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Antonie van Leeuwenhoek

, Volume 102, Issue 3, pp 425–433 | Cite as

Extracellular sugar phosphates are assimilated by Streptomyces in a PhoP-dependent manner

  • Elodie Tenconi
  • Samuel Jourdan
  • Patrick Motte
  • Marie-Joëlle Virolle
  • Sébastien RigaliEmail author
Original Paper

Abstract

Filamentous microorganisms of the bacterial genus Streptomyces have a complex life cycle that includes physiological and morphological differentiations. It is now fairly well accepted that lysis of Streptomyces vegetative mycelium induced by programmed cell death (PCD) provides the required nutritive sources for the bacterium to erect spore-forming aerial hyphae. However, little is known regarding cellular compounds released during PCD and the contribution of these molecules to the feeding of surviving cells in order to allow them to reach the late stages of the developmental program. In this work we assessed the effect of extracellular sugar phosphates (that are likely to be released in the environment upon cell lysis) on the differentiation processes. We demonstrated that the supply of phosphorylated sugars, under inorganic phosphate limitation, delays the occurrence of the second round of PCD, blocks streptomycetes life cycle at the vegetative state and inhibits antibiotic production. The mechanism by which sugar phosphates affect development was shown to involve genes of the Pho regulon that are under the positive control of the two component system PhoR/PhoP. Indeed, the inactivation of the response regulator phoP of Streptomyces lividans prevented the ‘sugar phosphate effect’ whereas the S. lividans ppk (polyphosphate kinase) deletion mutant, known to overexpress the Pho regulon, presented an enhanced response to phosphorylated sugars.

Keywords

Sporulation Antibiotic production Programmed cell death Cannibalism Phosphate limitation 

Notes

Acknowledgments

We are thankful to Juan Francisco Martin, and Angel Manteca for interesting discussions. SR is a FRS-FNRS research associate. This work is supported by a FRIA fellowship from the FRS-FNRS to ET, by the ‘Fonds Spéciaux du Conseil de la Recherche’ from the University of Liège to PM and SR (grant 506 R.CFRA.1237), and by FRS-FNRS grants (Grant Nos. 2.4638.05, 2.4540.06, 2.4531.09, 2.4583.08 and 2.4581.10) to PM.

Supplementary material

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Supplementary material 1 (JPEG 129 kb)
10482_2012_9763_MOESM2_ESM.jpg (150 kb)
Supplementary material 2 (JPEG 149 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Elodie Tenconi
    • 1
  • Samuel Jourdan
    • 1
  • Patrick Motte
    • 1
    • 2
  • Marie-Joëlle Virolle
    • 3
  • Sébastien Rigali
    • 1
    Email author
  1. 1.Centre for Protein Engineering (CIP), Institut de Chimie B6aUniversity of LiègeLiègeBelgium
  2. 2.Laboratory of Functional Genomics and Plant Molecular Imaging and Centre for Assistance in Technology of Microscopy (CATM), Institute of Botany B22University of LiègeLiègeBelgium
  3. 3.Institut de Génétique et MicrobiologieUniversité Paris Sud 11Orsay CedexFrance

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