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Antonie van Leeuwenhoek

, Volume 102, Issue 2, pp 297–305 | Cite as

Paenibacillus frigoriresistens sp. nov., a novel psychrotroph isolated from a peat bog in Heilongjiang, Northern China

  • Hong Ming
  • Guo-Xing Nie
  • Hong-Chen Jiang
  • Tian-Tian Yu
  • En-Min Zhou
  • Hui-Gen Feng
  • Shu-Kun Tang
  • Wen-Jun Li
Original Paper

Abstract

A novel cold-resistant bacterium, designated YIM 016T, was isolated from a peat bog sample collected from Mohe County, Heilongjiang Province, Northern China and its taxonomic position was investigated using a polyphasic approach. The strain was Gram-positive, aerobic, endospore-forming, motile and rod-shaped. Phylogenetic analyses based on the 16S rRNA gene sequence clearly revealed that strain YIM 016T is a member of the genus Paenibacillus. The strain is closely related to Paenibacillus alginolyticus DSM 5050T, Paenibacillus chondroitinus DSM 5051T and Paenibacillus pocheonensis Gsoil 1138T with similarities of 99.0 %, 97.0 % and 96.3 %, respectively. Meanwhile, the low DNA–DNA relatedness levels between strain YIM 016T and its closely related phylogenetic neighbours demonstrated that this isolate represents a new genomic species in the genus Paenibacillus. Phenotypic and chemotaxonomic tests showed that growth of strain YIM 016T occurred at 4–37 °C, pH 6.0–8.0 and with a NaCl tolerance up to 0.5 % (w/v). The peptidoglycan contained meso-diaminopimelic acid, alanine and glutamic acid. The whole-cell hydrolysates mainly contained glucose, galactose and ribose. The predominant menaquinone was MK-7 and the major fatty acids were anteiso-C15:0 and iso-C16:0. The DNA G+C content of strain YIM 016T was 51.7 mol %. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM 016T could be clearly distinguished from other species of the genus Paenibacillus. It is therefore concluded that strain YIM 016T represents a novel species in the genus Paenibacillus, for which the name Paenibacillus frigoriresistens sp. nov. is proposed. The type strain is YIM 016T (= CCTCC AB 2011150T = JCM 18141T).

Keywords

Paenibacillus frigoriresistens sp. nov. 16S rRNA gene Polyphasic taxonomy 

Notes

Acknowledgments

The authors are grateful to Prof. Dr. Hans-Peter Klenk (DSMZ) for his kind providing reference type strains and Prof. Jean Euzéby for the Latin construction of the species name. This research was supported by Science and Technology Innovation Talents Program in Universities of Henan Province of China (HASTIT, No. 2010HASTIT020), Key Technologies R & D Program of Henan Province of China (112102310335,112102210106), and Doctoral Fund of Ministry of Education of China (20100022120009). W-J. Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

Supplementary material

10482_2012_9738_MOESM1_ESM.docx (457 kb)
Supplementary material 1 (DOCX 457 kb)
10482_2012_9738_MOESM2_ESM.ppt (797 kb)
Supplementary material 2 (PPT 797 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Hong Ming
    • 1
    • 3
  • Guo-Xing Nie
    • 2
  • Hong-Chen Jiang
    • 4
  • Tian-Tian Yu
    • 3
  • En-Min Zhou
    • 3
  • Hui-Gen Feng
    • 1
  • Shu-Kun Tang
    • 3
  • Wen-Jun Li
    • 3
    • 5
  1. 1.Department of Life Sciences and TechnologyXinxiang Medical UniversityXinxiangPeople’s Republic of China
  2. 2.College of Life SciencesHenan Normal UniversityXinxiangPeople’s Republic of China
  3. 3.Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of MicrobiologyYunnan UniversityKunmingPeople’s Republic of China
  4. 4.State Key Laboratory of Biogeology and Environmental GeologyChina University of GeosciencesWuhanPeople’s Republic of China
  5. 5.Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of ScienceXinjiang Institute of Ecology and Geography, Chinese Academy of SciencesÜrümqiPeople’s Republic of China

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