Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 399–408

Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae

  • Feiyu Jia
  • Chongxi Liu
  • Xiangjing Wang
  • Junwei Zhao
  • Qianfeng Liu
  • Ji Zhang
  • Ruixia Gao
  • Wensheng Xiang
Original Paper

Abstract

A novel endophytic actinomycete, designated strain NEAU-J3T, was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain NEAU-J3T fell within the family Micromonosporaceae. The strain was observed to form an extensively branched substrate mycelium, which carried non-motile oval spores with a smooth surface. The cell walls of strain NEAU-J3T were determined to contain meso-diaminopimelic acid and galactose, ribose and glucose were detected as whole-cell sugars. The major menaquinones were determined to be MK-9(H4) and MK-9(H6). The phospholipids detected were phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were determined to be C16:0, C18:1 ω9c, C18:0, C17:0, C17:1 ω7c, anteiso-C17:0, C16:1 ω7c and C15:0. The DNA G + C content was 62.5 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J3T is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Wangella harbinensis gen. nov., sp. nov. is proposed. The type strain of Wangella harbinensis is strain NEAU-J3T (=CGMCC 4.7039T = DSM 45747T).

Keywords

Wangella harbinensis gen. nov. Sp. nov. Polyphasic taxonomy 16S rRNA gene 

Supplementary material

10482_2012_9820_MOESM1_ESM.doc (58 kb)
Supplementary material 1 (DOC 58 kb)
10482_2012_9820_MOESM2_ESM.doc (59 kb)
Supplementary material 2 (DOC 59 kb)
10482_2012_9820_MOESM3_ESM.doc (73 kb)
Supplementary material 3 (DOC 73 kb)
10482_2012_9820_MOESM4_ESM.doc (136 kb)
Supplementary material 4 (DOC 135 kb)
10482_2012_9820_MOESM5_ESM.doc (94 kb)
Supplementary material 5 (DOC 94 kb)
10482_2012_9820_MOESM6_ESM.doc (36 kb)
Supplementary material 6 (DOC 36 kb)
10482_2012_9820_MOESM7_ESM.doc (36 kb)
Supplementary material 7 (DOC 36 kb)

