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Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 433–439 | Cite as

Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China

  • Ke Xing
  • Wei Liu
  • Yue-Ji Zhang
  • Guang-Kai Bian
  • Wen-Di Zhang
  • Tomohiko Tamura
  • Jung-Sook Lee
  • Sheng Qin
  • Ji-Hong Jiang
Original Paper

Abstract

A novel actinomycete, designated strain KLBMP 1262T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262T is most closely related to Amycolatopsis sulphurea DSM 46092T (97.96 %), Amycolatopsis ultiminotia RP-AC36T (97.50 %) and Amycolatopsis jejuensis N7-3T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C16:0, iso-C16:0 and iso-C15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262T (=KCTC 19885 T = NBRC 108679T).

Keywords

Amycolatopsis jiangsuensis sp. nov. Coastal plant Endophytic Polyphasic taxonomy 

Notes

Acknowledgments

The authors are grateful to Prof. Iain C. Sutcliffe for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB180008, 11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Natural Science Foundation by Xuzhou City (No. XZZD1004).

Supplementary material

10482_2012_9823_MOESM1_ESM.doc (475 kb)
Supplementary material 1 (DOC 475 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Ke Xing
    • 1
  • Wei Liu
    • 2
  • Yue-Ji Zhang
    • 1
  • Guang-Kai Bian
    • 1
  • Wen-Di Zhang
    • 1
  • Tomohiko Tamura
    • 3
  • Jung-Sook Lee
    • 4
  • Sheng Qin
    • 1
  • Ji-Hong Jiang
    • 1
  1. 1.The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu ProvinceSchool of Life Science, Jiangsu Normal UniversityXuzhouPeople’s Republic of China
  2. 2.Department of PediatricsAnyang Children’s HospitalAnyangPeople’s Republic of China
  3. 3.NITE Biological Resource Center (NBRC)National Institute of Technology and EvaluationKisarazuJapan
  4. 4.Korean Collection for Type Cultures, Biological Resource CenterKorea Research Institute of Bioscience and BiotechnologyYuseong-guRepublic of Korea

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