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Antonie van Leeuwenhoek

, Volume 103, Issue 1, pp 121–133 | Cite as

Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum

  • Erick Francisco RakotonirianaEmail author
  • Mamy Rafamantanana
  • Denis Randriamampionona
  • Christian Rabemanantsoa
  • Suzanne Urveg-Ratsimamanga
  • Mondher El Jaziri
  • Françoise Munaut
  • Anne-Marie Corbisier
  • Joëlle Quetin-Leclercq
  • Stéphane Declerck
Original Paper

Abstract

Thirty-one endophytic bacteria isolated from healthy leaves of Centella asiatica were screened in vitro for their ability to reduce the growth rate and disease incidence of Colletotrichum higginsianum, a causal agent of anthracnose. Isolates of Cohnella sp., Paenibacillus sp. and Pantoea sp. significantly stimulated the growth rate of C. higginsianum MUCL 44942, while isolates of Achromobacter sp., Acinetobacter sp., Microbacterium sp., Klebsiella sp. and Pseudomonas putida had no influence on this plant pathogen. By contrast, Bacillus subtilis BCA31 and Pseudomonas fluorescens BCA08 caused a marked inhibition of C. higginsianum MUCL 44942 growth by 46 and 82 %, respectively. Cell-free culture filtrates of B. subtilis BCA31 and P. fluorescens BCA08 were found to contain antifungal compounds against C. higginsianum MUCL 44942. Inoculation assays on in vitro-cultured plants of C. asiatica showed that foliar application of B. subtilis BCA31, three days before inoculation with C. higginsianum MUCL 44942, significantly reduced incidence and severity of the disease. The role of endophytic bacteria in maintaining the apparent inactivity of C. higginsianum MUCL 44942 in C. asiatica grown in the wild is discussed.

Keywords

Antifungal activity Centella asiatica Colletotrichum higginsianum Endophytic bacteria In vitro plants 

Notes

Acknowledgments

Erick Francisco Rakotoniriana is grateful to ADRI/UCL (Administration de la Relation Internationale/Université catholique de Louvain, Belgium) for the doctoral fellowship allowing him to perform this research. We also thank the Laboratory of Food and Environmental Microbiology (UCL, Belgium) for providing technical assistance and facilities in bacterial identification. We wish to thank the Belgian Commission Universitaire pour le Développement (CUD) for contributing financially to this research.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Erick Francisco Rakotoniriana
    • 1
    • 3
    Email author
  • Mamy Rafamantanana
    • 3
  • Denis Randriamampionona
    • 3
  • Christian Rabemanantsoa
    • 3
  • Suzanne Urveg-Ratsimamanga
    • 3
  • Mondher El Jaziri
    • 5
  • Françoise Munaut
    • 2
  • Anne-Marie Corbisier
    • 1
  • Joëlle Quetin-Leclercq
    • 4
  • Stéphane Declerck
    • 1
  1. 1.Earth and Life Institute, MycologyUniversité catholique de Louvain (UCL)LouvainBelgium
  2. 2.Mycothèque de l’Université catholique de Louvain (MUCL)Earth and Life Institute, Mycology, Université catholique de Louvain (UCL)LouvainBelgium
  3. 3.Laboratoire de microbiologie et de standardisation des médicamentsInstitut malgache de recherches appliquéesAntananarivoMadagascar
  4. 4.Laboratoire de PharmacognosieLouvain Drug Research Institute, Université catholique de LouvainBrusselsBelgium
  5. 5.Laboratoire de biotechnologie végétaleUniversité libre de BruxellesGosseliesBelgium

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