References

  1. Ara I, Kudo T (2006) Three novel species of the genus Catellatospora, Catellatospora chokoriensis sp. nov., Catellatospora coxensis sp. nov. and Catellatospora bangladeshensis sp. nov., and transfer of Catellatospora citrea subsp. methionotrophica Asano and Kawamoto 1988 to Catellatospora methionotrophica sp. nov., comb. nov. Int J Syst Evol Microbiol 56:393–400PubMedCrossRefGoogle Scholar
  2. Ara I, Kudo T (2007a) Luedemannella gen. nov., a new member of the family Micromonosporaceae and description of Luedemannella helvata sp. nov. and Luedemannella flava sp. nov. J Gen Appl Microbiol 53:39–51PubMedCrossRefGoogle Scholar
  3. Ara I, Kudo T (2007b) Krasilnikovia gen. nov., a new member of the family Micromonosporaceae and description of Krasilnikovia cinnamomea sp. nov. Actinomycetologica 21:1–10CrossRefGoogle Scholar
  4. Ara I, Bakir MA, Kudo T (2008a) Transfer of Catellatospora koreensis Lee et al. 2000 as Catelliglobosispora koreensis gen. nov., comb. nov. and Catellatospora tsunoense Asano et al. 1989 as Hamadaea tsunoensis gen. nov., comb. nov., and emended description of the genus Catellatospora Asano and Kawamoto 1986 emend. Lee and Hah 2002. Int J Syst Evol Microbiol 58:1950–1960PubMedCrossRefGoogle Scholar
  5. Ara I, Matsumoto A, Bakir MA, Kudo T, Omura S, Takahashi Y (2008b) Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 58:1644–1652PubMedCrossRefGoogle Scholar
  6. Asano K, Kawamoto I (1986) Catellatospora, a new genus of the Actinomycetales. Int J Syst Bacteriol 36:512–517CrossRefGoogle Scholar
  7. Brosius J, Palmer JL, Kennedy JP, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75:4801–4805PubMedCrossRefGoogle Scholar
  8. Collins MD (1985) Isoprenoid quinone analyses in bacterial classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic, London, pp 267–284Google Scholar
  9. Couch JN (1950) Actinoplanes, a new genus of the Actinomycetales. J Elisha Mitchell Sci Soc 66:87–92Google Scholar
  10. Cross T, Macivera AM, Lacey J (1968) The thermophilic actinomycetes in mouldy hay: micro polyspora faeni sp. nov. J Gen Microbiol 50:351–359PubMedCrossRefGoogle Scholar
  11. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376PubMedCrossRefGoogle Scholar
  12. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  13. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  14. Goodfellow ML, Stanton J, Simpson KE, Minnikin DE (1990) Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136:19–36CrossRefGoogle Scholar
  15. Gordon RE, Barnett DA, Handerhan JE, Pang C (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63CrossRefGoogle Scholar
  16. Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509CrossRefGoogle Scholar
  17. Inahashi Y, Matsumoto A, Danbara H, Omura S, Takahashi Y (2010) Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from a plant root. Int J Syst Evol Microbiol 60:2652–2658PubMedCrossRefGoogle Scholar
  18. Jacobson E, Grauville WC, Fogs CE (1958) Color harmony manual, 4th edn. Container Corporation of America, ChicagoGoogle Scholar
  19. Jones KL (1949) Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145PubMedGoogle Scholar
  20. Kane WD (1966) A new genus of the Actinoplanaceae, Pilimelia, with a description of two species, Pilimelia terevasa and Pilimelia anulata. J Elisha Mitchell Sci Soc 82:220–230Google Scholar
  21. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  22. Koch C, Kroppenstedt RM, Rainey FA, Stackebrandt E (1996) 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae. Int J Syst Bacteriol 46:765–768PubMedCrossRefGoogle Scholar
  23. Krasil’nikov NA (1938) Ray fungi and related organisms Actinomycetales. Izdatel’stvo Akademii Nauk SSSR, MoscowGoogle Scholar
  24. Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics (Society for Applied Bacteriology Technical Series), vol 20. Academic, New York, pp 173–199Google Scholar
  25. Kudo T, Nakajima Y, Suzuki K (1999) Catenuloplanes crispus (Petrolini et al. 1993) comb. nov.: incorporation of the genus Planopolyspora Petrolini 1993 into the genus Catenuloplanes Yokota et al. 1993 with an amended description of the genus Catenuloplanes. Int J Syst Bacteriol 49:1853–1860PubMedCrossRefGoogle Scholar
  26. Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Actinomycete Taxonomy (Special Publication no. 6), Arlington: Society for Industrial Microbiology, pp. 277–284 Google Scholar
  27. Lechevalier MP, Stern AE, Lechevalier HA (1981) Phospholipids in the taxonomy of actinomycetes. In: Schaal KP, Pulverer G (eds) Actinomycete taxonomy. Gustav Fischer, New York, pp 111–116Google Scholar
  28. Lee SD, Hah YC (2002) Proposal to transfer Catellatospora ferruginea and ‘Catellatospora ishikariense’ to Asanoa gen. nov. as Asanoa ferruginea comb. nov. and Asanoa ishikariensis sp. nov., with emended description of the genus Catellatospora. Int J Syst Evol Microbiol 52:967–972PubMedCrossRefGoogle Scholar
  29. Lee DW, Lee SD (2011) Allocatelliglobosispora scoriae gen. nov., sp. nov., isolated from volcanic ash. Int J Syst Evol Microbiol 61:264–270PubMedCrossRefGoogle Scholar
  30. Lee YK, Kim HW, Liu CL, Lee HK (2003) A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52:245–250PubMedCrossRefGoogle Scholar
