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Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 421–431 | Cite as

Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel d-xylose-fermenting yeast species from Brazilian Amazonian forest

  • Raquel M. Cadete
  • Monaliza A. Melo
  • Jerri E. Zilli
  • Marcos J. S. Vital
  • Adriane Mouro
  • Alice H. Prompt
  • Fátima C. O. Gomes
  • Boris U. Stambuk
  • Marc-André Lachance
  • Carlos A. Rosa
Original Paper

Abstract

Four new d-xylose fermenting yeast species of the clade Spathaspora were recovered from rotting-wood samples in a region of Amazonian forest, Northern Brazil. Three species produced unconjugated asci with a single elongated ascospore with curved ends. These species are described as Spathaspora brasiliensis, Spathaspora suhii and Spathaspora roraimanensis. Two isolates of an asexually reproducing species belonging to the Spathaspora clade were also obtained and they are described as Spathaspora xylofermentans. All these species are able to ferment d-xylose during aerobic batch growth in rich YP (1 % yeast extract, 2 % peptone and 2 % D-xylose) medium, albeit with differing efficiencies. The type strains are Spathaspora brasiliensis sp. nov UFMG-HMD19.3 (=CBMAI 1425=CBS 12679), Spathaspora suhii sp. nov. UFMG-XMD16.2 (=CBMAI 1426=CBS 12680), Spathaspora roraimanensis sp. nov. UFMG-XMD23.2 (CBMAI 1427=CBS 12681) and Spathaspora xylofermentans sp. nov. UFMG-HMD23.3 (=CBMAI 1428=CBS 12682).

Keywords

New yeast species Spathaspora d-xylose-fermenting yeast Rotting wood Amazonian forest 

Notes

Acknowledgments

This work was supported in part by grants and fellowships from the Brazilian agencies Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, process no. 552877/2007-7, 551392/2010-0, 551245/2010-7, 560715/2010-2, and 490029/2009-4), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Procad-NF, process no. 2280/2008), Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP/BIOEN/FAPEMIG, process no. 2008/57926-4), Fundação de Apoio à Pesquisa Científica e Tecnológica do Estado de Santa Catarina (FAPESC, process no. 17293/2009-6), and the Natural Science and Engineering Research Council of Canada (M.-A. L.).

Conflict of interest

None.

