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Antonie van Leeuwenhoek

, Volume 102, Issue 4, pp 703–710 | Cite as

Anditalea andensis gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from extreme alkali–saline soil

  • Wei Shi
  • Tetsuo Takano
  • Shenkui Liu
Original Paper

Abstract

A novel alkalophilic salt-tolerant rod-shaped bacterium, designated ANESC-ST, was isolated from an extremely alkali–saline soil in the rural area of Anda city in northeast China. Taxonomic study using a polyphasic approach revealed that this non-motile, orange colony-forming microbe was Gram-negative and obligately aerobic. Optimal growth of strain ANESC-ST was achieved in the presence of NaCl with a concentration range of 0.5 to 4 % and pH between 7.5 and 9.2, and at temperatures ranging from 10 to 37 °C. Phylogenetic analysis of 16S rRNA sequences showed that of strain ANESC-ST is most homologous to Mongoliicoccus roseus MIM28T and Litoribacter ruber YIM CH208T with sequence similarity of 95.1 and 93.2 %, respectively. The genomic DNA G+C content of strain ANESC-ST was determined to be 39.1 mol%. The main isoprenoid quinone in ANESC-ST was found to be menaquinone-7. The main fatty acids were found to be iso-C15:0 (27.5 %), iso-C17:03-OH (14.0 %), anteiso-C15:0 (9.8 %), summed feature 9 (iso-C17:1ω9c and/or 10-methyl C16:0 10.6 %) and summed feature 3 (C16:1w7c/C16:1w6c, 9.78 %). Based on the phenotypic, chemotaxonomic and phylogenetic data, strain ANESC-ST is considered to represent a new genus and species classified into the order Cytophagales, for which the name Anditalea andensis gen. nov., sp. nov. is proposed. The type strain is ANESC-ST (=CICC 10485T = NCCB 100412T).

Keywords

Extreme alkaline-saline soil Alkaliphilic Anditalea andensis gen. nov. Polyphasic taxonomy 

Notes

Acknowledgments

This work was supported by the National High Technology Research and Development Program (863 Project, 2002AA241111) and State Forestry Administration 948 Program of PR China (No. 2008429) to Shenkui Liu. We thank for the analytical services by the China Center of Industries Culture Collection (CICC) (Beijing, China).

Supplementary material

10482_2012_9770_MOESM1_ESM.doc (238 kb)
Supplementary material 1 (DOC 238 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Key Laboratory of Saline–alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of EducationAlkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry UniversityHarbinPeople’s Republic of China
  2. 2.State Key Laboratory of Forest Genetics and Tree BreedingNortheast Forestry UniversityHarbinPeople’s Republic of China
  3. 3.Asian Natural Environmental Science Center (ANESC)University of TokyoTokyoJapan

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