Barcoded pyrosequencing analysis of the microbial community in a simulator of the human gastrointestinal tract showed a colon region-specific microbiota modulation for two plant-derived polysaccharide blends
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The combination of a Simulator of the Human Intestinal Microbial Ecosystem with ad hoc molecular techniques (i.e. pyrosequencing, denaturing gradient gel electrophoresis and quantitative PCR) allowed an evaluation of the extent to which two plant polysaccharide supplements could modify a complex gut microbial community. The presence of Aloe vera gel powder and algae extract in product B as compared to the standard blend (product A) improved its fermentation along the entire simulated colon. The potential extended effect of product B in the simulated distal colon, as compared to product A, was confirmed by: (i) the separate clustering of the samples before and after the treatment in the phylogenetic-based dendrogram and OTU-based PCoA plot only for product B; (ii) a higher richness estimator (+33 vs. −36 % of product A); and (iii) a higher dynamic parameter (21 vs. 13 %). These data show that the combination of well designed in vitro simulators with barcoded pyrosequencing is a powerful tool for characterizing changes occurring in the gut microbiota following a treatment. However, for the quantification of low-abundance species—of interest because of their relationship to potential positive health effects (i.e. bifidobacteria or lactobacilli)—conventional molecular ecological approaches, such as PCR–DGGE and qPCR, still remain a very useful complementary tool.
KeywordsGastrointestinal resource management (GRM) Pyrosequencing Ambrotose SHIME Intestinal bacteria
Tom Van de Wiele, Sam Possemiers and Massimo Marzorati are Postdoctoral Fellows of the Research Foundation—Flanders (FWO, Belgium).
- De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S, Collini S, Pieraccini G, Lionetti P (2010) Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proc Natl Acad Sci USA 107:14691–14696PubMedCrossRefGoogle Scholar
- Grootaert C, Van den Abbeele P, Marzorati M, Broekaert WF, Courtin CM, Delcour JA, Verstraete W, Van de Wiele T (2009) Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS Microbiol Ecol 69:231–242PubMedCrossRefGoogle Scholar
- Lorenz MO (1905) Methods of measuring concentration of wealth. J Am Stat Assoc 9:209–219Google Scholar
- Marzorati M, Possemiers S, Verstraete W (2009) The use of the SHIME-related technology platform to assess the efficacy of pre- and probiotics. Agro Food Ind Hi-Tech 20:S50–S53Google Scholar
- Ramberg JE, Nelson ED, Jacoby HI, Li Q, Koetzner L, Zhang B, Boulet J, Sinnot RA (2010) Plant plysaccharide supplements reduce the expression of pro-inflammatory genes in colonic tissue of mice with dextran sulfate sodium-induced colitis. Int J Probiotics Prebiotics 5:229–236Google Scholar
- Sanchez JI, Marzorati M, Grootaert C, Baran M, Van Craeyveld V et al (2009) Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the simulator of human intestinal microbial ecosystem. Microb Biotechnol 2:101–113PubMedCrossRefGoogle Scholar