References

  1. Alves SL Jr, Herberts RA, Hollatz C, Miletti LC, Stambuk BU (2007) Maltose and maltotriose active transport and fermentation by Saccharomyces cerevisiae. J Am Soc Brew Chem 65:99–104Google Scholar
  2. Amorim HV, Lopes ML, de Castro Oliveira JV, Buckeridge MS, Goldman GH (2011) Scientific challenges of bioethanol production in Brazil. Appl Microbiol Biotechnol 91:1267–1275PubMedCrossRefGoogle Scholar
  3. Barbosa AC, Cadete RM, Gomes FCO, Lachance MA, Rosa CA (2009) Candida materiae sp. nov., a yeast species isolated from rotting wood in the Atlantic Rain Forest. Int J Syst Evol Microbiol 59:2104–2106PubMedCrossRefGoogle Scholar
  4. Boonmak C, Limtong S, Jindamorakot S, Am-In S, Yongmanitchai W, Suzuki K, Nakase T, Kawasaki H (2011) Candida xylanilytica sp. nov., a xylan-degrading yeast species isolated from Thailand. Int J Syst Evol Microbiol 61:1230–1234PubMedCrossRefGoogle Scholar
  5. Cadete RM, Santos RO, Melo MA, Mouro A, Gonçalves DL, Stambuk BU, Gomes FCO, Lachance MA, Rosa CA (2009) Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 9:1338–1342PubMedCrossRefGoogle Scholar
  6. Cheng JJ, Timilsina GR (2011) Status and barriers of advanced biofuel technologies: a review. Renew Energ 36:3541–3549CrossRefGoogle Scholar
  7. da Cunha-Pereira F, Hickert LR, Sehnem NT, de Souza-Cruz PB, Rosa CA, Ayub MAS (2011) Conversion of sugars present in rice hull hydrolysates into ethanol by Spathaspora arborariae, Saccharomyces cerevisiae, and their co-fermentations. Biores Technol 102:4218–4225CrossRefGoogle Scholar
  8. Hahn-Hägerdal B, Galbe M, Gorwa-Grauslund MF, Lidén G, Zacchi G (2006) Bio-ethanol—the fuel of tomorrow from the residues of today. Trends Biotechnol 24:549–556PubMedCrossRefGoogle Scholar
  9. Hahn-Hägerdal B, Karhumaa K, Fonseca C, Spencer-Martins I, Gorwa-Grauslund MF (2007) Towards industrial pentose-fermenting yeast strains. Appl Microbiol Biotechnol 74:937–953PubMedCrossRefGoogle Scholar
  10. Hou X (2012) Anaerobic xylose fermentation by Spathaspora passalidarum. Appl Microbiol Biotechnol 94:205–214PubMedCrossRefGoogle Scholar
  11. Kurtzman CP, Fell JW, Boekhout T, Robert V (2011a) Methods for isolation phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts: a taxonomic study, 5th edn. Elsevier, New York, pp 87–110CrossRefGoogle Scholar
  12. Kurtzman CP, Fell JW, Boekhout T (2011b) The yeasts: a taxonomic study, 5th edn. Elsevier, AmsterdamGoogle Scholar
  13. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP (2011) Candida Berkhout, (1923). In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts: a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 987–1278CrossRefGoogle Scholar
  14. Long TM, Su YK, Headman J, Higbee A, Willis LB, Jeffries TW (2012) Cofermentation of glucose, xylose, and cellobiose by the beetle-associated yeast, Spathaspora passalidarum. Appl Environ Microbiol 78:5492–5500PubMedCrossRefGoogle Scholar
  15. Marinoni G, Lachance MA (2004) Speciation in the large-spored Metschnikowia clade and establishment of a new species, Metschnikowia borealis comb. nov. FEMS Yeast Res 4:587–596PubMedCrossRefGoogle Scholar
  16. Merico A, Sulo P, Piskur J, Compagno C (2007) Fermentative lifestyle in yeasts belonging to the Saccharomyces complex. FEBS J 274:976–989PubMedCrossRefGoogle Scholar
  17. Miller GL (1959) Use of dinitrosalycilic acid reagent for determination of reducing sugar. Anal Chem 31:426–429CrossRefGoogle Scholar
  18. Nguyen NH, Suh SO, Marshall CJ, Blackwell M (2006) Morphological and ecological similarities: wood-boring beetles associated with novel xylose-fermenting yeasts, Spathaspora passalidarum gen. sp. nov. and Candida jeffriesii sp. nov. Mycol Res 110:1232–1241PubMedCrossRefGoogle Scholar
  19. Nguyen NH, Suh S-O, Blackwell M (2011) Spathaspora Nguyen, Suh and Blackwell. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts: a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 795–797CrossRefGoogle Scholar
  20. Norvell LL (2011) Fungal nomenclature. 1.Melbourne approves a new code. Mycotaxon 116:481–490CrossRefGoogle Scholar
  21. Rozpędowska E, Galafassi S, Johansson L, Hagman A, Piškur J, Compagno C (2011) Candida albicans—a pre-whole genome duplication yeast—is predominantly aerobic and a poor ethanol producer. FEMS Yeast Res 11:285–291PubMedCrossRefGoogle Scholar
  22. Solomon BD (2010) Biofuels and sustainability. Ann NY Acad Sci 1185:119–134PubMedCrossRefGoogle Scholar
  23. Stambuk BU, Franden MA, Singh A, Zhang M (2003) d-Xylose transport by Candida succiphila and Kluyveromyces marxianus. Appl Biochem Biotechnol 105:255–263PubMedCrossRefGoogle Scholar
  24. Stambuk BU, Eleutherio ECA, Florez-Pardo LM, Souto-Maior AM, Bon EPS (2008) Brazilian potential for biomass ethanol: challenge of using hexose and pentose co-fermenting yeast strains. J Sci Ind Res 67:918–926Google Scholar
  25. Suh S-O, Marshall CJ, McHugh JV, Blackwell M (2003) Wood ingestion by passalid beetles in the presence of xylose-fermenting gut yeasts. Mol Ecol 12:3137–3145PubMedCrossRefGoogle Scholar
  26. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCrossRefGoogle Scholar
  27. Wohlbach DJ, Kuo A, Sato TK, Potts KM, Salamov AA, Labutti KM, Sun H, Clum A, Pangilinan JL, Lindquist EA, Lucas S, Lapidus A, Jin M, Gunawan C, Balan V, Dale BE, Jeffries TW, Zinkel R, Barry KW, Grigoriev IV, Gasch AP (2011) Comparative genomics of xylose-fermenting fungi for enhanced biofuel production. Proc Natl Acad Sci USA 108:13212–13217PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Raquel M. Cadete
    • 1
  • Monaliza A. Melo
    • 1
  • Jerri E. Zilli
    • 2
  • Marcos J. S. Vital
    • 3
  • Adriane Mouro
    • 4
  • Alice H. Prompt
    • 4
  • Fátima C. O. Gomes
    • 5
  • Boris U. Stambuk
    • 4
  • Marc-André Lachance
    • 6
  • Carlos A. Rosa
    • 1
  1. 1.Departamento de MicrobiologiaInstituto de Ciências Biológicas (ICB), C.P. 486, Universidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Embrapa AgrobiologiaSeropédicaBrazil
  3. 3.Departamento de BiologiaUniversidade Federal de Roraima - Campus do ParicaranaBoa VistaBrazil
  4. 4.Departamento de Bioquímica, Centro de Ciências BiológicasUniversidade Federal de Santa CatarinaFlorianópolisBrazil
  5. 5.Departamento de QuímicaCentro Federal de Educação Tecnológica de Minas GeraisBelo HorizonteBrazil
  6. 6.Department of BiologyUniversity of Western OntarioLondonCanada

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