Antonie van Leeuwenhoek

, Volume 102, Issue 2, pp 269–276 | Cite as

Streptosporangium anatoliense sp. nov., isolated from soil in Turkey

  • Anil Sazak
  • Nevzat Sahin
  • Mustafa Camas
  • Kiymet Guven
  • Demet Cetin
  • Michael Goodfellow
Original Paper

Abstract

A novel actinobacterium, strain N9999T, was isolated from soil and its taxonomic position determined using a polyphasic approach. The organism formed abundant aerial hyphae that differentiated into spherical spore vesicles. The cell wall contained meso-diaminopimelic acid; the whole-cell sugars were galactose, glucose, mannose, madurose and ribose; the predominant menaquinones MK-9 (H2) and MK-9 (H4); the major phospholipids phosphatidylethanolamine, diphosphatidylglycerol, a phosphaglycolipid and phosphatidylinositol mannosides; while the cellular fatty acids were rich in iso-C14:0, C15:0, cis-9-C17:1, iso-C16:0 and 10-methyl C17:0 components. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence indicated that strain N9999T was closely related to a group that consisted of Streptosporangiumpseudovulgare DSM 43181T and Streptosporangium nondiastaticum DSM 43848T. However, DNA–DNA relatedness and phenotypic data demonstrated that strain N9999T was clearly distinguished from all closely related Streptosporangium species. The combined genotypic and phenotypic data demonstrate conclusively that the isolate should be classified as a new species of Streptosporangium.

Keywords

Streptosporangiaceae Streptosporangium Streptosporangium anatoliense Polyphasic taxonomy 

Notes

Acknowledgments

This research was supported by Ondokuz Mayis University (OMU), project no. PYO. FEN. 1901.09.003. The authors’ thank Professor Jean Paul Marie Euzéby for his help with the nomenclature.

Supplementary material

10482_2012_9735_MOESM1_ESM.pdf (31 kb)
Supplementary material (PDF 31 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Anil Sazak
    • 1
  • Nevzat Sahin
    • 1
  • Mustafa Camas
    • 1
  • Kiymet Guven
    • 2
  • Demet Cetin
    • 3
  • Michael Goodfellow
    • 4
  1. 1.Department of Biology, Faculty of Art and ScienceOndokuz Mayis UniversityKurupelit-SamsunTurkey
  2. 2.Department of Biology, Faculty of ScienceAnadolu UniversityEskisehirTurkey
  3. 3.Science Teaching Programme, Gazi Faculty of EducationGazi UniversityAnkaraTurkey
  4. 4.School of BiologyUniversity of NewcastleNewcastle upon TyneUK

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