Geodermatophilus siccatus sp. nov., isolated from arid sand of the Saharan desert in Chad
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A novel Gram-positive, aerobic, actinobacterial strain, CF6/1T, was isolated in 2007 during environmental screening of arid desert soil in the Sahara near to Ourba, Chad. The isolate was found to grow best in a temperature range of 20–37 °C and at pH 6.0–8.5 and showed no NaCl tolerance, forming black-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics determined for the isolate match those previously described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was determined to be 74.9 mol %. The peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid. The main phospholipids were determined to be phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, diphosphatidylglycerol and traces of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose as the diagnostic sugar. The major cellular fatty acids were found to be the branched-chain saturated acids iso-C16:0 and iso-C15:0, as well as C17:1ω8c. The 16S rRNA gene sequence shows 97.5–97.9 % sequence identity with the four validly named or at least effectively published members of the genus: Geodermatophilus obscurus (97.5 %), Geodermatophilus arenarius (97.7 %), Geodermatophilus ruber (97.9 %) and Geodermatophilus nigrescens (97.9 %). Based on the results from this polyphasic taxonomic analysis and DNA–DNA hybridizations with all type strains of the genus, we propose that strain CF6/1T represents a novel species, Geodermatophilus siccatus, with the type strain CF6/1T = DSM 45419T = CCUG 62765T = MTCC 11414T.
KeywordsActinomycetes Geodermatophilaceae Taxonomy Xerophiles Phenotype microarray
We would like to gratefully acknowledge the help of Agathe Stricker of the International Committee of the Red Cross in Geneva, Switzerland, for organizing the collection of the sand samples from which strain CF6/1T was isolated. Jocelyne Favet, Arlette Cattaneo and William J. Broughton of the Laboratoire de Biologie Moléculaire de Plantes Supérieures (University of Geneva, Switzerland) are acknowledged for their contribution to the isolation of the strain, and Brian J. Tindall (DSMZ, Braunschweig) for his guidance in chemotaxonomic analyses. M.C. Montero-Calasanz is the recipient of a postdoctoral contract from European Social Fund Operational Programme (2007–2013) for Andalusia.
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