  31. Li J, Zhao GZ, Zhu WY, Huang HY, Xu LH, Zhang S, Li WJ (2011) Phytomonospora endophytica gen. nov., sp. nov., isolated from the roots of Artemisia annua L. Int J Syst Evol Microbiol 61:2967–2973PubMedCrossRefGoogle Scholar
  32. Loqman S, Barka EA, Clément C, Ouhdouch Y (2009) Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. World J Microbiol Biotechnol 25:81–91CrossRefGoogle Scholar
  33. Maldonado LA, Fenical W, Jensen PR, Kauffman CA, Mincer TJ, Ward AC, Bull AT, Goodfellow M (2005) Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae. Int J Syst Evol Microbiol 55:1759–1766PubMedCrossRefGoogle Scholar
  34. Mandel M, Marmur J (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206CrossRefGoogle Scholar
  35. Matsumoto A, Takahashi Y, Shinose M, Seino A, Iwai Y, Omura S (2003) Longispora albida gen. nov., sp. nov., a novel genus of the family Micromonosporaceae. Int J Syst Evol Microbiol 53:1553–1559PubMedCrossRefGoogle Scholar
  36. McKerrow J, Vagg S, McKinney T, Seviour EM, Maszenan AM, Brooks P, Seviour RJ (2000) A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30:178–182PubMedCrossRefGoogle Scholar
  37. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JK (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  38. Monciardini P, Cavaletti L, Ranghetti A, Schumann P, Rohde M, Bamonte R, Sosio M, Mezzelani A, Donadio S (2009) Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov. Int J Syst Evol Microbiol 59:2752–2758PubMedCrossRefGoogle Scholar
  39. Ørskov J (1923) Investigations into the morphology of the ray fungi. Levin and Munksgaard, CopenhagenGoogle Scholar
  40. Qin S, Li J, Zhang YQ, Zhu WY, Zhao GZ, Xu LH, Li WJ (2009) Plantactinospora mayteni gen. nov., sp. nov., a member of the family Micromonosporaceae. Int J Syst Evol Microbiol 59:2527–2533PubMedCrossRefGoogle Scholar
  41. Rheims H, Schumann P, Rohde M, Stackebrandt E (1998) Verrucosispora gifhornensis gen. nov., sp. nov., a new member of the actinobacterial family Micromonosporaceae. Int J Syst Bacteriol 48:1119–1127PubMedCrossRefGoogle Scholar
  42. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  43. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340CrossRefGoogle Scholar
  44. Stackebrandt E, Rainey FA, Ward-Rainey NL (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491CrossRefGoogle Scholar
  45. Tamura T, Nakagaito Y, Nishii T, Hasegawa T, Stackebrandt E, Yokota A (1994) A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44:193–203PubMedCrossRefGoogle Scholar
  46. Tamura T, Hayakawa M, Hatano K (1997) A new genus of the order Actinomycetales, Spirilliplanes gen. nov., with description of Spirilliplanes yamanashiensis sp. nov. Int J Syst Bacteriol 47:97–102PubMedCrossRefGoogle Scholar
  47. Tamura T, Hayakawa M, Hatano K (2001) A new genus of the order Actinomycetales, Virgosporangium gen. nov., with descriptions of Virgosporangium ochraceum sp. nov. and Virgosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51:1809–1816PubMedCrossRefGoogle Scholar
  48. Tamura T, Hatano K, Suzuki K (2006) A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov. Int J Syst Evol Microbiol 56:1959–1964PubMedCrossRefGoogle Scholar
  49. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCrossRefGoogle Scholar
  50. Thawai C, Tanasupawat S, Itoh T, Kudo T (2006) Actinocatenispora thailandica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 56:1789–1794PubMedCrossRefGoogle Scholar
  51. Thawai C, Tanasupawat S, Suwanborirux K, Kudo T (2010) Actinaurispora siamensis gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 60:1660–1666PubMedCrossRefGoogle Scholar
  52. Thiemann JE, Pagani H, Beretta G (1967) A new genus of the Actinoplanaceae: Dactylosporangium, gen. nov. Arch Mikrobiol 58:42–52PubMedCrossRefGoogle Scholar
  53. Uchida K, Kudo T, Suzuki K, Nakase T (1999) A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56PubMedCrossRefGoogle Scholar
  54. Wiese J, Jiang Y, Tang SK, Thiel V, Schmaljohann R, Xu LH, Jiang CL, Imhoff JF (2008) A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov. Int J Syst Evol Microbiol 58:1324–1331PubMedCrossRefGoogle Scholar
  55. Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin MJ (1983) Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813PubMedGoogle Scholar
  56. Wu C, Lu X, Qin M, Wang Y, Ruan J (1989) Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 16:176–178Google Scholar
  57. Xiang WS, Liu CX, Wang XJ, Du J, Xi LJ, Huang Y (2011) Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 61:1165–1169PubMedCrossRefGoogle Scholar
  58. Xie QY, Wang C, Wang R, Qu Z, Lin HP, Hong K (2011) Jishengella endophytica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 61:1153–1159PubMedCrossRefGoogle Scholar
  59. Yokota A, Tamura T, Hasegawa T, Huang LH (1993) Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales. Int J Syst Bacteriol 43:805–812CrossRefGoogle Scholar
  60. Zhi XY, Li WJ, Stackebrandt E (2009) An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Feiyu Jia
    • 1
  • Chongxi Liu
    • 1
  • Xiangjing Wang
    • 1
  • Junwei Zhao
    • 1
  • Qianfeng Liu
    • 1
  • Ji Zhang
    • 1
  • Ruixia Gao
    • 1
  • Wensheng Xiang
    • 1
  1. 1.School of Life ScienceNortheast Agricultural UniversityHarbinPeople’s Republic of China

Personalised recommendations