM 45419T = CCUG 62765T = MTCC 11414T.

Keywords

Actinomycetes Geodermatophilaceae Taxonomy Xerophiles Phenotype microarray 

Notes

Acknowledgments

We would like to gratefully acknowledge the help of Agathe Stricker of the International Committee of the Red Cross in Geneva, Switzerland, for organizing the collection of the sand samples from which strain CF6/1T was isolated. Jocelyne Favet, Arlette Cattaneo and William J. Broughton of the Laboratoire de Biologie Moléculaire de Plantes Supérieures (University of Geneva, Switzerland) are acknowledged for their contribution to the isolation of the strain, and Brian J. Tindall (DSMZ, Braunschweig) for his guidance in chemotaxonomic analyses. M.C. Montero-Calasanz is the recipient of a postdoctoral contract from European Social Fund Operational Programme (2007–2013) for Andalusia.

Supplementary material

10482_2012_9824_MOESM1_ESM.docx (383 kb)
Supplementary material 1 (DOCX 384 kb)
10482_2012_9824_MOESM2_ESM.tif (426 kb)
Supplementary material 2 (TIFF 427 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Maria del Carmen Montero-Calasanz
    • 1
    • 2
  • Markus Göker
    • 1
  • Manfred Rohde
    • 3
  • Peter Schumann
    • 1
  • Gabriele Pötter
    • 1
  • Cathrin Spröer
    • 1
  • Anna A. Gorbushina
    • 4
    • 5
    • 6
    Email author
  • Hans-Peter Klenk
    • 1
    Email author
  1. 1.DSMZ – German Collection of Microorganisms and Cell CulturesLeibniz InstituteBraunschweigGermany
  2. 2.IFAPA – Instituto de Investigación y Formación Agraria y Pesquera, Centro Las Torres-TomejilSevillaSpain
  3. 3.HZI – Helmholtz Centre for Infection ResearchBraunschweigGermany
  4. 4.BAM – Federal Institute for Material Research and TestingBerlinGermany
  5. 5.Department of Biology, Chemistry and PharmacyFree University of BerlinBerlinGermany
  6. 6.Department of Earth SciencesFree University of BerlinBerlinGermany

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