Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 433–439 | Cite as

Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China

  • Ke Xing
  • Wei Liu
  • Yue-Ji Zhang
  • Guang-Kai Bian
  • Wen-Di Zhang
  • Tomohiko Tamura
  • Jung-Sook Lee
  • Sheng Qin
  • Ji-Hong Jiang
Original Paper


A novel actinomycete, designated strain KLBMP 1262T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262T is most closely related to Amycolatopsis sulphurea DSM 46092T (97.96 %), Amycolatopsis ultiminotia RP-AC36T (97.50 %) and Amycolatopsis jejuensis N7-3T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C16:0, iso-C16:0 and iso-C15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262T (=KCTC 19885 T = NBRC 108679T).


Amycolatopsis jiangsuensis sp. nov. Coastal plant Endophytic Polyphasic taxonomy 



The authors are grateful to Prof. Iain C. Sutcliffe for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB180008, 11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Natural Science Foundation by Xuzhou City (No. XZZD1004).

Supplementary material

10482_2012_9823_MOESM1_ESM.doc (475 kb)
Supplementary material 1 (DOC 475 kb)


  1. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230PubMedCrossRefGoogle Scholar
  2. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
  3. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  4. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789CrossRefGoogle Scholar
  5. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N (1974) Nocardia coeliaca, Nocardia autotrophica, and the Nocardin strains. Int J Syst Bacteriol 24:54–63CrossRefGoogle Scholar
  6. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133PubMedCrossRefGoogle Scholar
  7. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322CrossRefGoogle Scholar
  8. He L, Li W, Huang Y, Wang L, Liu ZH (2005) Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:939–944CrossRefGoogle Scholar
  9. Kelly KL (1964) Color-name charts illustrated with centroid colors. Inter-Society Color Council-National Bureau of Standards, ChicagoGoogle Scholar
  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721PubMedCrossRefGoogle Scholar
  11. Kluge AG, Farris FS (1969) Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32CrossRefGoogle Scholar
  12. Kurup PV, Schmitt JA (1973) Numerical taxonomy of Nocardia. Can J Microbiol 19:1035–1048PubMedCrossRefGoogle Scholar
  13. Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443CrossRefGoogle Scholar
  14. Lechevalier MP, De Bièvre C, Lechevalier HA (1977) Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260CrossRefGoogle Scholar
  15. Lechevalier MP, Prauser H, Labeda DP, Ruan JS (1986) Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov. Int J Syst Bacteriol 36:29–37CrossRefGoogle Scholar
  16. Lee SD (2009) Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis. Int J Syst Evol Microbiol 59:1401–1404PubMedCrossRefGoogle Scholar
  17. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  18. Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95CrossRefGoogle Scholar
  19. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233CrossRefGoogle Scholar
  20. Qin S, Li J, Chen HH, Zhao GZ, Zhu WY, Jiang CL, Xu LH, Li WJ (2009) Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 75:6176–6186PubMedCrossRefGoogle Scholar
  21. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  22. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340CrossRefGoogle Scholar
  23. Stackebrandt E, Rainey FA, Ward-Rainey NL (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491CrossRefGoogle Scholar
  24. Takahashi Y (2001) Family Pseudonocardiaceae. In: The Society for Actinomycetes Japan (ed) Identification manual of Actinomycetes, The Business Centre for Academic Societies, Tokyo, pp 227–239Google Scholar
  25. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCrossRefGoogle Scholar
  26. Tan GYA, Goodfellow M (2012) Genus IV. Amycolatopsis (Lechevalier, Prauser, Labeda and Ruan 1986) Lee 2009, 1403VI. In: Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME, Suzuki KI, Ludwig W, Whitman WB (eds) Bergey’s Manual of Systematic Bacteriology, 2nd edn, vol. 5. The Actinobacteria, Springer, Berlin, pp 1335–1336Google Scholar
  27. Tang SK, Wang Y, Guan TW, Lee JC, Kim CJ, Li WJ (2010) Amycolatopsis halophila sp. nov., a halophilic actinomycete isolated from a salt lake. Int J Syst Evol Microbiol 60:1073–1078PubMedCrossRefGoogle Scholar
  28. Waksman SA (1967) The Actinomycetes. A Summary of Current Knowledge. Ronald Press, New YorkGoogle Scholar
  29. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH et al (1987) International 21 committee on systematic bacteriology. Report of the ad hoc committee on reconciliation 22 of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  30. Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2452–2492Google Scholar
  31. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153PubMedCrossRefGoogle Scholar
  32. Zucchi TD, Tan GYA, Goodfellow M (2012) Amycolatopsis thermophila sp. nov. and Amycolatopsis viridis sp. nov., novel thermophilic actinomycetes isolated from Australian soil. Int J Syst Evol Microbiol 62:168–172PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Ke Xing
    • 1
  • Wei Liu
    • 2
  • Yue-Ji Zhang
    • 1
  • Guang-Kai Bian
    • 1
  • Wen-Di Zhang
    • 1
  • Tomohiko Tamura
    • 3
  • Jung-Sook Lee
    • 4
  • Sheng Qin
    • 1
  • Ji-Hong Jiang
    • 1
  1. 1.The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu ProvinceSchool of Life Science, Jiangsu Normal UniversityXuzhouPeople’s Republic of China
  2. 2.Department of PediatricsAnyang Children’s HospitalAnyangPeople’s Republic of China
  3. 3.NITE Biological Resource Center (NBRC)National Institute of Technology and EvaluationKisarazuJapan
  4. 4.Korean Collection for Type Cultures, Biological Resource CenterKorea Research Institute of Bioscience and BiotechnologyYuseong-guRepublic of Korea

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