Antonie van Leeuwenhoek

, Volume 103, Issue 2, pp 293–298 | Cite as

Actinoplanes hulinensis sp. nov., a novel actinomycete isolated from soybean root (Glycine max (L.) Merr)

  • Yue Shen
  • Chongxi Liu
  • Xiangjing Wang
  • Junwei Zhao
  • Feiyu Jia
  • Yuqin Zhang
  • Liang Wang
  • Deguang Yang
  • Wensheng Xiang
Original Paper


A novel actinomycete, designated strain NEAU-M9T, was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-M9T belonged to the genus Actinoplanes, being most closely related to Actinoplanes campanulatus DSM 43148T (98.85 %), Actinoplanes capillaceus DSM 44859T (98.70 %), Actinoplanes lobatus DSM 43150T (98.30 %), Actinoplanes auranticolor DSM 43031T (98.23 %) and Actinoplanes sichuanensis 03-723T (98.06 %); similarity to other type strains of the genus Actinoplanes ranged from 95.87 to 97.56 %. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with A. campanulatus DSM 43148T and A. capillaceus DSM 44859T. This branching pattern was also supported by the tree constructed with the maximum-likelihood method. However, the low level of DNA–DNA relatedness allowed the isolate to be differentiated from the above-mentioned two Actinoplanes species. Moreover, strain NEAU-M9T could also be distinguished from the most closely related species by morphological, physiological and characteristics. Therefore, it is proposed that strain NEAU-M9T represents a novel Actinoplanes species, Actinoplanes hulinensis sp. nov. The type strain of Actinoplanes hulinensis is NEAU-M9T (= CGMCC 4.7036T = DSM 45728T).


Actinoplanes hulinensis sp. nov. Polyphasic taxonomy 16S rRNA gene 



This work was supported in part by grants from NKPBR (No. 2010CB126102) and NNSF (No. 30971937 and 30771427) of China, the Special Fundation for Scientific and Technological Innovation Research of Harbin (No. 2011RFXXN038), NSF of Heilongjiang Province (No. C201029), and NIH grant GM086184.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Yue Shen
    • 1
  • Chongxi Liu
    • 2
  • Xiangjing Wang
    • 2
  • Junwei Zhao
    • 2
  • Feiyu Jia
    • 2
  • Yuqin Zhang
    • 3
  • Liang Wang
    • 2
  • Deguang Yang
    • 1
  • Wensheng Xiang
    • 2
  1. 1.College of AgricultureNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.School of Life ScienceNortheast Agricultural UniversityHarbinPeople’s Republic of China
  3. 3.Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China

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