Anditalea andensis gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from extreme alkali–saline soil
- 296 Downloads
A novel alkalophilic salt-tolerant rod-shaped bacterium, designated ANESC-ST, was isolated from an extremely alkali–saline soil in the rural area of Anda city in northeast China. Taxonomic study using a polyphasic approach revealed that this non-motile, orange colony-forming microbe was Gram-negative and obligately aerobic. Optimal growth of strain ANESC-ST was achieved in the presence of NaCl with a concentration range of 0.5 to 4 % and pH between 7.5 and 9.2, and at temperatures ranging from 10 to 37 °C. Phylogenetic analysis of 16S rRNA sequences showed that of strain ANESC-ST is most homologous to Mongoliicoccus roseus MIM28T and Litoribacter ruber YIM CH208T with sequence similarity of 95.1 and 93.2 %, respectively. The genomic DNA G+C content of strain ANESC-ST was determined to be 39.1 mol%. The main isoprenoid quinone in ANESC-ST was found to be menaquinone-7. The main fatty acids were found to be iso-C15:0 (27.5 %), iso-C17:03-OH (14.0 %), anteiso-C15:0 (9.8 %), summed feature 9 (iso-C17:1ω9c and/or 10-methyl C16:0 10.6 %) and summed feature 3 (C16:1w7c/C16:1w6c, 9.78 %). Based on the phenotypic, chemotaxonomic and phylogenetic data, strain ANESC-ST is considered to represent a new genus and species classified into the order Cytophagales, for which the name Anditalea andensis gen. nov., sp. nov. is proposed. The type strain is ANESC-ST (=CICC 10485T = NCCB 100412T).
KeywordsExtreme alkaline-saline soil Alkaliphilic Anditalea andensis gen. nov. Polyphasic taxonomy
This work was supported by the National High Technology Research and Development Program (863 Project, 2002AA241111) and State Forestry Administration 948 Program of PR China (No. 2008429) to Shenkui Liu. We thank for the analytical services by the China Center of Industries Culture Collection (CICC) (Beijing, China).
- Anil Kumar P, Srinivas TN, Pavan Kumar P, Madhu S, Shivaji S (2010b) Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes. Int J Syst Evol Microbiol 60(Pt 10):2320–2325. doi: 10.1099/ijs.0.020230-0 PubMedCrossRefGoogle Scholar
- Dong XZ, Cai MY (2001) Identification manual of common bacteria. Science Press, Beijing, pp 349–399Google Scholar
- Griffiths RI, Whiteley AS, O’Donnell AG, Bailey MJ (2000) Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition. Appl Environ Microbiol 66(12):5488–5491. doi: 10.1128/aem.66.12.5488-5491.2000 PubMedCrossRefGoogle Scholar
- Ludwig W, Euze’ J, Whitman WB (2008) Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. Bergey’s Taxonomic Outlines, vol 4. http://ww.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf
- Nedashkovskaya OI, Ludwig W (2010) Family II. Cyclobacteriaceae fam. nov. In: Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ, Ward NL, Ludwig W, Whitman WB (eds) Bergey’s manual of systematic bacteriology, 2nd edn, vol 4. Springer, New York, pp 423Google Scholar
- Nedashkovskaya OI, Kim SB, Kwon KK, Shin DS, Luo X, Kim SJ, Mikhailov VV (2007) Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57(Pt 9):1988–1994. doi: 10.1099/ijs.0.65073-0 PubMedCrossRefGoogle Scholar
- Sasser M (1990). Identification of bacteria by gas chromatography of cellular fatty acids (technical note no. 101). MIDI Inc, Newark. http://www.midi-inc.com/media/pdfs/TechNote_101.pdf
- Shigematsu T, Tang Y, Kawaguchi H, Ninomiya K, Kijima J, Kobayashi T, Morimura S, Kida K (2003) Effect of dilution rate on structure of a mesophilic acetate-degrading methanogenic community during continuous cultivation. J Biosci Bioeng 96(6):547–558. doi: 10.1016/S1389-1723(04)70148-6 PubMedCrossRefGoogle Scholar
- Ying JY, Wang BJ, Yang SS, Liu SJ (2006) Cyclobacterium lianum sp. nov., a marine bacterium isolated from sediment of an oilfield in the South China Sea, and emended description of the genus Cyclobacterium. Int J Syst Evol Microbiol 56(Pt 12):2927–2930. doi: 10.1099/ijs.0.64510-0 PubMedCrossRefGoogle Scholar