Description of Fimbriimonas ginsengisoli gen. nov., sp. nov. within the Fimbriimonadia class nov., of the phylum Armatimonadetes
- 821 Downloads
Strain Gsoil 348T was isolated from a ginseng field soil sample by selecting micro-colonies from one-fifth strength modified R2A agar medium after a long incubation period. 16S rRNA gene sequence analysis indicated that the strain is related to members of the phylum Armatimonadetes (formerly called candidate phylum OP10). Strain Gsoil 348T is mesophilic, strictly aerobic, non-motile and rod-shaped. It only grows in low nutrient media. The major respiratory quinones are menaquinones MK-11 and MK-10, and the main fatty acids are iso-C15:0, iso-C17:0, C16:0 and C16:1 ω11c. The G+C content is 61.4 mol%. The 16S rRNA gene sequences in public databases belonging to the phylum Armatimonadetes were clustered here into 6 groups. Five of these groups constituted a coherent cluster distinct from the sequences of other phyla in phylogenetic trees that were constructed using multiple-outgroup sequences from 49 different phyla. On the basis of polyphasic taxonomic analyses, it is proposed that strain Gsoil 348T (= KACC 14959T = JCM 17079T) should be placed in Fimbriimonas ginsengisoli gen. nov., sp. nov., as the cultured representative of the Fimbriimonadia class. nov., corresponding with Group 4 of the phylum Armatimonadetes.
KeywordsArmatimonadetes OP10 Phylogeny Novel class Fimbriimonadia
This work was supported by the Global Frontier Intelligent Biosystem Design and Synthesis Center Program, Ministry of Science and Technology and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea. We thank Jean Euzéby for his comments on specific etymology and nomenclature.
- Atlas RM (1993) Handbook of microbiological media. CRC Press, Boca RatonGoogle Scholar
- Lee KCY, Dunfield PF, Morgan XC, Crowe MA, Houghton KM, Vyssotski M, Ryan JLJ, Lagutin K, McDonald IR, Stott MB (2011) Chthonomonas calidirosea gen. nov., sp nov., an aerobic, pigmented, thermophilic micro-organism of a novel bacterial class, Chthonomonadetes classis nov., of the newly described phylum Armatimonadetes originally designated candidate division OP10. Int J Syst Evol Microbiol 61:2482–2490PubMedCrossRefGoogle Scholar
- Moore DD, Dowhan D (1995) Preparation and analysis of DNA. In: Ausubel FW, Brent R, Kingston RE et al (eds) Current protocols in molecular biology. Wiley, New York, pp 2–11Google Scholar
- Mori K, Yamaguchi K, Sakiyama Y, Urabe T, Suzuki K (2009) Caldisericum exile gen. nov., sp nov., an anaerobic, thermophilic, filamentous bacterium of a novel bacterial phylum, Caldiserica phyl. nov., originally called the candidate phylum OP5, and description of Caldisericaceae fam. nov., Caldisericales ord. nov and Caldisericia classis nov. Int J Syst Evol Microbiol 59:2894–2898PubMedCrossRefGoogle Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc, NewarkGoogle Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541PubMedCrossRefGoogle Scholar
- Widdel F, Bak F (1992) Gram-negative mesophilic sulfate reducing bacteria. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes. Springer, New YorkGoogle Scholar
- Zhang H, Sekiguchi Y, Hanada S, Hugenholtz P, Kim H, Kamagata Y, Nakamura K (2003) Gemmatimonas aurantiaca gen. nov., sp. nov., a gram-negative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum Gemmatimonadetes phyl. nov. Int J Syst Evol Microbiol 53:1155–1163PubMedCrossRefGoogle Scholar