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Antonie van Leeuwenhoek

, Volume 102, Issue 1, pp 1–7 | Cite as

Verrucosispora wenchangensis sp. nov., isolated from mangrove soil

  • Qing-yi Xie
  • Hai-peng Lin
  • Lei Li
  • Roselyn Brown
  • Michael Goodfellow
  • Zixin Deng
  • Kui Hong
Original Paper

Abstract

An actinomycete strain 234402T was isolated from a mangrove soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 234402T indicated that the highest similarity was to Verrucosispora sediminis MS426T (99.25%). The cell wall contained meso-diaminopimelic acid. The major menaquinones were MK-9(H4) and MK-9(H6), with MK-9(H8) as minor components. The characteristic whole-cell sugars were xylose, mannose and glucose. The phospholipid profile was found to comprise phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unknown phospholipid. The DNA G+C content was 69.2 mol%. The results of physiological and biochemical tests and low DNA–DNA relatedness demonstrated strain 234402T could be readily distinguished from the closely related Verrucosispora species. On the basis of these phenotypic and genotypic data, strain 234402T represents a novel species of the genus Verrucosispora, for which the name Verrucosispora wenchangensis sp. nov. is proposed. The type strain is 234402T (=CCTCC AA 2011018T=DSM 45674T).

Keywords

Verrucosispora wenchangensis sp. nov Polyphasic taxonomy 16S rRNA 

Notes

Acknowledgment

This investigation was co-supported by National Natural Science Foundation of China (31170467), and National Nonprofit Institute Research Grant of CATAS-ITBB (ITBBZD0753). The authors thank Mr Wenquan Li for his help on sample collection.

Supplementary material

10482_2012_9707_MOESM1_ESM.docx (21 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Qing-yi Xie
    • 2
  • Hai-peng Lin
    • 2
  • Lei Li
    • 1
    • 2
  • Roselyn Brown
    • 3
  • Michael Goodfellow
    • 3
  • Zixin Deng
    • 1
  • Kui Hong
    • 1
    • 2
  1. 1.Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of EducationWuhan University School of Pharmaceutical SciencesWuhanPeople’s Republic of China
  2. 2.Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouPeople’s Republic of China
  3. 3.School of BiologyUniversity of NewcastleNewcastle upon TyneUK

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