Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 313–319 | Cite as

Trichosporon vanderwaltii sp. nov., an asexual basidiomycetous yeast isolated from soil and beetles

  • Thabiso E. Motaung
  • Jacobus Albertyn
  • Johan L. F. Kock
  • Ching-Fu Lee
  • Sung-Oui Suh
  • Meredith Blackwell
  • Carolina H. Pohl
Original Paper

Abstract

During a survey of unidentified yeast isolates deposited in the UNESCO-MIRCEN Biotechnological Yeast Culture Collection housed at the Department of Microbial, Biochemical and Food Biotechnology of the University of the Free State, one isolate obtained from soil in South Africa showed 100 % identity in D1/D2 rDNA sequence with undescribed basidiomycetous yeasts isolated from the gut of beetles from the United States of America and forest soil from Taiwan in the NCBI sequence database. Phylogenetic analyses using sequences of the D1/D2 rDNA and ITS regions indicated that all these isolates form a well-supported sub-clade that is the sister clade to the Brassicae plus Porosum clades of Trichosporon in the order Trichosporonales. Subsequent phenotypic tests revealed that asexual reproduction by budding is rare but dominated by arthroconidia resulting from segmentation of hyphae and that fusiform giant cells are characterized by budding from a broad base. These findings further suggest that these isolates belong to a single tremellomycetous yeast species for which the name Trichosporon vanderwaltii CBS 12124T (=NRRL Y-48732T, =UOFS Y-1920T) is proposed.

Keywords

Trichosporon T. vanderwaltii sp. nov. D1/D2 rDNA sequence ITS sequence Taxonomy 

Notes

Acknowledgments

This work of TEM, JA, JLFK and CHP was supported by the National Research Foundation and Department of Science and Technology under the SABI Programme (Grant Number 75048). Any opinion, findings and conclusions or recommendations expressed in this material are those of the authors and therefore the NRF and DST do not accept any liability in regard thereto. The contributions of MB and S-OS was supported by the US National Science Foundation (DEB-0072741and DEB-0417180) and the Boyd Professor Research Fund. We thank Hector Urbina, who revived the strains from beetles and Andri Van Wyk, who revived the South African strain.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Thabiso E. Motaung
    • 1
  • Jacobus Albertyn
    • 1
  • Johan L. F. Kock
    • 1
  • Ching-Fu Lee
    • 2
  • Sung-Oui Suh
    • 3
  • Meredith Blackwell
    • 4
  • Carolina H. Pohl
    • 1
  1. 1.Department of Microbial, Biochemical, and Food BiotechnologyUniversity of the Free StateBloemfonteinSouth Africa
  2. 2.Department of Applied ScienceNational Hsinchu University of EducationHsinchuTaiwan
  3. 3.American Type Culture Collection (ATCC)ManassasUSA
  4. 4.Department of Biological SciencesLouisiana State UniversityBaton RougeUSA

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