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Antonie van Leeuwenhoek

, Volume 102, Issue 1, pp 83–89 | Cite as

Cohnella plantaginis sp. nov., a novel nitrogen-fixing species isolated from plantain rhizosphere soil

  • Li-Ying Wang
  • San-Feng ChenEmail author
  • Lei Wang
  • Yu-Guang Zhou
  • Hong-Can Liu
Original Paper

Abstract

A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83T, is Gram-positive, aerobic, motile and rod-shaped (0.4–0.6 μm × 1.9–2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83T was a member of the genus Cohnella. High similarity of 16S rRNA gene sequence was found between YN-83T and Cohnella ginsengisoli DSM18997T (97.99%), whereas the similarity was below 96.0% between YN-83T and the other Cohnella species. DNA–DNA relatedness between strain YN-83T and C. ginsengisoli DSM18997T was 27.4 ± 6.2%. The DNA G+C content of strain YN-83T was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C15:0 (44.3%), iso-C15:0 (11.3%), iso-C16:0 (18.6%) and C16:0 (7.7%). The polar lipids of strain YN-83T consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA–DNA hybridization, strain YN-83T represents a novel species of the genus Cohnella, for which the name Cohnella plantaginis sp. nov. (type strain YN-83T = DSM 25424T = CGMCC 1.12047T) is proposed.

Keywords

Cohnella plantaginis sp. nov Nitrogen-fixing Cohnella 

Notes

Acknowledgments

We thank Professor Ji Li in Department of Ecology and Ecological Engineering of China Agricultural University for generous help; we are grateful to Professor Qixiao Li in Department of Molecular Biosciences and Bioengineering of University of Hawaii for modifying the article; we also thank Dr. Soon-Wo Kwon in Korea for generous gift the reference type strain; This work was supported by the National Natural Science Foundation of China (Grant No. 31070082) and the national “973” Project (Grant No. 2010CB126504).

Supplementary material

10482_2012_9715_MOESM1_ESM.docx (141 kb)
Supplementary material 1 (DOCX 141 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Li-Ying Wang
    • 1
  • San-Feng Chen
    • 1
    Email author
  • Lei Wang
    • 1
  • Yu-Guang Zhou
    • 2
  • Hong-Can Liu
    • 2
  1. 1.State Key Laboratory for Agrobiotechnology, Department of Microbiology and Immunology, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.China General Microbiological Culture Collection Center, Institute of MicrobiologyChinese Academy of SciencesBeijingChina

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