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Antonie van Leeuwenhoek

, Volume 101, Issue 4, pp 845–857 | Cite as

Spatial and seasonal prokaryotic community dynamics in ponds of increasing salinity of Sfax solar saltern in Tunisia

  • Ines Boujelben
  • María Gomariz
  • Manuel Martínez-GarcíaEmail author
  • Fernando Santos
  • Arantxa Peña
  • Cristina López
  • Josefa Antón
  • Sami Maalej
Original Paper

Abstract

The spatial and seasonal dynamics of the halophilic prokaryotic community was investigated in five ponds from Sfax solar saltern (Tunisia), covering a salinity gradient ranging from 20 to 36%. Fluorescence in situ hybridization indicated that, above 24% salinity, the prokaryotic community shifted from bacterial to archaeal dominance with a remarkable increase in the proportion of detected cells. Denaturing gradient gel electrophoresis (DGGE) profiles were rather similar in all the samples analyzed, except in the lowest salinity pond (around 20% salt) where several specific archaeal and bacterial phylotypes were detected. In spite of previous studies on these salterns, DGGE analysis unveiled the presence of microorganisms not previously described in these ponds, such as Archaea related to Natronomonas or bacteria related to Alkalimnicola, as well as many new sequences of Bacteroidetes. Some phylotypes, such as those related to Haloquadratum or to some Bacteroidetes, displayed a strong dependence of salinity and/or magnesium concentrations, which in the case of Haloquadratum could be related to the presence of ecotypes. Seasonal variability in the prokaryotic community composition was focused on two ponds with the lowest (20%) and the highest salinity (36%). In contrast to the crystallized pond, where comparable profiles between autumn 2007 and summer 2008 were obtained, the non-crystallized pond showed pronounced seasonal changes and a sharp succession of “species” during the year. Canonical correspondence analysis of biological and physicochemical parameters indicated that temperature was a strong factor structuring the prokaryotic community in the non-crystallizer pond, that had salinities ranging from 20 to 23.8% during the year.

Keywords

Solar saltern Hypersaline environment Environmental factors Prokaryotic dynamics DGGE FISH Haloquadratum Bacteroidetes 

Notes

Acknowledgments

This work was funded by projects A/6679/06 from the Spanish Agency for International Cooperation (AECI) and CGL2009-12651-C02-01 from the Spanish Ministry of Science and Innovation (MICINN). We also wish to acknowledge the Tunisian COTUSAL salt company in Sfax for allowing members of our research team to access to the saltern.

Supplementary material

10482_2012_9701_MOESM1_ESM.doc (526 kb)
Supplementary material 1 (DOC 524 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ines Boujelben
    • 1
    • 2
  • María Gomariz
    • 1
    • 3
  • Manuel Martínez-García
    • 1
    Email author
  • Fernando Santos
    • 1
  • Arantxa Peña
    • 1
  • Cristina López
    • 1
  • Josefa Antón
    • 1
  • Sami Maalej
    • 2
  1. 1.Departamento de Fisiología, Genética y MicrobiologíaUniversidad de AlicanteAlicanteSpain
  2. 2.Département des Sciences de la Vie, Faculté des Sciences de SfaxUniversité de SfaxSfaxTunisia
  3. 3.Departamento de Microbiología y Producción VegetalUniversidad Miguel Hernández de ElcheElcheSpain

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