Verrucosispora wenchangensis sp. nov., isolated from mangrove soil
- 278 Downloads
An actinomycete strain 234402T was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402T indicated that the highest similarity was to Verrucosispora sediminis MS426T (99.25%). The cell wall contained meso-diaminopimelic acid. The major menaquinones were MK-9(H4) and MK-9(H6), with MK-9(H8) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA–DNA relatedness demonstrated strain 234402T could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402T represents a novel species of the genus Verrucosispora, for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402T (=CCTCC AA 2011018T=DSM 45674T).
KeywordsVerrucosispora wenchangensis sp. nov Polyphasic taxonomy 16S rRNA
This investigation was co-supported by National Natural Science Foundation of China (31170467), and National Nonprofit Institute Research Grant of CATAS-ITBB (ITBBZD0753). The authors thank Mr Wenquan Li for his help on sample collection.
- Felsenstein J (2008) PHYLIP (phylogeny inference package) Version 3.68, Department of Genome Sciences, University of Washington, SeattleGoogle Scholar
- Gordon RE (1967) The taxonomy of soil bacteria. In: Gray TRG and Parkinson D (eds) Ecology of soil bacteria. Liverpool University Press, Liverpool, pp 293–321Google Scholar
- Kämpfer P, Kroppenstedt RM, Dott W (1991) A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137:1831–1891Google Scholar
- Kelly KL (1964) Inter-society color council—National Bureau of standards color name charts illustrated with centroid colors. US Government Printing Office, Washington, DCGoogle Scholar
- Lechevalier MP, Lechevalier HA (1980) The chemotaxonomy of actinomycetes. In: Dietz A, Arlington TJ (eds) Actinomycete taxonomy (special publication no. 6). Society for Industrial Microbiology, Fairfax, pp. 227–291Google Scholar